Bacterial Cell Wall Homeostasis

Methods and Protocols
Herausgegeben:Hong, Hee-Jeon

• Broschiertes Buch

Produktbeschreibung

This volume bringstogether the most widely used and important protocols currently being employedin researching and understanding bacterial cell wall homeostasis. Chapters in BacterialCell Wall Homeostasis cover a variety of subjects, such as: modernmicroscopy techniques and other biophysical methods used to characterize thesubcellular structure of the bacterial cell wall; high-throughput approachesthat can be used to identify all the genes and proteins that participate in thecorrect functioning of an organism's cell wall; protocols for assayingindividual gene products for specific cell wall functions or identify chemicalswith inhibitory activity against the cell wall; and methods for analyzing the non-proteincomponents of the cell wall and the increasing use of computational approachesfor predicting and modeling cell wall related functions and processes. Writtenin the highly successful Methods in Molecular Biology series format,chapters include introduction to their respective topics, lists of thenecessary material and reagents, step-by-step, readily reproducible laboratoryprotocols, and tips on troubleshooting and avoiding known pitfalls.

Thoroughand cutting-edge, Bacterial Cell Wall Homeostasis: Methods and Protocolsemphasizes the diversity of the research taking place in bacterial cell wallhomeostasis, and explains how the integration of information from acrossmultiple disciplines is going to be essential if a holistic understanding ofthis important process is to be obtained.

Produktdetails

• Methods in Molecular Biology 1440
• Verlag: Humana / Springer New York / Springer, Berlin
• Artikelnr. des Verlages: 978-1-4939-8114-4
• Softcover reprint of the original 1st ed. 2016
• Seitenzahl: 300
• Erscheinungstermin: 30. Mai 2018
• Englisch
• Abmessung: 254mm x 178mm x 17mm
• Gewicht: 574g
• ISBN-13: 9781493981144
• ISBN-10: 1493981145
• Artikelnr.: 52906186

Inhaltsangabe

AtomicForce Microscopic Analysis of Bacterial Cell Wall Peptidoglycan Architecture.- Ultra-SensitiveHigh Throughput Analytical Methods to Identify Novel Components Involved in CellWall Biology and to Study Bacterial Cell Wall Dynamics.- MicroarrayAnalysis to Monitor Bacterial Cell Wall Homeostasis.- Cell Shavingand False Positive Control Strategies Coupled to Novel Statistical Tools to ProfileGram-Positive Bacterial Surface Proteomes.- DifferentialProteomics Based on Multidimensional Protein Identification Technology to Understandthe Biogenesis of Outer Membrane of Escherichiacoli.- RandomTransposon Mutagenesis for Cell-Envelope Resistant to Phage Infection.- ZymographicTechniques for the Analysis of Bacterial Cell Wall in Bactillus.- Liquid Chromatography-Tandem Mass Spectrometryto Define Sortase Cleavage Products.- Geneticsand Cell Morphology Analyses of the Actinomycesoris srtA Mutant.- Constructionof a Bioassay System to Identify Extracellular Agents Targeting Bacterial Cell Envelope.- LuciferaseReporter Gene System to Detect Cell Wall Stress Stimulon Induction in Staphylococcus aureus.- Extractionand Analysis of Peptidoglycan Cell Wall Precursors.- ContinuousFluorescence Assay for Peptidoglycan Glycosyltransferases.- Analysisof Peptidoglycan Fragment Release.- Analysis of Cell Wall Teichoic Acids in Staphylococcus aureus.- Analysis of Bacterial Cell SurfaceChemical Composition Using Cryogenic X-Ray Photoelectron Spectroscopy.- Biophysical Measurements of BacterialCell Shape.- Coarse-Grained Molecular Dynamics Simulationsof the Bacterial Cell Wall.- StructuralComparison and Simulation of Pneumococcal Peptidoglycan Hydrolase LytB.