Capillary electrophoresis combines the analytical separation technique of classical electrophoresis with the instrumental potential of modern chromatographic detection and automation. It eminently supplements chromatography for the separation of polar and water-soluble substances. The extent of its applications is extremely broad, spanning the range of separation from small cations to the highest molecular eight ionic biopolymers. The breadth of its application potential has now made capillary electrophoresis the fastedgrowing area of instrumental analysis. This book provides a practical…mehr
Capillary electrophoresis combines the analytical separation technique of classical electrophoresis with the instrumental potential of modern chromatographic detection and automation. It eminently supplements chromatography for the separation of polar and water-soluble substances. The extent of its applications is extremely broad, spanning the range of separation from small cations to the highest molecular eight ionic biopolymers. The breadth of its application potential has now made capillary electrophoresis the fastedgrowing area of instrumental analysis. This book provides a practical introduction to capillary electrophoresis separation techniques. Partticular value is placed on the developing and optimizing a separation. Insofar as possible, intructive exemples were selected for the explanation of these processes, without recourse to mathematical details. It is hoped that this short introduction will facilitate the beginner's entry and use of this efficient methodology.
1 Introduction.- 2 Principles of Capillary Electrophoresis.- 3 Theoretical Foundations and Their Influence on the Analytical Results.- 3.1 Electrophoretic Migration.- 3.2 Conductivity.- 3.3 Electroosmotic Flow.- 3.4 Band Broadening.- 4 Instrumentation.- 4.1 Power Supply.- 4.2 Capillaries.- 4.3 Sample Introduction.- 4.4 Thermostating.- 4.5 Detection.- 4.6 Special Problems of Quantitative Analysis in CE.- 5 Capillary Zone Electrophoresis (CZE).- 5.1 Principles of Optimization in CZE.- 5.2 Indirect Detection Methods in CE.- 5.3 Capillary Zone Electrophoresis of Proteins.- 6 Micellar Electrokinetic Chromatography (MEKC).- 6.1 Fundamentals of MEKC.- 6.2 Optimization of Resolution.- 6.3 Selection of the Detergent.- 6.4 Separations by MEKC.- 7 Separation of Enantiomers by CE.- 7.1 Enantiomeric Separations with Cyclodextrins as Chiral Selectors.- 7.2 Other Separation Systems.- 8 Capillary Gel Electrophoresis (CGE).- 8.1 Acrylamide-based Gels.- 8.2 Polysaccharide-based Gels and other Polymers.-8.3 Migration Models of Biopolymers in Polymer Solutions.- 9 Isoelectric Focusing in Capillaries (CIEF).- 10 Other Separation Techniques in CE.- 10.1 Isotachophoresis (ITP).- 10.2 Electrochromatography (EC).- 11 A Troubleshooting Guide to CE.- 11.1 Determination of the Problem Source.- 11.2 Scenarios of Problems: "What to do if...".- 12 Literature Index.- 12.1 Literature Cited.- 12.2 Additional Literature Sources.- 13 Acknowledgement.
1 Introduction.- 2 Principles of Capillary Electrophoresis.- 3 Theoretical Foundations and Their Influence on the Analytical Results.- 3.1 Electrophoretic Migration.- 3.2 Conductivity.- 3.3 Electroosmotic Flow.- 3.4 Band Broadening.- 4 Instrumentation.- 4.1 Power Supply.- 4.2 Capillaries.- 4.3 Sample Introduction.- 4.4 Thermostating.- 4.5 Detection.- 4.6 Special Problems of Quantitative Analysis in CE.- 5 Capillary Zone Electrophoresis (CZE).- 5.1 Principles of Optimization in CZE.- 5.2 Indirect Detection Methods in CE.- 5.3 Capillary Zone Electrophoresis of Proteins.- 6 Micellar Electrokinetic Chromatography (MEKC).- 6.1 Fundamentals of MEKC.- 6.2 Optimization of Resolution.- 6.3 Selection of the Detergent.- 6.4 Separations by MEKC.- 7 Separation of Enantiomers by CE.- 7.1 Enantiomeric Separations with Cyclodextrins as Chiral Selectors.- 7.2 Other Separation Systems.- 8 Capillary Gel Electrophoresis (CGE).- 8.1 Acrylamide-based Gels.- 8.2 Polysaccharide-based Gels and other Polymers.-8.3 Migration Models of Biopolymers in Polymer Solutions.- 9 Isoelectric Focusing in Capillaries (CIEF).- 10 Other Separation Techniques in CE.- 10.1 Isotachophoresis (ITP).- 10.2 Electrochromatography (EC).- 11 A Troubleshooting Guide to CE.- 11.1 Determination of the Problem Source.- 11.2 Scenarios of Problems: "What to do if...".- 12 Literature Index.- 12.1 Literature Cited.- 12.2 Additional Literature Sources.- 13 Acknowledgement.
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