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The present study was designed to investigate the diagnostic value and determine whether any of serum procalcitonin level, neutrophils CD64 and lymphocytes CD69 markers as predictor for neonatal sepsis prior to the blood culture, as well as identify the genetic susceptibility of CD14/C-159T gene polymorphism to sepsis in neonates by identification the genotypic and allelic frequency of CD14/C-159T polymorphism and the association of its susceptibility to sepsis in neonate.