Schnelle und einfache Lösungen für HPLC-Probleme!Antworten auf 45 typische Fragen von HPLC-Anwendern und allgemeinen Schlußfolgerungen werden auf nicht mehr als jeweils vier Seiten dargestellt. Ein Erste-Hilfe-Set für jeden HPLC-Anwender!Das Buch ist eine erweiterte englische Übersetzung des Titels 'HPLC-Tips'. Schnelle und einfache Lösungen für HPLC-Probleme!Antworten auf 45 typische Fragen von HPLC-Anwendern und allgemeinen Schlußfolgerungen werden auf nicht mehr als jeweils vier Seiten dargestellt. Der Inhalt umfaßt- einfache Gerätetests und Auswahlkriterien für Säulen, Pufferlösungen usw.-…mehr
Schnelle und einfache Lösungen für HPLC-Probleme!Antworten auf 45 typische Fragen von HPLC-Anwendern und allgemeinen Schlußfolgerungen werden auf nicht mehr als jeweils vier Seiten dargestellt. Ein Erste-Hilfe-Set für jeden HPLC-Anwender!Das Buch ist eine erweiterte englische Übersetzung des Titels 'HPLC-Tips'. Schnelle und einfache Lösungen für HPLC-Probleme!Antworten auf 45 typische Fragen von HPLC-Anwendern und allgemeinen Schlußfolgerungen werden auf nicht mehr als jeweils vier Seiten dargestellt. Der Inhalt umfaßt- einfache Gerätetests und Auswahlkriterien für Säulen, Pufferlösungen usw.- spezielle Probleme und ihre Lösung- Möglichkeiten zur Optimierung von TrennungenDas Buch enthält außerdem ein spezielles Kapitel über die Retention ionisierbarer Substanzen in der RP-HPLC, weiterführende Literatur, Tabellen mit nützlichen Daten sowie Checklisten. Es ist ein Erste-Hilfe-Set für jeden HPLC-Anwender!Das Buch ist eine erweiterte englische Übersetzung des Titels 'HPLC-Tips'.
Introduction SIMPLE TESTS AND DECISION CRITERIA Tip 01: What is in a name of a column material? Tip 02: Is this C18 column the right choice for my sample? Tip 03: Why are polar solutes well separated with one C18 column and barely with another? Tip 04: How can I clean the RP-phase fast? Tip 05: How do I best degas my mobile phase? Tip 06: Methanol or acetonitrile? Tip 07: The pH-value of the mobile phase is too high/too low - what to do? Tip 08: Which is the right ionic strength of the buffer? Tip 09: How to make sense of the dead volume of an isocratic equipment? Tip 10: Taking over a gradient method - the influence of the instrumentation Tip 11: Does the pump work correctly, precisely or accurately? Tip 12: How to test an HPLC equipment and its modules? Tip 13: Injection of solutes out of aqueous solutions Tip 14: Which is the largest tolerable injection volume? Tip 15: How critical are temperature changes? PartTip I: General comments, Detector Tip 16: How critical are temperature changes? Part TIP II: Column, Separation Tip 17: How to choose an HPLC equipment and a supplier? Tip 18: Is the current method a robust one? PROBLEMS AND THEIR SOLUTIONS Tip 19: Sample preparation - how critical are which mistakes? Tip 20: Flushing of an HPLC equipment Tip 21: Junk in the UV detection cell Tip 22: The lamp is new - what happened to the peak? Tip 23: What are the causes for pressure changes or deviations? Tip 24: Is the right or the left pump head defect? Tip 25: Baseline noise and damping Tip 26: The retention times increase - is it the pump or the mobile phase? Tip 27: Which buffer is right for which pH-value? Tip 28: An interesting alternative for the separation of acids and bases with buffer Tip 29: What can be the reasons for a change in retention times? Tip 30: I use up a lot of RP-columns, what should I do? Tip 31: Why does my normal phase system not work anymore? Tip 32: Chemical tailing at the presence of metal ions Tip 33: How to avoid memory effects? Tip 34: How do the default values on my PC affect the resolution? HINTS TO OPTIMIZE THE SEPARATION Tip 35: Which is the right injection technique to get sharper peaks? Tip 36: My peaks appear too early - how can I move them in an RP system to later retention times? Tip 37: How can I increase the plate number? Tip 38: Limit of detectiTip on: how can I see more? Tip 39: How can I speed up a separation? Tip 40: How can I optimize a separation? Tip 41: Dead volume, capacity factor, selectivity - how can I use them in every day life? Tip 42: Which flow is optimal for me? Tip 43: How can I optimize a gradient elution? Tip 44: Separation of ionic solutes: what works out best - endcapped phases, inert phases, phosphate buffer or ion pairing reagents? Part I Tip 45: Separation of ionic solutes: what works out best - endcapped phases, inert phases, phosphate buffer or ion pairing reagents? Part II RETENTION OF IONIZIBLE COMPONENTS IN REVERSED-PHASE HPLC Appendix
