Cloning, Expression and Purification of kir6.1 in Bacterial system

Cloning, Expression and Purification of kir6.1 in Bacterial system

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Adenosine-triphosphate-sensitive K+channels [K+(ATP)] are the protein channels responsive to changes in ATP/adenosine diphosphate (ADP) ratio and provide a means to couple movement of potassium ions, to relate membrane potential with cellular energy status. Various subfamilies of K+(ATP) channel proteins, designated as Kir1.0 to 7.0, share significant amino-acid homology (40%) among them. The Kir6.0 subfamily consists of Kir6.1 and Kir6.2 proteins with around 70% amino acid homology. Kir6.1 is widely expressed in tissues, including pancreatic islets, heart, skeletal muscle and brain. K+(ATP) c...