Development of novel droplet-based microfluidic strategies for the molecular diagnosis of cancer
Deniz Pekin
Broschiertes Buch

Development of novel droplet-based microfluidic strategies for the molecular diagnosis of cancer

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The aim of this work is to establish novel strategies for the highly sensitive screening of cancer biomarkers in biological samples.To achieve this goal, we developed droplet-based microfluidic dPCR technique. Using a limiting dilution, individual DNA molecules are encapsulated within monodisperse droplets of a water-in-oil emulsion created with a microfluidic device. Fluorescent TaqMan probes targeting the screened cancer biomarkers allow the detection of mutations. We focused on the mutations in the human KRAS gene for the development of our test. This method is also transposable in a multip...