Introduction SIMPLE TESTS AND DECISION CRITERIA Tip 01: What is in a name of a column material? Tip 02: Is this C18 column the right choice for my sample? Tip 03: Why are polar solutes well separated with one C18 column and barely with another? Tip 04: How can I clean the RP-phase fast? Tip 05: How do I best degas my mobile phase? Tip 06: Methanol or acetonitrile? Tip 07: The pH-value of the mobile phase is too high/too low - what to do? Tip 08: Which is the right ionic strength of the buffer? Tip 09: How to make sense of the dead volume of an isocratic equipment? Tip 10: Taking over a gradient method - the influence of the instrumentation Tip 11: Does the pump work correctly, precisely or accurately? Tip 12: How to test an HPLC equipment and its modules? Tip 13: Injection of solutes out of aqueous solutions Tip 14: Which is the largest tolerable injection volume? Tip 15: How critical are temperature changes? PartTip I: General comments, Detector Tip 16: How critical are temperature changes? Part TIP II: Column, Separation Tip 17: How to choose an HPLC equipment and a supplier? Tip 18: Is the current method a robust one? PROBLEMS AND THEIR SOLUTIONS Tip 19: Sample preparation - how critical are which mistakes? Tip 20: Flushing of an HPLC equipment Tip 21: Junk in the UV detection cell Tip 22: The lamp is new - what happened to the peak? Tip 23: What are the causes for pressure changes or deviations? Tip 24: Is the right or the left pump head defect? Tip 25: Baseline noise and damping Tip 26: The retention times increase - is it the pump or the mobile phase? Tip 27: Which buffer is right for which pH-value? Tip 28: An interesting alternative for the separation of acids and bases with buffer Tip 29: What can be the reasons for a change in retention times? Tip 30: I use up a lot of RP-columns, what should I do? Tip 31: Why does my normal phase system not work anymore? Tip 32: Chemical tailing at the presence of metal ions Tip 33: How to avoid memory effects? Tip 34: How do the default values on my PC affect the resolution? HINTS TO OPTIMIZE THE SEPARATION Tip 35: Which is the right injection technique to get sharper peaks? Tip 36: My peaks appear too early - how can I move them in an RP system to later retention times? Tip 37: How can I increase the plate number? Tip 38: Limit of detectiTip on: how can I see more? Tip 39: How can I speed up a separation? Tip 40: How can I optimize a separation? Tip 41: Dead volume, capacity factor, selectivity - how can I use them in every day life? Tip 42: Which flow is optimal for me? Tip 43: How can I optimize a gradient elution? Tip 44: Separation of ionic solutes: what works out best - endcapped phases, inert phases, phosphate buffer or ion pairing reagents? Part I Tip 45: Separation of ionic solutes: what works out best - endcapped phases, inert phases, phosphate buffer or ion pairing reagents? Part II RETENTION OF IONIZIBLE COMPONENTS IN REVERSED-PHASE HPLC Appendix
Rezensionen
"This book is a good reference source for HPLC laboratory practice, for operators both novice and expert. It is written in an approachable manner, illustrated with clear tables and working examples, which should enable a novice to achieve good results and an experienced operator to become more efficient."
John F. Kennedy, Carbohydrate Polymers, 2004, Vol. 55
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