Characterization of the Angiotensin receptor AT2 by various studies
Chirag Mandavia
Broschiertes Buch

Characterization of the Angiotensin receptor AT2 by various studies

Signal transduction, ligand-binding properties and protein interaction studies of the Angiotensin AT2 receptor

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In order to study AT2-mediated signal transduction mechanisms, two main approaches were used: (1) the ligand-binding properties of mutant AT2 receptors were characterized in comparison to wild-type after generation of a point mutation using site-directed mutagenesis, and (2) interacting protein partners were analyzed using yeast library screening, co-immunoprecipitation and Western Blot studies. We found that the ability of Angiotensin II (Ang II) receptor type AT2 Phe308Ala residue mutant in the 7th TMD to bind peptidic ligands was greatly reduced, using Xenopus oocytes expression system. Thi...