Modeled in its aims and graphic presentation on the supremely successful manual Biotechnology for Beginners, this book is angled at students and higher-education instructors of medicine, biotechnology, biochemistry, chemistry and the ecological sciences; medical technologists; lab technicians; biology and chemistry teachers; and readers of Scientific American looking for a readily understandable introduction to biological analysis methods and their applications. Largely speaking, it deals with bioanalytic facts, examining the way they interconnect and the background knowledge required to…mehr
Modeled in its aims and graphic presentation on the supremely successful manual Biotechnology for Beginners, this book is angled at students and higher-education instructors of medicine, biotechnology, biochemistry, chemistry and the ecological sciences; medical technologists; lab technicians; biology and chemistry teachers; and readers of Scientific American looking for a readily understandable introduction to biological analysis methods and their applications. Largely speaking, it deals with bioanalytic facts, examining the way they interconnect and the background knowledge required to understand them, yet it also discusses the economic and ethical pros and cons involved. The text is easy to read and strongly geared to teaching the subject. Further, the wide range of pictorial features makes it especially interesting and entertaining. The layout is based on that of Biotechnology for Beginners. This manual is an excellent preparation for the Spektrum book on bioanalysis as well as the experimentation volumes from the same publisher.
Reinhard Renneberg has been a Professor of Analytic Biotechnology at the Hong Kong University of Science and Technology (www.ust.hk) since 1955. He is the author of the extremely successful and much-lauded volume Biotechnology for Beginners, of which over 5,300 copies have been sold in 18 months. He has also written three other books on biotechnology, including Katzenklon. He is a co-author of the Roempp Biotechnology Dictionary, has written four monographs and 250 publications and holds 20 patents. In addition, he is involved in two biotechnology companies in Germany and China.
Inhaltsangabe
PREFACE CHAPTER 1 The Nanoru - The incredible story of its isolation, purification, and characterization 1.1 The Site 1.2 Rearing and pure culture 1.3 Biomass production 1.4 Activity Test 1.5 Gel and ion exchange chromatography 1.6 Affinity chromatography 1.7 Isoelectric focusing 1.8 Gel electrophoresis 1.9 Mass and sequence analysis 1.10 How the Gen was "fished" 1.11 X-ray structure and NMR 1.12 The Sensation: The Nanoru - sudden clarity 1.13 How do we continue with the Nanoru? CHAPTER 2 Biomolecules in tests - Instrumental Bioanalytics 2.1 More and more machines for ever-smaller particles 2.2 Protein separation 2.3 Gel filtration chromatography separates proteins by size 2.4 Ion exchange chromatography separates proteins after their charge 2.5 Molecular Ying and Yang: affinity chromatography 2.6 High-performance liquid chromatography (HPLC) 2.7 What occurs with purification? Electrophoresis analyses protein mixtures qualitatively 2.8 Isoelectric focusing separates proteins according to their neutral points 2.9 Capillary electrophoresis combines high selectivity with brief periods of separation 2.10 Antibody probes identify proteins 2.11 The instrumental exploration of protein structure 2.12 Edman - Sequencing deciphered the primary structure of a protein 2.13 Mass spectrometry determined exact protein and peptide primary structure 2.14 X-ray structure deciphered protein conformation 2.15 The nuclear resonance spectroscopy (NMR) studied proteins in solution CHAPTER 3 Biocatalysis - enzymes and enzyme tests 3.1 Enzymes: highly specific and efficient molecular machines 3.2 Chicken or egg? Ribozymes are also biocatalysts 3.3 How enzymes recognize substrates 3.4 How enzymes are named and classified 3.5 Key-lock or hand glove? 3.6 Coenzymes are transformed like substrates 3.7 Enzyme kinetics: how enzyme reactions are in the time progress 3.8 Unit and Katal: the measurement of enzyme activity 3.9 Getting started: optical enzyme tests 3:10 Dry chemistry: from the litmus test paper for glucose-testing 3.11 Inhibition of enzyme reactions 3.12 Bird dead or : the exact measurement of enzyme inhibitors 3.13 Isoenzymes 3.14 Enzyme-activity tests CHAPTER 4 Bio-affinity I: antibodies and immune tests 75 4.1 Is War vaccination successful? The ring test 4.2 How antigens and antibodies react with Haptene 4.3 Blood is a very special juice: determination of blood groups 4.4 Soluble + soluble = insoluble: immune precipitation 4.5 Diffusion combined with electrophoresis: immune electrophoresis 4.6 Useful protein blurs: Western blotting 4.7 Nephelometry: to fish in the dark with success 4.8 Immuno assays: "The best [perfect] is the enemy of the good" 4.9 Thyroid tests with the Radio immuno assay 4.10 Immunology with the power of enzymes: ELISA 4.11 Indirect ELISA: detection of antibodies