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Oyster Mushroom spawns were brought from Tamil Nadu Agriculture University, Coimbatore. Mushroom bags were made from polythene in the size of 50x50 cm. Different agro waste substrates were used to grow oyster mushroom. Different agro waste substrates were used to grow oyster mushroom. Such as, Paddy straw, Coconut Coir, Sugarcane biogases and sawdust respectively. Water is irrigated in the method of spray irrigation once at a day. Mushroom were harvested after 15 days of cultivation. pin head appearance (10 days), stipe length (2.8 cm), cap diameter (7.8 cm) found. Besides Paddy straw (1500g) and Coconut coir (1000 g) were found good substrates in terms of yield of mushroom. Saw dust was found poor substrate in comparison to other conventional substrate because here yield was 800g was obtained. The dry matter of the three mushroom species examined ranged between 7-9%.It is important to consider moisture content, since all of the nutrients are contained in the dry matter of food. Dry matter levels as high as 20% have been reported for some species, however 10% or less is common.