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The etiology of bacterial organisms in otitis media vary from continent to continent i.e. locality to locality. This variation can be attributed to the emergence of increasing antimicrobial resistance, the difference in social-cultural practices, nutrition, and socioeconomic factors among others. This research aimed at finding out the most affected gender, the prevalence of bacterial otitis media, the most common bacterial isolates and their antibiogram pattern of patients presenting with otitis media at the Ear Nose and Throat clinic and the Immunosuppressed clinic in Mbarara National Referral Hospital.