against HIV and the Dot test 4.12 Rapid immuno-tests: a baby yes or no? 4.13 The rapid detection of a death angel: HIV tests 4.14 Quick help with heart attacks 4.15 A worldwide trend: Point of care (POC) tests 4.16 Drugs and their abuse 4.17 Immunological drug tests 4.18 How to evaluate a laboratory test: sample HIV testing 4.19 How to test tests: ROC-curves CHAPTER 5 Bio-affinity II: biological receptors - nature as unsurpassed Bioanalysis lab worker 5.1 The fantastic dog nose: A million-times better than ours! 5.2 Our human meaning 5.3 Smell: olfactory detection 5.4 How does a receptor work? 5.5 Electronic nose: Combined polymers versus real receptors 5.6 Taste: gustatory detection 5.7 Visual detection 5.8 The evolution of the eye 5.9 Processes in the retina 5.10 Seeing colours 5.11 Listen: acoustic detection 5.12 Molecular mechanisms of hearing 5.13 Touch: tactile detection 5.14 Are there any other sensory systems? CHAPTER 6 DNA, RNA, and their amplification 6.1 DNA: the double helix 6.2 Rools for DNA analysis: DNA polymerase 6.3 DNA to RNA: RNA polymerase 6.4 In short: the DN
PREFACE CHAPTER 1 The Nanoru - The incredible story of its isolation, purification, and characterization 1.1 The Site 1.2 Rearing and pure culture 1.3 Biomass production 1.4 Activity Test 1.5 Gel and ion exchange chromatography 1.6 Affinity chromatography 1.7 Isoelectric focusing 1.8 Gel electrophoresis 1.9 Mass and sequence analysis 1.10 How the Gen was "fished" 1.11 X-ray structure and NMR 1.12 The Sensation: The Nanoru - sudden clarity 1.13 How do we continue with the Nanoru? CHAPTER 2 Biomolecules in tests - Instrumental Bioanalytics 2.1 More and more machines for ever-smaller particles 2.2 Protein separation 2.3 Gel filtration chromatography separates proteins by size 2.4 Ion exchange chromatography separates proteins after their charge 2.5 Molecular Ying and Yang: affinity chromatography 2.6 High-performance liquid chromatography (HPLC) 2.7 What occurs with purification? Electrophoresis analyses protein mixtures qualitatively 2.8 Isoelectric focusing separates proteins according to their neutral points 2.9 Capillary electrophoresis combines high selectivity with brief periods of separation 2.10 Antibody probes identify proteins 2.11 The instrumental exploration of protein structure 2.12 Edman - Sequencing deciphered the primary structure of a protein 2.13 Mass spectrometry determined exact protein and peptide primary structure 2.14 X-ray structure deciphered protein conformation 2.15 The nuclear resonance spectroscopy (NMR) studied proteins in solution CHAPTER 3 Biocatalysis - enzymes and enzyme tests 3.1 Enzymes: highly specific and efficient molecular machines 3.2 Chicken or egg? Ribozymes are also biocatalysts 3.3 How enzymes recognize substrates 3.4 How enzymes are named and classified 3.5 Key-lock or hand glove? 3.6 Coenzymes are transformed like substrates 3.7 Enzyme kinetics: how enzyme reactions are in the time progress 3.8 Unit and Katal: the measurement of enzyme activity 3.9 Getting started: optical enzyme tests 3:10 Dry chemistry: from the litmus test paper for glucose-testing 3.11 Inhibition of enzyme reactions 3.12 Bird dead or : the exact measurement of enzyme inhibitors 3.13 Isoenzymes 3.14 Enzyme-activity tests CHAPTER 4 Bio-affinity I: antibodies and immune tests 75 4.1 Is War vaccination successful? The ring test 4.2 How antigens and antibodies react with Haptene 4.3 Blood is a very special juice: determination of blood groups 4.4 Soluble + soluble = insoluble: immune precipitation 4.5 Diffusion combined with electrophoresis: immune electrophoresis 4.6 Useful protein blurs: Western blotting 4.7 Nephelometry: to fish in the dark with success 4.8 Immuno assays: "The best [perfect] is the enemy of the good" 4.9 Thyroid tests with the Radio immuno assay 4.10 Immunology with the power of enzymes: ELISA 4.11 Indirect ELISA: detection of antibodies against HIV and the Dot test 4.12 Rapid immuno-tests: a baby yes or no? 4.13 The rapid detection of a death angel: HIV tests 4.14 Quick help with heart attacks 4.15 A worldwide trend: Point of care (POC) tests 4.16 Drugs and their abuse 4.17 Immunological drug tests 4.18 How to evaluate a laboratory test: sample HIV testing 4.19 How to test tests: ROC-curves CHAPTER 5 Bio-affinity II: biological receptors - nature as unsurpassed Bioanalysis lab worker 5.1 The fantastic dog nose: A million-times better than ours! 5.2 Our human meaning 5.3 Smell: olfactory detection 5.4 How does a receptor work? 5.5 Electronic nose: Combined polymers versus real receptors 5.6 Taste: gustatory detection 5.7 Visual detection 5.8 The evolution of the eye 5.9 Processes in the retina 5.10 Seeing colours 5.11 Listen: acoustic detection 5.12 Molecular mechanisms of hearing 5.13 Touch: tactile detection 5.14 Are there any other sensory systems? CHAPTER 6 DNA, RNA, and their amplification 6.1 DNA: the double helix 6.2 Rools for DNA analysis: DNA polymerase 6.3 DNA to RNA: RNA polymerase 6.4 In short: the DN
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