Protein Targeting Protocols - Giezen, Mark van der (ed.)
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In this updated volume, experts from around the world provide the latest protocols for isolating different organelles and the localization of particular proteins using a variety of methods such as light, confocal, and electron microscopy. Emphasis is placed on protein targeting of cellular compartments in both prokaryotic and eukaryotic systems. The book includes targeting protocols from different systems.…mehr

Produktbeschreibung
In this updated volume, experts from around the world provide the latest protocols for isolating different organelles and the localization of particular proteins using a variety of methods such as light, confocal, and electron microscopy. Emphasis is placed on protein targeting of cellular compartments in both prokaryotic and eukaryotic systems. The book includes targeting protocols from different systems.
  • Produktdetails
  • Methods in Molecular Biology Vol.390
  • Verlag: Springer, Berlin; Humana Press
  • Artikelnr. des Verlages: 12004591
  • 2nd ed.
  • Erscheinungstermin: Juni 2007
  • Englisch
  • Abmessung: 241mm x 160mm x 32mm
  • Gewicht: 939g
  • ISBN-13: 9781588297020
  • ISBN-10: 1588297020
  • Artikelnr.: 22696010
Autorenporträt
Mark van der Giezen, Queen Mary University of London, UK
Inhaltsangabe
Contents
Preface
Contributors
Bacterial systems
1 Localisation and mobility of bacterial proteins by confocal microscopy and fluorescence recovery after photobleaching (FRAP)
Conrad Mullineaux
2 Membrane protein insertion and secretion in bacteria
Jeanine de Keyzer, Martin van der Laan and Arnold J.M. Driessen
3 Identification of autotransporter proteins secreted by type V secretion systems in Gram-negative bacteria
Yihfen T. Yen and Christos Stathopoulos 4 Periplasmic targeting of recombinant proteins in Escherichia coli
Filipe J. M. Mergulhão and Gabriel A. Monteiro
5 In vitro analysis of the bacterial twin-arginine-dependent protein export
Michael Moser, Sascha Panahandeh, Eva Holzapfel and Matthias Müller
Eukaryotic systems
6 Nuclear import properties of the sex determining factor SRY
Jade K. Forwood, Gurpreet Kaur and David A. Jans
7 The mitochondrial machinery for import of precursor protein, a review.
Kip Gabriel and Nikolaus Pfanner
8 A genetic method to identify mitochondrial proteins in living mammalian cells
Takeaki Ozawa and Yoshio Umezawa
9 In vitro and in vivo methods to study protein import into plant mitochondria
Shashi Bhushan, Pavel F. Pavlov, Charlotta Rudhe and Elzbieta Glaser
10 Protein targeting to mitochondria of Saccharomyces cerevisiae and Neurospora crassa; in vitro and in vivo studies
Panagiotis Paptheodorou, Gra_nya Doma_ska and Joachim Rassow
11 A mitosome purification protocol based on Percoll density gradients and its use in validating the mitosomal nature of Entamoeba histolytica mitochondrial Hsp70
Jorge Tovar, Siân S.E. Cox and Mark van der Giezen
12 The chloroplast protein import machinery, a review
Friederike Hörmann, Jürgen Soll and Bettina Bölter
13 Import of plastid precursor proteins into pea chloroplasts
Jürgen Soll and Bettina Bölter
14Protein targeting in 'secondary' or 'complex' chloroplasts
Balbir K. Chaal and Beverley R. Green
15 Protein trafficking to the complex chloroplasts of Euglena
Rostislav Vacula, Silvia Sláviková and Steven D. Schwartzbach
16 Fluorescent protein fusions for protein localization in plants
John Runions, Chris Hawes and Smita Kurup
17 Genetic transformation; a tool to study protein targeting in diatoms
Peter Kroth
18 Identification of proteins targeted into the endoplasmic reticulum by cDNA library screening
Takeaki Ozawa and Yoshio Umezawa
19 Trafficking through the early secretory pathway of mammalian cells
Theresa H. Ward
20 Studying protein export from the endoplasmic reticulum in plants
Sally L. Hanton, Loren A. Matheson and Federica Brandizzi
21 Imaging the Golgi apparatus in living mitotic cells
Nihal Altan-Bonnet
22 Evaluating yeast biosynthetic vacuolar transport
Brian A. Davies, Darren S. carney and Bruce F. Horazdovsky
23 Targeting to lysosomes in mammalian cells: the biosynthetic and endocytic pathways
Ann H. Erickson and Jeffrey P. Bocock
24 Monitoring autophagy in yeast: the Pho8_60 assay
Daniel J. Klionsky
25 Protein targeting to yeast peroxisomes
Ida van der Klei and Marten Veenhuis
26 Expression, localization and topology of fluorescently tagged plasma membrane-targeted transmembrane proteins
Jeanne Shepshelovich and Koty Hirschberg
General methods
27 Intracellular protein localization by immunoelectron microscopy
Tetsuaki Osafune and Steven D. Schwartzbach
28 Separation of proteins by blue native electrophoresis (BN-PAGE)
Olga Randelj, Joachim Rassow and Christian Motz
29 Computational prediction of subcellular localization
Kenta Nakai and Paul Horton
30 Phylogenetic analysis to uncover organellar origins of nuclear-encoded genes
Bernardo Foth