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I started insect cell culture work in 1962, when T. D. C. Grace reported the first establishment of invertebrate continuous cell lines. He obtained grow ing cells from pupal ovaries of the emperor gum moth, Antheraea euca lypti. At that time, I was trying to obtain growing cells from leafhoppers. Grace's method could not be applied directly to my culture because of the differences in species, the size of the insects, and the tissue to be cul tured. The vertebrate tissue culture methods gave me some ideas for pre paring cultures from leafhoppers, but those could not be used directly either.…mehr

Produktbeschreibung
I started insect cell culture work in 1962, when T. D. C. Grace reported the first establishment of invertebrate continuous cell lines. He obtained grow ing cells from pupal ovaries of the emperor gum moth, Antheraea euca lypti. At that time, I was trying to obtain growing cells from leafhoppers. Grace's method could not be applied directly to my culture because of the differences in species, the size of the insects, and the tissue to be cul tured. The vertebrate tissue culture methods gave me some ideas for pre paring cultures from leafhoppers, but those could not be used directly either. There were no textbooks and no manuals for invertebrate tissue culture, so I had to develop a method by myself. First, I considered what type and what size of vessels are suitable for insect tissue culture. Also, I had to look for suitable materials to construct the culture vessels. Sec ond, I had to examine various culture media, especially growth-promot ing substances, such as sera. Then I had to improve culture media by trial and error. The procedure to set up a primary culture was also a problem. How could I sterilize materials? How could I remove tissues from a tiny insect? How many tissues should I pool in order to set up one culture? I had to find out the answers. Naturally, it took a lot of time.
  • Produktdetails
  • Springer Lab Manuals
  • Verlag: Springer, Berlin
  • Softcover reprint of the original 1st ed. 2002
  • Seitenzahl: 464
  • Erscheinungstermin: 1. Februar 2002
  • Englisch
  • Abmessung: 235mm x 155mm x 24mm
  • Gewicht: 740g
  • ISBN-13: 9784431703136
  • ISBN-10: 4431703136
  • Artikelnr.: 24991601
Autorenporträt
Jun Mitsuhashi, Tokyo University of Agriculture, Tokyo, Japan
Inhaltsangabe
Preface
Introduction
Part I. General Methods
Chapter 1. Facilities and Equipment
Chapter 2. Basic Information and Overview
Chapter 3. Preparation of Media
Chapter 4. General Cell Culture Methods
Part II. Methods for Setting Up Primary Cultures Specific to Animal Groups
Chapter 5. Insecta (Lepidoptera)
Chapter 6. Insecta (Diptera)
Chapter 7. Insecta (Coleoptera)
Chapter 8. Insecta (Hemiptera)
Chapter 9. Insecta (Hymenoptera)
Chapter 10. Insecta (Blattaria)
Chapter 11. Insecta (Orthoptera)
Chapter 12. Arthropoda Other than Insecta
Chapter 13. Prochordata
Chapter 14. Echinodermata
Chapter 15. Mollusca
Chapter 16. Annelida
Chapter 17. Nematoda
Chapter 18. Plathelminthes
Chapter 19. Coelenterata
Chapter 20. Porifera
Part III. Organ cultures
Chapter 21. Insecta
Chapter 22. Arthropoda other than Insecta
Chapter 23. Prochordata
Chapter 24. Echinodermata
Chapter 25. Mollusca
Chapter 26. Annelida
Chapter 27. Nematoda
Chapter 28. Plathelminthes
Chapter 29. Coelenterata
Chapter 30. Porifera
Part IV. Related Techniques
Chapter 30. Cell Cloning
Chapter 32. Karyotype Analyses
Chapter 33. Identification of Cell Lines
Chapter 34. Viability of Cells
Chapter 35. Viable Cell Enumeration
Chapter 36. Growth Rate
Chapter 37. Microscope Photography
Chapter 38. Virus Inoculation-Plaque Assay
Chapter 39. Examination of Drug Effects
Chapter 40. Cell Fusion
Chapter 41. Gene technology
Chapter 42. Large Scale Cell Cultures
I. Composition of Salt Solutions and Culture Meda
A. Salt Solutions
B. Culture Media
II. List of Invertebrate Continuous Cell Lines Reported
III. Commercial Products of Media and List of Suppliers
A. Commercial Products of Media
B. List of Suppliers
Index.