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Microbial fuel has showed a lot of promise in solving the three key sustainability issues the world is currently grappling with: energy security, global warming, and wastewater treatment. It is regarded as a clean energy replacement. Improvements and alterations have been performed to boost the power output and reach the goal. This book provides a concise explanation of the fundamentals of the Microbial Fuel Cell (MFC), including its operation, the use of various bacteria, chemical reaction details at the cathode and anode, distinct MFC types, features for heavy metal removal, and its many applications.