The first edition of Modern Size-Exclusion Liquid Chromatography
was published in 1979. A significant amount of advancement has
occurred in this field over the last 20 years, and the field of SEC
and its industrial and research applications has grown as well. (It
is widely used in industry.) As one of the reviewers points out,
this book was one of the best SEC books written at the time of its
publication and is still one of the best unified books on practice
and theory, despite being 20 years old. Sales of the first edition
(4,638 copies) and continued yearly sales, despite the book's
age, support this reviewer's claim.
The Second Edition of Modern Size-Exclusion Chromatography offers a
complete guide to the theories, methods, and applications of
size-exclusion chromatography. It provides an unparalleled,
integrated, up-to-date treatment of gel permeation and gel
filtration chromatography. With its detailed descriptions of
techniques, data handling, compilations of information on columns
and column packings, and tables of important solvents and reference
materials, the book offers readers everything they need to take
full advantage of this popular macromolecular characterization
technique.
Since publication of the first edition in 1979, there have been
many important advances in the field of size-exclusion
chromatography. This Second Edition brings the book thoroughly up
to date, with expert coverage of:
- New and emerging industrial and research applications
- Practical aspects of size-exclusion chromatography (SEC) and
multidetector and multidimensional SEC technologies for polymer
architecture and copolymer analysis
- Updated information on the latest equipment and techniques
- New best practices for the lab
- SEC in relation to polymer characterization techniques such as
GPEC, LCCC, and rheology
Throughout the text, detailed examples guide you step by step
through all the latest techniques and applications. With its
extensive revisions and updates written by leading experts and
pioneers in the field, Modern Size-Exclusion Liquid Chromatography
remains the definitive resource for the broad range of researchers
and scientists who use HPLC and GPC methods.
Ausstattung/Bilder: 2. Auflage. 2009. 494 S. 238 mm
Seitenzahl: 494
Best.Nr. des Verlages: 14620172000
Englisch
Abmessung: 243mm x 162mm x 29mm
Gewicht: 807g
ISBN-13: 9780471201724
ISBN-10: 0471201723
Best.Nr.: 25932582
"The authors have balanced well-written text with diagrams, figures, and equations to provide a user-friendly guide to practical modern SEC. The inclusion of both classical and current references with each chapter demonstrates the utility of each chapter and discussion. This text would be useful for scientists at any level, whether using SEC for the first time or the 1,000th." (Anal Bioanal Chem, 2011) "With its extensive revisions and updates written by leading experts and pioneers in the field, Modern Size-Exclusion Liquid Chromatography offers readers everything they need to take full advantage of this popular macromolecular characterisation technique." (Chemistry Journals, 11 April 2011)
André M. Striegel, PhD, is Assistant Professor in the Department of Chemistry & Biochemistry at the Florida State University, with previous industrial R&D experience at Solutia. He has published and lectured extensively in the area of polymer chromatography and is the recipient of several national awards in analytical chemistry. WALLACE W. YAU, PhD, received his doctorate in physical chemistry from University of Massachusetts and is currently a scientist specialist at Dow Chemical Co., with past industrial experience at DuPont and Chevron. He has been in GPC research and polymer characterization for the past four decades. JOSEPH J. KIRKLAND, PhD, is author or coauthor of eight widely read books on liquid chromatography, more than 150 peer-reviewed papers, and thirty patents. With over fifty years of industrial experience, he has been the recipient of many major national and international awards in analytical chemistry. DONALD D. BLY, PhD, retired as manager of analytical science, central research, DuPont. He has remained working as a consultant to analytical and analytical-polymer science businesses and has remained active in the American Chemical Society and ASTM.
Inhaltsangabe
1. BACKGROUND
1.1 Introduction
1.2 History
1.3 Utility of SEC
1.4eMolar Mass Averages and Molar Mass Distribution
1.5 Structure of This Book
2. RETENTION
2.1 Introduction
2.2 Solute Retention in LC
2.3 Solute Retention in SEC
2.4 SEC Retention Mechanism
2.5 Theoretical Models of SEC Separation
2.6 Other Considerations
3. BAND BROADENING
3.1 Introduction
3.2 LC Plate Theory
3.3 Mechanism of SEC Band Broadening
3.4 Influencing Factors
3.5 Experimental Methods
4. RESOLUTION
4.1 Introduction
4.2 Resolution Concept in SEC of Polymers
4.3 Molar Mass Accuracy Criterion
4.4 Applications of Column Performance Criteria
4.5 Pore Geometry and Operational Effects
5. EQUIPMENT
5.1 Introduction
5.2 Extracolumn Effects - General
5.3 Mobile-Phase Reservoirs, Inlet Filters, and Degassers
5.4 Solvent-Metering Systems (Pumps)
5.5 Sample Injectors and Autosamplers
5.6 Miscellaneous Hardware
5.7 Laboratory Safety
6. THE COLUMN
6.1 Introduction
6.2 Column Packings
6.3 Column Packing Methods
6.4 Column Performance
7. EXPERIMENTAL VARIABLES AND TECHNIQUES
7.1 Introduction
7.2 Solvent Effects
7.3 Substrate Effects
7.4 Sample Effects
7.5 Laboratory Techniques
7.6 Solvent Selection and Preparation
7.7 Selection and Use of Standard Reference Materials
7.8 Detector Selection
7.9 Column Selection and Handling
7.10 Chromatographic Design Considerations
7.11 Making the Separation
7.12 Troubleshooting
8. CALIBRATION
8.1 Introduction
8.2 Calibration with Narrow-MMD Standards
8.3 Calibration with Broad-MMD Standards
8.4 Accuracy of Calibration Methods
8.5 Actual Molar Mass Across the SEC Elution Curve
8.6 Linear Calibration Ranges
8.7 Recent Developments and Recommendations on Band-Broadening Correction (BBC)
