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Mastitis is an inflammatory condition of the mammary gland, characterized by changes in the physical characteristics of the udder or milk (Nazifi et al., 2011). Mastitis can be classified into three major types: clinical mastitis (CM), subclinical mastitis (SCM) and chronic mastitis (ChM) (Anonymous, 2003). In CM, there is swelling, heat, pain, and indurations observed in the mammary gland and also changes in milk color, clots are present in the milk and there are large numbers of leukocytes in the milk (Sharma and Maiti, 2010). In SCM, there are no gross inflammatory changes in the udder tissue and no changes physical appearance on milk. Only increased somatic cell count (SCC) in the milk, the presence of pathogenic organisms in the milk, and an inflammatory response that can only be detected by screening tests such as California Mastitis Test (CMT), White Side Test (WST), Surf Field Mastitis Test (SFMT) (Sachin and Suresh 2006; Madut et al., 2009). In ChM, udder becomes hard due to fibrosis, the quarters may become thickened, firm, nodular and atrophic.