Matthew Linman, Quan Cheng

Developing Novel Biointerfaces and Biomaterials with SPR/SPR Imaging

Expanding the Limits of SPR Spectroscopy and SPR Imaging

• Broschiertes Buch

Produktbeschreibung

Much research has focused on the development of analytical methods to monitor a multitude of biological interactions including protein-carbohydrate and protein-lipid among others. Surface plasmon resonance (SPR) remains one of the most exciting techniques that allows for real-time data collection and label free detection amenable to many biological systems. This thesis focuses on the development of novel sensing interfaces/materials for biomolecular interaction analysis (BIA) with SPR and SPR imaging (SPRi). The first portion of this work focuses on the development of unique surface chemistry designs to examine biological interactions including the development of a high-density array to detect carbohydrate-lectin interactions with SPRi. The second part of this thesis focuses on the development of new substrates for both SPR and SPR imaging experiments. The fabrication of a nanometric thick layer of glass-on-gold to detect protein-lipid interactions with SPR is presented. Finally,the facile fabrication of gold-coated etched glass substrates for SPR imaging that reduces background resonance 5-fold in situ compared to the standard gold island array platform is presented as well.

Produktdetails

• Verlag: LAP Lambert Academic Publishing
• Aufl.
• Seitenzahl: 300
• Erscheinungstermin: 30. Januar 2012
• Englisch
• Abmessung: 220mm x 150mm x 18mm
• Gewicht: 414g
• ISBN-13: 9783847321880
• ISBN-10: 3847321889
• Artikelnr.: 34907290

Autorenporträt

Matthew Linman, Ph.D. is currently an Engineering Scientist at Meso Scale Diagnostics in Maryland, USA. He received his Ph.D. in analytical chemistry from the University of California-Riverside in 2011 and his bachelors from the College of Wooster in 2005. He is an author on nearly 20 scientific publications including 2 book chapters.