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Canola (Brassica napus) is an important oilseed with worldwide it is currently ranked in third position, after soybean and palm oils, and in fifth position over the world trade in agricultural crops, after rice, wheat, maize and cotton. Canola has been introduced to Egypt more recently as a promising new vegetable oil crop especially in the new reclaimed lands. One of foreign canola seed cultivars (Pactol) and other one of local cultivars (Cerro-4) were subjected to the oil extraction process and the extracted oil characteristics were studied. Moreover, anti-nutritional factors of isolated protein were determined, then removed to prepare the isolated protein for rats feeding experiment. The aim of the present study is an attempt to removal of phenolic compounds in canola meal by different methods and preparation protein isolate. In addition to that biological evaluation of replacement of casein with canola protein isolate on experimental rats.