Calcium-Binding Protein Protocols

Volume 2: Methods and Techniques
Herausgegeben:Vogel, Hans J.

• Broschiertes Buch

Produktbeschreibung

Calcium plays an important role in a wide variety of biological processes. This divalent metal ion can bind to a large number of proteins; by doing so it modifies their biological activity or their stability. Because of its distinct che- cal properties calcium is uniquely suited to act as an on-off switch or as a light dimmer of biological activities. The two books entitled Calcium-Binding Protein Protocols (Volumes I and II) focus on modern experimental analyses and methodologies for the study of calcium-binding proteins. Both extracel- lar and intracellular calcium-binding proteins are discussed in detail. H- ever, proteins involved in calcium handling (e. g. , calcium pumps and calcium channels), fall outside of the scope of these two volumes. Also, calcium-bi- ing proteins involved in bone deposition will not be discussed, as this specific topic has been addressed previously. The focus of these two books is on studies of the calcium-binding proteins and their behavior in vitro and in vivo. The primary emphasis is on protein chemistry and biophysical methods. Many of the methods described will also be applicable to proteins that do not bind calcium. Calcium-Binding Protein Protocols is divided into three main sections. The section entitled Introduction and Reviews provides information on the role of calcium in intracellular secondary messenger activation mechanisms. Mo- over, unique aspects of calcium chemistry and the utilization of calcium in dairy proteins, as well as calcium-binding proteins involved in blood clotting, are addressed.

Produktdetails

• Methods in Molecular Biology 173
• Verlag: Humana / Humana Press / Springer, Berlin
• Artikelnr. des Verlages: 978-1-61737-136-3
• Seitenzahl: 415
• Erscheinungstermin: 10. November 2010
• Englisch
• Abmessung: 229mm x 152mm x 25mm
• Gewicht: 628g
• ISBN-13: 9781617371363
• ISBN-10: 161737136X
• Artikelnr.: 32201619

Inhaltsangabe

Methods and Techniques to Study.- Quantitative Analysis of Ca2+-Binding by Flow Dialysis.- Calcium Binding to Proteins Studied via Competition with Chromophoric Chelators.- Deconvolution of Calcium-Binding Curves.- Absorption and Circular Dichroism Spectroscopy.- Fourier Transform Infrared Spectroscopy of Calcium-Binding Proteins.- Steady-State Fluorescence Spectroscopy.- Fluorescence Methods for Measuring Calcium Affinity and Calcium Exchange with Proteins.- Surface Plasmon Resonance of Calcium-Binding Proteins.- Differential Scanning Calorimetry.- Isothermal Titration Calorimetry.- Multiangle Laser Light Scattering and Sedimentation Equilibrium.- Small-Angle Solution Scattering Reveals Information on Conformational Dynamics in Calcium-Binding Proteins and in their Interactions with Regulatory Targets.- Investigation of Calcium-Binding Proteins Using Electrospray Ionization Mass Spectrometry.- Synthetic Calcium-Binding Peptides.- Proteolytic Fragments of Calcium-Binding Proteins.- Electron Magnetic Resonance Studies of Calcium-Binding Proteins.- Cadmium-113 and Lead-207 NMR Spectroscopic Studies of Calcium-Binding Proteins.- Calcium-43 NMR of Calcium-Binding Proteins.- Exploring Familial Relationships Using Multiple Sequence Alignment.- Structure Determination by NMR.- Protein Structure Calculation from NMR Data.- Shape and Dynamics of a Calcium-Binding Protein Investigated by Nitrogen-15 NMR Relaxation.- The Use of Dipolar Couplings for the Structure Refinement of a Pair of Calcium-Binding EGF Domains.- Vector Geometry Mapping.- Use of Calmodulin Antagonists and S-100 Protein Interacting Drugs for Affinity Chromatography.- Enzymatic Assays to Compare Calmodulin Isoforms, Mutants, and Chimeras.- Gene Expression inTransfected Cells.- Monitoring the Intracellular Free Ca2+-Calmodulin Concentration with Genetically-Encoded Fluorescent Indicator Proteins.- Studying the Spatial Distribution of Ca2+-Binding Proteins.