Cell and Molecular Biology of the Cytoskeleton focuses on the three major fibrous proteins in the cytoplasm that are collectively known as the cytoskeletal sys tem. These polymorphic cytoskeletal proteins are microtubules (25-nm diam eter), microfilaments (6-nm diameter), and intermediate filaments (l O-nm diameter). Microtubules consist of tubulin and several well-characterized mi crotubule-associated proteins (MAPs) such as MAPl, MAP2, and tau. Micro filaments consist of actin and associate with actin-binding proteins, including alpha-actinin, filamin, myosin, tropomyosin, vinculin, and…mehr
Cell and Molecular Biology of the Cytoskeleton focuses on the three major fibrous proteins in the cytoplasm that are collectively known as the cytoskeletal sys tem. These polymorphic cytoskeletal proteins are microtubules (25-nm diam eter), microfilaments (6-nm diameter), and intermediate filaments (l O-nm diameter). Microtubules consist of tubulin and several well-characterized mi crotubule-associated proteins (MAPs) such as MAPl, MAP2, and tau. Micro filaments consist of actin and associate with actin-binding proteins, including alpha-actinin, filamin, myosin, tropomyosin, vinculin, and others. Intermedi ate filaments consist of at least five different tissue-specific classes, including desmin or skeletin (muscle), prekeratin (epithelial), vimentin (mesenchymal), neurofilament (nerve), and glial acidic fibrillary protein (astrocytes). In this volume distinguished researchers in the field cover the interaction of these fibrous proteins, not only with each other and other cytoplasmic components, but also with such biological processes as cell shape changes, growth, motility, secretion, and division. These comprehensive reviews ex plore the cytoskeleton's molecular, biochemical, and structural properties with an emphasis on their manifestation in the living cell.
1 Probing the Cytoskeleton by Microinjection.- 1. Introduction.- 2. The Microtubule System.- 2.1. Analyzing the Equilibrium between Tubulin and Microtubules.- 2.2. Studying Microtubular Dynamics.- 2.3. Analyzing Microtubule-Bound Organelle Transport.- 2.4. Introducing Antitubulins.- 3. The Microfilament System.- 3.1. Studying Actin Dynamics.- 3.2. Studying the Dynamics of Actin-Associated Proteins.- 3.3. Reorganizing Microfilament Patterns by Introducing Structural Proteins.- 3.4. Introducing Phalloidin.- 3.5. Using Antibodies against Microfilament Proteins.- 4. The Intermediate Filament System.- 4.1. Introducing Antibodies.- 4.2. Introducing mRNA.- 5. The Coated Vesicle System.- 6. Conclusion.- References.- 2 Structural Aspects of Intermediate Filaments.- 1. Introduction.- 2. Domain Organization of IF Proteins.- 3. Neurofilaments.- 4. Organization of the Sequences along the Rod Domain.- 5. Comparison of the Six Nonepithelial Proteins along the Rod Domain.- 6. Structural Characterization of the Rod.- 7. From the Rodlike Protofilament to the 10-nm Filament.- 8. Structure and Function of the Terminal Domains in IF Proteins.- 8.1. The Involvement of Headpieces in Filament Formation.- 8.2. Structural Characterization of the Tailpieces.- 8.3. Are the Carboxyterminal Tailpieces Appendages Communicating between Stiff Filaments and the Cytoplasm?.- 9. Addendum.- References.- 3 The Structure and Evolution of Intermediate Filament Genes.- 1. Introduction.- 1.1. Correlation of Keratin Gene Expression with Differentiation State.- 1.2. Correlation of Keratin Subunit Structure with Differential Expression.- 2. Structure of Intermediate Filament Genes.- 3. A Model for the Evolution of Intermediate Filament Genes.- 3.1. Evolution of the Rod Domains of Intermediate Filament Genes.- 3.2. Evolution of the End Domains.- 4. Conclusion.- References.- 4 Differential Expression of the Genes Encoding the Keratins of Cultured Human Epidermal Cells.- 1.Introduction.- 2.The Structure of the 8-nm Keratin Filaments of Epidermis.- 3. The Genes Encoding the Human Epidermal Keratins.- 4. Structure of the Two Types of Keratin Genes and Their Relation to Other Intermediate-Filament Genes.- 5. Regulation of the Type I and Type II Keratin Genes.- References.- 5 Organization and Expression of the Vimentin and Desmin Genes.- 1. The Discovery of Intermediate Filaments.- 2. The Diversity of IF Subunits.- 3. The Genes Encoding IF.- 4. Expression of the Cloned Desmin Gene.- 4.1. In Lens Cells.- 4.2. Expression of the Hamster Desmin Gene in Human Epithelial (HeLa) Cells.- 5. Chromosome Localization of the Human Vimentin and Desmin Gene.- References.- 6 Actin: A Regulator of Cell Growth and Differentiation.- 1. Introduction.- 2. Cell Morphology and Growth and Differentiation.- 2.1. Cell Configuration.- 2.2. Cytoskeletal Organization.- 3. Regulation of Actin Gene Expression.- 4. Is Actin a Protooncogene?.- 5. Mechanisms by Which Actin May Regulate Cell Growth and Differentiation.- References.- 7 The Actin Genes in Caenorhabditis elegans.- 1. Introduction.- 2. Genomic Organization.