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Ganodermalucidum, a white rot fungus belonging to basidiomycetes group, which is widely distributed worldwide, was studied for the production of the lignin-modifying enzymelaccase. In this experiment laccase production was investigated using submerged fermentation. The effects of variations in the media nutrient composition or culture conditions were provided to increase the production of laccase at laboratory level. The conditions for maximum production of Laccase by G. lucidum have been optimized in shake flask studies. The parameters like temperature, pH, Inoculum size and Incubation period were studied. Maximum Laccase activity was obtained at 25°C (11.852U/ml) and 6 pH (12.077U/ml). In case of Inoculum size and incubation period 5 discs and 7 days were optimum parameters for maximum laccase activity (12.410U/ml) and (12.635U/ml) respectively.