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This classic and bestselling landmark publication, originally published in 1965, examines the dynamic mechanisms, fundamental principles, and physical properties of various chromatographic procedures. It offers methods to characterize, identify, and predict chromatographic phenomena - providing strategies to select the most appropriate separation tools and techniques for specific applications in chemistry, physics, biology, and forensic and environmental science. It contains many worked equations, real-world examples, and schematic figures of key concepts, covers control migration rate differences and zone spreading, analyzes flow, diffusion, and kinetic events, and summarizes mathematical quantities and parameters.