Polyamines
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Recently, important new findings in the polyamine field and a variety of new experimental systems have revolutionized the study of these ubiquitous cellular components, essential for normal growth and development.  In Polyamines: Methods and Protocols , leading researchers contribute an extensive collection of up-to-date laboratory techniques for the further pursuit of polyamine study.  The volume delves into vital subjects such as neoplasia studies with animal models and human patients, therapeutic roles for polyamine inhibitors and analogs, polyamine metabolism and oxidative damage,…mehr

Produktbeschreibung
Recently, important new findings in the polyamine field and a variety of new experimental systems have revolutionized the study of these ubiquitous cellular components, essential for normal growth and development.  In Polyamines: Methods and Protocols, leading researchers contribute an extensive collection of up-to-date laboratory techniques for the further pursuit of polyamine study.  The volume delves into vital subjects such as neoplasia studies with animal models and human patients, therapeutic roles for polyamine inhibitors and analogs, polyamine metabolism and oxidative damage, polyamines as regulators of critical ion channels, as well as polyamine transport systems and polyamine-responsive genes.  Written in the highly successful Methods in Molecular Biology(TM) series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and expert notes on troubleshooting and avoiding known pitfalls.

 

Comprehensive and cutting-edge, Polyamines: Methods and Protocols provides a key resource for all scientists pursuing the study of this dynamic and significant aspect of cellular biology.
  • Produktdetails
  • Methods in Molecular Biology Vol.720
  • Verlag: Springer, Berlin; Humana Press
  • Artikelnr. des Verlages: 12599615
  • Seitenzahl: 505
  • Erscheinungstermin: April 2011
  • Englisch
  • Abmessung: 266mm x 187mm x 42mm
  • Gewicht: 1221g
  • ISBN-13: 9781617790331
  • ISBN-10: 1617790338
  • Artikelnr.: 32174724
Inhaltsangabe
Part I: Introduction 1. Current Status of the Polyamine Research Field Anthony E. Pegg and Robert A. Casero, Jr. Part II: Identification of Polyamine Metabolic Enzymes and Characterization of Polyamine-Regulated Genes and Ion Channels 2. Exploring Polyamine Biosynthetic Diversity through Comparative and Functional Genomics Anthony J. Michael 3. Characterization of Genes for Polyamine Modulon Kazuei Igarashi and Keiko Kashiwagi 4. Posttranscriptional Regulation of Gene Expression in Epithelial Cells by Polyamines Lan Xiao and Jian-Ying Wang 5. Identification, Chemical Synthesis, and Biological Functions of Unusual Polyamines Produced by Extreme Thermophiles Tairo Oshima, Toshiyuki Moriya, and Yusuke Terui 6. Polyamine Block of Inwardly-Rectifying Potassium Channels Harley T. Kurata, Wayland W.L. Cheng, and Colin G. Nichols Part III: Transgenic Models for Study of Polyamine Function 7. Carcinogenesis Studies in Mice with Genetically Engineered Alterations in Polyamine Metabolism David J. Feith 8. Transgenic Rodents with Altered SSAT Expression as Models of Pancreatitis and Altered Glucose and Lipid Metabolism Anne Uimari, Mervi T. Hyvönen, Eija Pirinen, and Leena Alhonen 9. Use of (Gyro) Gy and Spermine Synthase Transgenic Mice to Study Functions of Spermine Xiaojing Wang and Anthony E. Pegg Part IV: Enzymes Involved in Polyamine Catabolism and Post-Translational Protein Modification 10. A Simple Assay for Mammalian Spermine Oxidase: A Polyamine Catabolic Enzyme Implicated in Drug Response and Disease Andrew C. Goodwin, Tracy R. Murray-Stewart, and Robert A. Casero, Jr. 11. Characterization, Assay, and Substrate Specificity of Plant Polyamine Oxidases Panagiotis N. Moschou and Kalliopi A. Roubelakis-Angelakis 12. Assay of Deoxyhypusine Synthase Activity Edith C. Wolff, Seung Bum Lee, and Myung Hee Park 13. Assay of Deoxyhypusine Hydroxylase Activity Jong Hwan Park, Edith C. Wolff, and Myung Hee Park Part V: Regulation of Polyamine Content 14. Identification and Assay of Allosteric Regulators of S-Adenosylmethionine Decarboxylase Erin K. Willert, Lisa N. Kinch, and Margaret A. Phillips 15. Protocols for Studying Antizyme Expression and Function Noriyuki Murai, Yasuko Murakami, and Senya Matsufuji 16. Identification, Assay, and Functional Analysis of the Antizyme Inhibitor Family Chaim Kahana 17. Post-Transcriptional Regulation of Ornithine Decarboxylase Shannon L. Nowotarski, Sofia Origanti, and Lisa M. Shantz Part VI: Polyamine Transport and Uptake 18. Identification and Assays of Polyamine Transport Systems in Escherichia coli and Saccharomyces cerevisiae Keiko Kashiwagi and Kazuei Igarashi 19. Genetic and Biochemical Analysis of Protozoal Polyamine Transporters Marie-Pierre Hasne and Buddy Ullman 20. Heparan Sulfate Proteoglycan-Mediated Polyamine Uptake Johanna Welch, Katrin Svensson, Paulina Kucharzewska, and Mattias Belting 21. Polyamine Transport Systems in Mammalian Cells and Tissues Takeshi Uemura and Eugene W. Gerner 22. Procedures to Evaluate the Importance of Dietary Polyamines Paul Acheampong, Mary J. Macleod, and Heather M. Wallace Part VII: Polyamines and Disease 23. Determination of N 1 , N 12 -Diacetylspermine in Urine: A Novel Tumor Marker Masao Kawakita, Kyoko Hiramatsu, Mari Yanagiya, Yosuke Doi, and Mieko Kosaka 24. Spermidine/spermine- N 1 -Acetyltransferase in Kidney Ischemia Reperfusion Injury Kamyar Zahedi and Manoocher Soleimani 25. Use of Polyamine Metabolites as Markers for Stroke and Renal Failure Kazuei Igarashi and Keiko Kashiwagi 26. Methods to Evaluate Alterations in Polyamine Metabolism Caused by Helicobacter pylori Infection Alain P. Gobert, Rupesh Chaturvedi, and Keith T. Wilson 27. High-Resolution Capillary Gas Chromatography in Combination with Mass Spectrometry for Quantification of Three Major Polyamines in Post-Mor