Celiac Disease, Volume 179 in the Methods in Cell Biology series is composed of extensive protocols about novel and widely used techniques for celiac disease research. Topics covered in this volume include Pepsin trypsin digested gliadin treatment of intestinal cells in vitro, Ex vivo Gliadin Stimulation of Intestinal Cells, Measuring intestinal permeability in celiac disease ex vivo using Ussing chambers, Intestinal permeability assessment using lactulose and mannitol, In vivo sensitization to gliadin by oral administration, Separation of epithelial and immune cells from intestinal biopsy…mehr
Celiac Disease, Volume 179 in the Methods in Cell Biology series is composed of extensive protocols about novel and widely used techniques for celiac disease research. Topics covered in this volume include Pepsin trypsin digested gliadin treatment of intestinal cells in vitro, Ex vivo Gliadin Stimulation of Intestinal Cells, Measuring intestinal permeability in celiac disease ex vivo using Ussing chambers, Intestinal permeability assessment using lactulose and mannitol, In vivo sensitization to gliadin by oral administration, Separation of epithelial and immune cells from intestinal biopsy samples, Isolation and study of dendritic cells and macrophages from human intestinal samples, Gnobiotic mouse models to study gluten sensitivity, and more. Other sections in this new release include In vitro differentiation of macrophages from peripheral blood cells of celiac patients, Assessment of activated gut-homing CD8+ T cells in blood by flow cytometry during a three-day gluten challenge, Isolation, cryopreservation and thawing of peripheral blood mononuclear cells for downstream applications, and more.
1. Pepsin-trypsin digested gliadin treatment in intestinal cells Ane Olazagoitia-Garmendia 2. Ex vivo gliadin stimulation of intestinal cells Linda Zhang and Chuan He 3. Measuring intestinal permeability in celiac disease ex vivo, using Ussing chambers Olga Biskou and Amaia Jauregi-Miguel 4. Intestinal permeability assessment using lactulose and mannitol in celiac disease Silvia Martinez Velasco, Azucena Gonzalez Garcia, Inaki Xarles Irastorza Terradillos, and Jose Ramon Bilbao Catala 5. In vivo sensitization to gliadin by oral administration M. Mar Romero, Dolors Serra, Ainara Castellanos-Rubio, and Laura Herrero 6. Separation of epithelial and immune cells from biopsy samples Irati Romero-Garmendia 7. Study and isolation of human intestinal dendritic cell and macrophage subsets Elisa Arribas-Rodriguez, Luis Fernandez-Salazar, Beatriz de Andres, Eduardo Arranz, Jose A. Garrote, and David Bernardo 8. Elucidating the role of microbes in celiac disease through gnotobiotic modeling Alberto Caminero, Elena F. Verdu, and Heather J. Galipeau 9. In vitro differentiation of macrophages from peripheral blood cells of celiac patients Federica Farina, Mariavittoria Laezza, Alessio Fasano, and Giovanna Del Pozzo 10. Assessment of activated gut-homing CD8+ T cells in blood by flow cytometry during a 3-day gluten challenge Concepcion Nuñez, Sara Gomez-Aguililla, Maria Corzo, F. Fernandez-Bañares, A. Bodas, S. Farrais, N. Lopez-Palacios, and Mercedes Rubio 11. Isolation and cryopreservation of peripheral blood mononuclear cells Sara Gomez-Aguililla, Concepcion Nuñez, Mercedes Rubio, and Maria Corzo 12. Flow cytometric analysis of duodenal intraepithelial lymphocytes (celiac lymphogram): A diagnostic test for celiac disease Concepcion Nuñez, A. Carrasco, Maria