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A comparative pharmacognostical study between Acacia arabica and Prosopis julifera conclude that Acacia arabica plays more significant role and has more scientific value. Plants Acacia Arabica and Prosopis julifera were studies for pharmacognostical characteristic, morphology, microscopy, physicochemical, parameters which can be of utilized in identification and Authentication of plants. Successive, extractive and phytochemical screening revealed the present of Tannin Alkaloids, steroids and Terpenoids in various extracts ,however most of the medicinally potential phytoconstituents where present in alcoholic and aqueous extracts and Methnolic extracts used for the HPTLC analysis.