Biological Low-Voltage Scanning Electron Microscopy

Herausgegeben:Pawley, James; Schatten, Heide

• Broschiertes Buch

Produktbeschreibung

Major improvements in instrumentation and specimen preparation have brought SEM to the fore as a biological imaging technique. Although this imaging technique has undergone tremendous developments, it is still poorly represented in the literature, limited to journal articles and chapters in books. This comprehensive volume is dedicated to the theory and practical applications of FESEM in biological samples. It provides a comprehensive explanation of instrumentation, applications, and protocols, and is intended to teach the reader how to operate such microscopes to obtain the best quality images.

Produktdetails

• Verlag: Springer / Springer New York / Springer, Berlin
• Artikelnr. des Verlages: 978-1-4899-9584-1
• 2008
• Seitenzahl: 344
• Erscheinungstermin: 13. September 2014
• Englisch
• Abmessung: 235mm x 155mm x 19mm
• Gewicht: 528g
• ISBN-13: 9781489995841
• ISBN-10: 1489995846
• Artikelnr.: 41625861

Inhaltsangabe

Table of Contents1. Low Voltage Scanning Electron Microscopy In Biology--Historical Preface :The Early Development of the Scanning Electron Microscope to 1965...1Dennis McMullan2. LVSEM For Biology...27James B. Pawley, PhD3. The Aberration-Corrected SEM...101David Joy Science and Engineering Research Facility, University of Tennessee4. Noise and its effects on the Low Voltage SEM...121David C Joy5. High-Resolution Low Voltage Field-Emission Scanning Electron Microscopy (HRLVFESEM) Applications for Cell Biology and Specimen Preparation Protocols...146Heide Schatten, PhD6. Molecular labeling for Correlative Microscopy: LM, LVSEM, TEM, EF-TEM and HVEM...173Ralph Albrecht and Daryl Meyer7. Low kV and video-rate, beam-tilt stereo for viewing live-time experiments in the SEM...209Alan Boyde8. CryoSEM of Chemically Fixed Animal Cells...228Stanley L. Erlandsen 9. High resolution and low voltage SEM of plant cells...249Guy Cox, Peter Vesk, Teresa Dibbayawan, Tobias I. Baskin and Maret Vesk10. High Resolution Cryo-Scanning Electron Microscopy of Biological Samples...268Paul Walther11. Developments in Instrumentation for Microanalysis in Low Voltage Scanning Electron Microscopy...305Dale E. Newbury

Rezensionen

From the reviews:

"Topics ranging from the early development of the scanning electron microscope (SEM) to some of the latest low-voltage and cryo techniques are reviewed in detail. ... this is a very useful volume for those wishing to learn, or refresh their memory, in the context of getting the best out of an SEM, and in particular the field emission SEM (FESEM)." (Iolo Ap Gwynn, infocus Magazine, Issue 12, December, 2008)
"Biological Low-voltage Scanning Electron Microscopy is an excellent and timely collection, written by many of the recognized experts in the subject ... . Strongly recommended." (Ultramicroscopy, Vol. 109, June, 2009)