Animal Cell Culture Essential Methods

Ed. by John Davis

• Broschiertes Buch

Produktbeschreibung

This is a comprehensive research guide that describes both the key new techniques and more established methods. Every chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good practical advice, for immediate use at the bench. It presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. Detailed protocols for a wide variety of methods provide the core of each chapter, making new methodology easily accessible.
This book is an essential laboratory manual for all undergraduates and graduates about to embark on a cell culture project. It is a book which both experienced researchers and those new to the field will find invaluable.

Produktdetails

• Verlag: Wiley & Sons / Wiley-Blackwell
• Artikelnr. des Verlages: 14566658000
• 1. Auflage
• Seitenzahl: 384
• Erscheinungstermin: 21. März 2011
• Englisch
• Abmessung: 244mm x 170mm x 20mm
• Gewicht: 655g
• ISBN-13: 9780470666586
• ISBN-10: 0470666587
• Artikelnr.: 32568106

Autorenporträt

Dr John Davis: Visiting Lecturer in Biotechnology, University of Hertfordshire, previously Senior Project Scientist at Bio Products Laboratory, UK.

Inhaltsangabe

Contributors. Preface. Abbreviations. Protocols. 1 The Cell Culture
Laboratory (Sue Clarke and Janette Dillon). 2 Sterilization (Peter L.
Roberts). 3 Microscopy of Living Cells (Colin Gray and Daniel Zicha). 4
Basic Techniques and Media, the Maintenance of Cell Lines, and Safety (John
M. Davis). 5 Development and Optimization of Serum- and Protein-free
Culture Media (Stephen F. Gorfien and David W. Jayme). 6 Cryopreservation
and Banking of Cell Lines (Glyn N. Stacey, Ross Hawkins and Roland A.
Fleck). 7 Primary Culture of Specific Cell Types and the Establishment of
Cell Lines (Kee Woei Ng, Mohan Chothirakottu Abraham, David Tai Wei Leong,
Chris Morris and Jan-Thorsten Schantz). 8 Cloning (John Clarke, Alison
Porter and John M. Davis). 9 The Quality Control of Animal Cell Lines and
the Prevention, Detection and Cure of Contamination (Peter Thraves and
Cathy Rowe). 10 Systems for Cell Culture Scale-up (Jennifer Halsall and
John M. Davis). 11 Good Laboratory Practice in the Cell Culture Laboratory
(Barbara Orton). Index. Colour plates.