Jiaqian Wu

CHARACTERIZE MAMMALIAN TRANSCRIPTOME COMPLEXITY

FROM GENOME-WIDE RT-PCR CDNA CLONING, TO NOVEL MAMMALIAN GENE DISCOVERY, AND TRANSCRIPTOME COMPLEXITY

• Broschiertes Buch

Produktbeschreibung

In my Ph.D. thesis, I described a large scale RT-PCR and cloning pipeline that I developed to obtain several thousand full-length mammalian (mainly human and mouse) cDNA clones for the NHGRI/NCI Mammalian Gene Collection effort. At that time, the layers of the mammalian transcriptome complexity were not yet fully conceived. In this project, I was deeply involved in strategic and conceptual issues in characterizing transcriptome complexity from different levels. Using the RT-PCR pipeline combined with gene prediction algorithms that utilize comparative genomic information, we successfully identified and experimentally verified novel mammalian genes such as full-length rat homologs of human disease genes. Furthermore, I studied transcriptome complexity including SNPs and alternative splicing in leukemia samples using quantitative PCR, and we developed a trace analysis technology to identify multiple alternative spliced transcripts in one single sequencing reaction. My results suggested that alternatively spliced transcripts show cancer type specific up- or down- regulation, and the ratio of alternative spliced isoforms may underlie the cancer mechanism.

Produktdetails

• Verlag: LAP Lambert Academic Publishing
• Seitenzahl: 204
• Erscheinungstermin: 29. April 2011
• Englisch
• Abmessung: 220mm x 150mm x 13mm
• Gewicht: 322g
• ISBN-13: 9783844320602
• ISBN-10: 3844320601
• Artikelnr.: 33355835

Autorenporträt

El Dr. Jiaqian Wu es autor de muchas publicaciones revisadas por colegas en el campo de la genética, la genómica y la investigación con células madre. Ganó varios premios y tiene una patente. Jiaqian ha dado charlas en conferencias internacionales e institutos de investigación. Actualmente, Jiaqian es profesor adjunto en la Facultad de Medicina de la Universidad de Texas en Houston.