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With this valuable practical guide, three members of the HarvardStem Cell Institute have compiled and edited the definite handbookfor the exciting new field of human embryonic stem cell research.The editors have gathered protocols from scientists with extensivereputation and expertise, describing and comparing currently usedtechniques for the culture of human stem cells and discussing thestrengths and weaknesses of the different approaches. Human Embryonic Stem Cells: The PracticalHandbook contains the first centralised collection ofmethods used in human embryonic stem cell biology. The book…mehr

Produktbeschreibung
With this valuable practical guide, three members of the HarvardStem Cell Institute have compiled and edited the definite handbookfor the exciting new field of human embryonic stem cell research.The editors have gathered protocols from scientists with extensivereputation and expertise, describing and comparing currently usedtechniques for the culture of human stem cells and discussing thestrengths and weaknesses of the different approaches. Human Embryonic Stem Cells: The PracticalHandbook contains the first centralised collection ofmethods used in human embryonic stem cell biology. The book coversthe derivation of human stem cell lines, the obtaining of cellsfrom human stem cell banks, the culturing and characterisation ofthe cells, and the differentiation of the cells in vitro andin vivo. Lastly, almost all of these protocols can also beused for analyzing and manipulating induced pluripotency iPS stemcells. This allows an even greater number of opportunities forthose interested in purusing work in pluripotent stem cells,disease modelling, and other aspects of basic regenerative medicineresearch. The novel and useful focus of this book sets it apart from otheravailable books: * Compares and evaluates the protocols used in leadinglaboratories working on human embryonic stem cells * Centred solely on practical protocols for human (not mouse)embryonic stem cell research * Includes extensive troubleshooting sections * Addresses the different proclivities and behaviours ofindividual human embryonic cell lines * Contains techniques currently known only to a small number ofspecialised laboratories worldwide This handbook represents an essential source of up-to-datepractical information for all cell and developmental biologistsworking with human embryonic stem cells or wishing to enter thefield. It is also essential reading for clinical researchersin areas such as diabetes, cardiovascular disease, and neurologicaldiseases. Praise from the reviews: "...a highly readable and useful book... A notablefeature of the book is its air of openness and honesty... Thisbook... will help many to navigate the uncharted waters ofhuman embryonic stem cell biology." BRITISH SOCIETY FOR CELLBIOLOGY "... the imaginative solutions in this book can inspire us toget past our most frustrating limitations." CELL STEM CELL "... the richness in the details of each protocol presentedwill certainly encourage more scientists to begin studies of Humanpluripotent stem cells..." REGENERATIVE MEDICINE "In this fast-moving field, this [handbook] will help driveadvances of more and more researchers." DIFFERENTIATION "...a valuable resource for seasoned and noviceresearchers... an excellent addition to the reference collection ofany medical library or research laboratory." THE AMERICANMEDICAL ASSOCIATION

Dieser Download kann aus rechtlichen Gründen nur mit Rechnungsadresse in A, D ausgeliefert werden.

  • Produktdetails
  • Verlag: John Wiley & Sons
  • Seitenzahl: 424
  • Erscheinungstermin: 02.08.2008
  • Englisch
  • ISBN-13: 9780470511596
  • Artikelnr.: 37293952
Autorenporträt
Stephen Sullivan. Harvard University, Cambridge, MA, USA
Kevin Eggan. Harvard University, Cambridge, MA, USA
Chad A. Cowan. Harvard University, Cambridge, MA, USA
Inhaltsangabe
Foreword. Davor Solter

Preface.

Section 1: Obtaining and culturing human embryonic stem cells.

1 Organization and good aseptic technique in the human embryonic stem cell laboratory.
Minal J Patel, Emma L Stephenson, and Stephen L Minger

2 Sourcing human embryonic stem cell lines.
Glyn Stacey

3 Human embryo culture for human embryonic stem cell derivation.
R. Douglas Powers and Jeannine Witmyer

4 Derivation of human embryonic stem cell lines.
Stephen Sullivan, Dieter Egli, Hidenori Akutsu, Douglas A. Melton, Kevin Eggan and Chad A. Cowan

5 Standard culture of human embryonic stem cells.
Jeremy M. Crook, Rachel Horne, and Alan Colman

6 Culture of human embryonic stem cells in chemically defined conditions.
Julie Hsu Clark and Sheng Ding

Section 2: Characterization of undifferentiated embryonic stem cells.

7 Phenotypic analysis of human embryonic stem cells.
Jonathan S. Draper, Cheryle A. Séguin and Peter W. Andrews

8 Genetic and epigenetic analysis of human embryonic stem cells.
Laurie A. Boyer, Rudolf Jaenisch, and Maisam Mitalipova

Section 3: Manipulation of human embryonic stem cells.

9 In vivo differentiation of human embryonic stem cells.
Scott A. Noggle, Francesca M. Spagnoli, Ali H. Brivanlou

10 In vitro differentiation of human embryonic stem cells.
A. Henry Sathananthan and Alan Trounson

11 Differentiation of human embryonic stem cells into extraembryonic cell types.
Cheryle A. Séguin and Jonathan S. Draper

12 Directed differentiation of human embryonic stem cells into:
(a) Early endoderm cells.
Kenji Osafune, Alice E. Chen and Douglas A. Melton
(b) Hepatic cells.
Neta Lavon and Nissim Benvenisty
(c) Pancreatic cells.
Hiram Chipperfield

13 Directed differentiation of human embryonic stem cells into:
(a) Cardiomyocytes.
Christine Mummery, Robert Passier and Chris Denning
(b) Endothelial cells.
Carrie Soukup, Shulamit Levenberg and Ondine Cleaver
(c) Osteogenic cells.
Jeffrey M. Karp, Alborz Mahdavi, Lino S. Ferreira, Ali Khademhosseini and Robert Langer
(d) Hematopoietic in vivo repopulating cells.
Shannon McKinney-Freeman, Thorsten M. Schlaeger and George Q. Daley
(e) Lymphocytes.
Petter S. Woll and Dan S. Kaufman
(f) Myeloid cells.
Chantal Cerdan and Mickie Bhatia

14 Directed differentiation of human embryonic stem cells into:
(a) Forebrain neurons.
Emily A. Davis and Lawrence S.B. Goldstein
(b) Dopaminergic neuron.
Jan Pruszak and Ole Isacson
(c) Spinal motor neurons.
Bao-Yang Hu and Su-Chun Zhang

15 (a) Gene targeting: knock out and knock in by homologous recombination.
Thomas P. Zwaka
(b) RNA interference in human embryonic stem cells.
M. William Lensch, Asmin Tulpule and Holm Zaehres
(c) Generation of gene reporters using bacterial artificial chromosome recombineering. Andrew J. Washkowitz and David A. Shaywitz

Afterword. Azim Surani

Index.
Rezensionen
"...the imaginative solutions in this book can inspire us to get past our most frustrating limitations."
Cell Stem Cell"...the richness in the details of each protocol presented will certainly encourage more scientists to begin studies of Human ES cells..."
Future Medicine "In this fast-moving field, this [handbook] will help drive advances of more and more researchers." Differentiation"... the richness in the details of each protocol presented will certainly encourage more scientists to begin studies of Human pluripotent stem cells..." REGENERATIVE MEDICINE"......a valuable resource for seasoned and novice researchers. ...an excellent addition to any...medical library or research laboratory."
THE AMERICAN MEDICAL ASSOCIATION