S Bradbury and P J Evennett

Contrast Techniques in Light Microscopy

• Broschiertes Buch

Produktbeschreibung

Contrast in an image is essential to distinguish features from one another and from the background. Contrast Techniques in Light Microscopy is a practical handbook describing the ways in which light interacts with the specimen in the microscope and the manner in which this is used to generate contrast in the image of the specimen. The book shows ways in which such interactions are used in practice and includes examples and case studies of contrast enhancement. The book describes techniques for both transmitted and reflected light, as well as the effects of the refractive index of the mounting medium surrounding the specimen. Techniques covered include bright field and dark ground, polarized light, phase contrast, modulation contrast, interference and fluorescence, and also contrast manipulation outside the microscope. Contrast Techniques in Light Microscopy is an essential practical guide for all microscopists.

Produktdetails

• Verlag: CRC Press
• Seitenzahl: 136
• Erscheinungstermin: 15. Juni 1996
• Englisch
• Abmessung: 234mm x 156mm x 7mm
• Gewicht: 200g
• ISBN-13: 9781859960851
• ISBN-10: 1859960855
• Artikelnr.: 21761223

Autorenporträt

Savile Bradbury (Author) , Peter Evennett (Author)

Inhaltsangabe

Introduction
Fundamental considerations
Bright-field and dark-ground techniques
Refraction differences between specimen and mountant
the 'Becke Line'
Polarized-light techniques
Phase contrast and modulation contrast
Interference contrast
Fluoresc