Corzo, R. Pariente, M. Esteve, and G. Roy 13. The HLA-DQ8 transgenic mouse: A model to study the immune and cytotoxic responses to wheat gliadin Francesco Maurano, Vera Rotondi Aufiero, Lucia Treppiccione, Stefano Rossi, Diomira Luongo, Giuseppe Mazzarella, and Mauro Rossi 14. Human intestinal organoid models for celiac disease research Valeriia Dotsenko, Amir-Babak Sioofy-Khojine, Heikki Hyoty, and Keijo Viiri 15. Preparation of pepsin trypsin digested gliadin for stimulation experiments Luis Manuel Mendoza-Gomez, Maialen Sebastian-delaCruz, Ane Olazagoitia-Garmendia, Itziar Gonzalez-Moro, Henar Rojas-Marquez, Jon Mentxaka, Izortze Santin, and Ainara Castellanos-Rubio 16. A fast, cheap, and easy protocol for celiac disease HLA haplotype typing using buccal swabs Maialen Sebastian-delaCruz and Ainara Castellanos-Rubio
1. Pepsin-trypsin digested gliadin treatment in intestinal cells Ane Olazagoitia-Garmendia 2. Ex vivo gliadin stimulation of intestinal cells Linda Zhang and Chuan He 3. Measuring intestinal permeability in celiac disease ex vivo, using Ussing chambers Olga Biskou and Amaia Jauregi-Miguel 4. Intestinal permeability assessment using lactulose and mannitol in celiac disease Silvia Martinez Velasco, Azucena Gonzalez Garcia, Inaki Xarles Irastorza Terradillos, and Jose Ramon Bilbao Catala 5. In vivo sensitization to gliadin by oral administration M. Mar Romero, Dolors Serra, Ainara Castellanos-Rubio, and Laura Herrero 6. Separation of epithelial and immune cells from biopsy samples Irati Romero-Garmendia 7. Study and isolation of human intestinal dendritic cell and macrophage subsets Elisa Arribas-Rodriguez, Luis Fernandez-Salazar, Beatriz de Andres, Eduardo Arranz, Jose A. Garrote, and David Bernardo 8. Elucidating the role of microbes in celiac disease through gnotobiotic modeling Alberto Caminero, Elena F. Verdu, and Heather J. Galipeau 9. In vitro differentiation of macrophages from peripheral blood cells of celiac patients Federica Farina, Mariavittoria Laezza, Alessio Fasano, and Giovanna Del Pozzo 10. Assessment of activated gut-homing CD8+ T cells in blood by flow cytometry during a 3-day gluten challenge Concepcion Nuñez, Sara Gomez-Aguililla, Maria Corzo, F. Fernandez-Bañares, A. Bodas, S. Farrais, N. Lopez-Palacios, and Mercedes Rubio 11. Isolation and cryopreservation of peripheral blood mononuclear cells Sara Gomez-Aguililla, Concepcion Nuñez, Mercedes Rubio, and Maria Corzo 12. Flow cytometric analysis of duodenal intraepithelial lymphocytes (celiac lymphogram): A diagnostic test for celiac disease Concepcion Nuñez, A. Carrasco, Maria Corzo, R. Pariente, M. Esteve, and G. Roy 13. The HLA-DQ8 transgenic mouse: A model to study the immune and cytotoxic responses to wheat gliadin Francesco Maurano, Vera Rotondi Aufiero, Lucia Treppiccione, Stefano Rossi, Diomira Luongo, Giuseppe Mazzarella, and Mauro Rossi 14. Human intestinal organoid models for celiac disease research Valeriia Dotsenko, Amir-Babak Sioofy-Khojine, Heikki Hyoty, and Keijo Viiri 15. Preparation of pepsin trypsin digested gliadin for stimulation experiments Luis Manuel Mendoza-Gomez, Maialen Sebastian-delaCruz, Ane Olazagoitia-Garmendia, Itziar Gonzalez-Moro, Henar Rojas-Marquez, Jon Mentxaka, Izortze Santin, and Ainara Castellanos-Rubio 16. A fast, cheap, and easy protocol for celiac disease HLA haplotype typing using buccal swabs Maialen Sebastian-delaCruz and Ainara Castellanos-Rubio
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