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A number of chromatographic techniques have been developed for the purpose of separation of mixed substances. Chromatography is the most functional and widely used method in chemical testing and experiments. It plays an important role in the development of chemistry, biology and medicine and more such related areas. Its elementary simplicity and simple operation make it versatile to apply along with other detection methods such as electrolysis, photometric methods etc. This book overviews latest developments in the techniques of chromatography which would prove to be valuable to the readers involved in separation science. It covers three major areas i.e. theories, its applications and sample preparations. This book targets not just students but also practitioners and professionals engaged in the field of chemistry, pharmacology, biotechnology and related genres.