- 3. Sequence Analysis.- 4. Transcription.- 5. Translational Products.- 6. Identification of Actin Mutants.- 7. Actin Gene Organization in Revertants of Actin Mutants.- 8. Transcription in Revertants.- 9. Future Prospects.- References.- 8 Implications of Microtubule Polarity for Microtubule Function.- 1. Introduction.- 2. Background and Methods for Determining MT Polarity.- 3. MT Polarity and Spindle Function.- 4. MT Polarity and Intracellular Motility.- References.- 9 Molecular Mechanisms Controlling Tubulin Synthesis.- 1. Introduction.- 2. Apparent Autoregulatory Control of Tubulin Synthesis in Animal Cells.- 2.1. Tubulin Synthesis in Animal Cells Is Sensitive to the Apparent Pool of Unpolymerized Subunits.- 2.2. Possible Molecular Levels for Regulating Tubulin Synthetic Rates.- 2.3. Transient Induction of Tubulin Synthesis by Interferon.- 3. Programmed Synthesis of Tubulin during Flagellar
1 Probing the Cytoskeleton by Microinjection.- 1. Introduction.- 2. The Microtubule System.- 2.1. Analyzing the Equilibrium between Tubulin and Microtubules.- 2.2. Studying Microtubular Dynamics.- 2.3. Analyzing Microtubule-Bound Organelle Transport.- 2.4. Introducing Antitubulins.- 3. The Microfilament System.- 3.1. Studying Actin Dynamics.- 3.2. Studying the Dynamics of Actin-Associated Proteins.- 3.3. Reorganizing Microfilament Patterns by Introducing Structural Proteins.- 3.4. Introducing Phalloidin.- 3.5. Using Antibodies against Microfilament Proteins.- 4. The Intermediate Filament System.- 4.1. Introducing Antibodies.- 4.2. Introducing mRNA.- 5. The Coated Vesicle System.- 6. Conclusion.- References.- 2 Structural Aspects of Intermediate Filaments.- 1. Introduction.- 2. Domain Organization of IF Proteins.- 3. Neurofilaments.- 4. Organization of the Sequences along the Rod Domain.- 5. Comparison of the Six Nonepithelial Proteins along the Rod Domain.- 6. Structural Characterization of the Rod.- 7. From the Rodlike Protofilament to the 10-nm Filament.- 8. Structure and Function of the Terminal Domains in IF Proteins.- 8.1. The Involvement of Headpieces in Filament Formation.- 8.2. Structural Characterization of the Tailpieces.- 8.3. Are the Carboxyterminal Tailpieces Appendages Communicating between Stiff Filaments and the Cytoplasm?.- 9. Addendum.- References.- 3 The Structure and Evolution of Intermediate Filament Genes.- 1. Introduction.- 1.1. Correlation of Keratin Gene Expression with Differentiation State.- 1.2. Correlation of Keratin Subunit Structure with Differential Expression.- 2. Structure of Intermediate Filament Genes.- 3. A Model for the Evolution of Intermediate Filament Genes.- 3.1. Evolution of the Rod Domains of Intermediate Filament Genes.- 3.2. Evolution of the End Domains.- 4. Conclusion.- References.- 4 Differential Expression of the Genes Encoding the Keratins of Cultured Human Epidermal Cells.- 1.Introduction.- 2.The Structure of the 8-nm Keratin Filaments of Epidermis.- 3. The Genes Encoding the Human Epidermal Keratins.- 4. Structure of the Two Types of Keratin Genes and Their Relation to Other Intermediate-Filament Genes.- 5. Regulation of the Type I and Type II Keratin Genes.- References.- 5 Organization and Expression of the Vimentin and Desmin Genes.- 1. The Discovery of Intermediate Filaments.- 2. The Diversity of IF Subunits.- 3. The Genes Encoding IF.- 4. Expression of the Cloned Desmin Gene.- 4.1. In Lens Cells.- 4.2. Expression of the Hamster Desmin Gene in Human Epithelial (HeLa) Cells.- 5. Chromosome Localization of the Human Vimentin and Desmin Gene.- References.- 6 Actin: A Regulator of Cell Growth and Differentiation.- 1. Introduction.- 2. Cell Morphology and Growth and Differentiation.- 2.1. Cell Configuration.- 2.2. Cytoskeletal Organization.- 3. Regulation of Actin Gene Expression.- 4. Is Actin a Protooncogene?.- 5. Mechanisms by Which Actin May Regulate Cell Growth and Differentiation.- References.- 7 The Actin Genes in Caenorhabditis elegans.- 1. Introduction.- 2. Genomic Organization.- 3. Sequence Analysis.- 4. Transcription.- 5. Translational Products.- 6. Identification of Actin Mutants.- 7. Actin Gene Organization in Revertants of Actin Mutants.- 8. Transcription in Revertants.- 9. Future Prospects.- References.- 8 Implications of Microtubule Polarity for Microtubule Function.- 1. Introduction.- 2. Background and Methods for Determining MT Polarity.- 3. MT Polarity and Spindle Function.- 4. MT Polarity and Intracellular Motility.- References.- 9 Molecular Mechanisms Controlling Tubulin Synthesis.- 1. Introduction.- 2. Apparent Autoregulatory Control of Tubulin Synthesis in Animal Cells.- 2.1. Tubulin Synthesis in Animal Cells Is Sensitive to the Apparent Pool of Unpolymerized Subunits.- 2.2. Possible Molecular Levels for Regulating Tubulin Synthetic Rates.- 2.3. Transient Induction of Tubulin Synthesis by Interferon.- 3. Programmed Synthesis of Tubulin during Flagellar
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