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To study the epidemiology of carbapenem resistant E. coli isolates from piglet faecal samples a cross section study was conducted in 10 different locations and total of 673 faecal swabs were collected. On preliminary screening with 1 mg/L imipenem added MacConkey ag 112 E. coli isolates were isolated. The phenotypic screening of 112 isolates with carbapenem antibiotics, aztreonam and moxalactam showed resistance of 18.80 % for meropenem, 9.80% for imipenem, 16.1 % ertapenem, 14.30 % aztreonam and 9.80% for moxalactam. Out of 112 E. coli isolates, 79 (70.54 %) had meropenem MIC between 1 mig…mehr

Produktbeschreibung
To study the epidemiology of carbapenem resistant E. coli isolates from piglet faecal samples a cross section study was conducted in 10 different locations and total of 673 faecal swabs were collected. On preliminary screening with 1 mg/L imipenem added MacConkey ag 112 E. coli isolates were isolated. The phenotypic screening of 112 isolates with carbapenem antibiotics, aztreonam and moxalactam showed resistance of 18.80 % for meropenem, 9.80% for imipenem, 16.1 % ertapenem, 14.30 % aztreonam and 9.80% for moxalactam. Out of 112 E. coli isolates, 79 (70.54 %) had meropenem MIC between 1 mig and 2 mig /mL (sensitive) and 10 (8.92%) had MIC between 2 and 4 mig/mL (intermediate) and 23 isolates (20.54 %) had meropenem MIC more than 4 mig/mL (resistant). Twenty three isolates were carbapenem resistant, out of which 8 isolates showed keyhole reaction with EDTA and on genotypic screening all were NDM positives. The efflux pump screening of 15 carbapenemase genes negative isolates, 8 isolates showed efflux pump mediated resistance. The remaining 7 isolates showed neither carbapenemase gene nor efflux pump mediated resistance.
Autorenporträt
Dr Pruthvishree B.S. currently pursuing research at the Division of Epidemiology, Indian Veterinary Research Institute, India. She does research in Epidemiology and antimicrobial resistance.