Basic Biotechniques for Bioprocess and Bioentrepreneurship deals with the entire field of industrial biotechnology, starting from the basic laboratory techniques to scale-up, process development, demonstration, and finally its commercialization. The book compiles currently scattered materials on this topic and updates this information based on practical experience and requirements. The book will be an ideal source for new entrepreneurs who wish to start their own commercial units.
Basic Biotechniques for Bioprocess and Bioentrepreneurship deals with the entire field of industrial biotechnology, starting from the basic laboratory techniques to scale-up, process development, demonstration, and finally its commercialization. The book compiles currently scattered materials on this topic and updates this information based on practical experience and requirements. The book will be an ideal source for new entrepreneurs who wish to start their own commercial units.
Part I: Isolation screening and culture maintenance 1. Isolation of microorganisms 2. Screening strategies 3. Identification morphological biochemical and genetic characterization of microorganisms 4. Microbial activity and productivity enhancement strategies 5. Culture maintenance preservation and strain improvement Part II: Laboratory techniques and instrumentation 6. Biomolecules: Types homogenization bead beater sonication 7. Centrifugation: Basic principle types 8. Spectroscopy-Principle types and applications 9. Protein purification: Basic principles and techniques 10. Chromatography: Basic principle types and applications 11. Electrophoresis: Basic principle types and applications Part III: Genomic and proteomic analysis 12. DNA RNA isolation primer designing sequence submission and phylogenetic analysis 13. RDT and genetic engineering: Basic of RDT method PCR and application 14. Protein sequence analysis 15. Computational strategies and tools for protein tertiary structure prediction 16. Docking strategies 17. A beginner's guide to measuring binding affinity during biomolecular interactions Part IV: Industrially important enzymes 18. Enzymes and their significance in the industrial bioprocesses 19. Enzyme kinetics: Industrially important enzymes 20. Industrial enzymes: Basic information assay and applications Part V: Techniques in process development 21. Fundamentals of fermentation technology 22. Sterilization in bioprocesses 23. Scale-up: Lab to commercial scale 24. Bioprocess: Control management and biosafety issues 25. Demonstration and industrial scale-up 26. Downstream processing of biotechnology products 27. Waste management and environment Part VI: Feasibility marketing and biobusiness 28. Biobusiness opportunities 29. Feasibility analysis and business plan 30. Patenting IPR and regulatory issues 31. Commercialization and technology transfers of bioprocess 32. Bioproduct marketing strategies
Part I: Isolation screening and culture maintenance 1. Isolation of microorganisms 2. Screening strategies 3. Identification morphological biochemical and genetic characterization of microorganisms 4. Microbial activity and productivity enhancement strategies 5. Culture maintenance preservation and strain improvement Part II: Laboratory techniques and instrumentation 6. Biomolecules: Types homogenization bead beater sonication 7. Centrifugation: Basic principle types 8. Spectroscopy-Principle types and applications 9. Protein purification: Basic principles and techniques 10. Chromatography: Basic principle types and applications 11. Electrophoresis: Basic principle types and applications Part III: Genomic and proteomic analysis 12. DNA RNA isolation primer designing sequence submission and phylogenetic analysis 13. RDT and genetic engineering: Basic of RDT method PCR and application 14. Protein sequence analysis 15. Computational strategies and tools for protein tertiary structure prediction 16. Docking strategies 17. A beginner's guide to measuring binding affinity during biomolecular interactions Part IV: Industrially important enzymes 18. Enzymes and their significance in the industrial bioprocesses 19. Enzyme kinetics: Industrially important enzymes 20. Industrial enzymes: Basic information assay and applications Part V: Techniques in process development 21. Fundamentals of fermentation technology 22. Sterilization in bioprocesses 23. Scale-up: Lab to commercial scale 24. Bioprocess: Control management and biosafety issues 25. Demonstration and industrial scale-up 26. Downstream processing of biotechnology products 27. Waste management and environment Part VI: Feasibility marketing and biobusiness 28. Biobusiness opportunities 29. Feasibility analysis and business plan 30. Patenting IPR and regulatory issues 31. Commercialization and technology transfers of bioprocess 32. Bioproduct marketing strategies
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