Proteomics

Introduction to Methods and Applications
Herausgegeben:Kraj, Agnieszka; Silberring, Jerzy

• Gebundenes Buch

Produktbeschreibung

Introduction to Proteomics is written by seasoned researchers with years of practical experience. In addition to comprehensive discussions of the basic concepts, techniques, and applications of the subject, the text also includes an extensive glossary and a chapter containing laboratory exercises and protocols.

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Everything you need to begin conducting your own proteomics research

Proteomics: Introduction to Methods and Applications is the ideal introduction to the field of proteomics, giving readers the skills and knowledge needed to study proteins, their structure, and their function in cells, tissues, and organisms. Written by experienced researchers, the text not only covers all the basic concepts, techniques, and applications, it also offers readers insights and practical tips based on the authors' many years of hands-on work in proteomics research. Moreover, the text has been carefully edited to ensure a thorough, consistent, and highly readable approach throughout all the chapters.

This text offers everything readers need to begin conducting their own proteomics research, including:
_ A full chapter dedicated to laboratory exercises and protocols to help readers apply their skills
_ Step-by-step explanations of key analytical methods, including mass spectrometry, sample preparation, chromatographic and electrophoretic separation, and micro-total analysis systems
_ Complete coverage of bioinformatics, including an introduction to important proteomics databases
_ A glossary defining the key terms in the field
_ An appendix with an annotated list of Internet resources, information on post-translational modifications, and the masses and compositions of amino acid residues

This text is designed for upper-level undergraduate and graduate courses in proteomics. Upon successful completion of the text, students are able to apply their skills and knowledge to solve problems in such fields as biotechnology, drug discovery, and medical research.

Produktdetails

• Wiley-Interscience Series on Mass Spectrometry
• Verlag: Wiley & Sons
• 1. Auflage
• Seitenzahl: 352
• Erscheinungstermin: 1. Oktober 2008
• Englisch
• Abmessung: 241mm x 158mm x 24mm
• Gewicht: 630g
• ISBN-13: 9780470055359
• ISBN-10: 0470055359
• Artikelnr.: 23819103

Autorenporträt

Agnieszka Kraj, PHD, is an Associate Professor of the Neurobiochemistry Department in the Faculty of Chemistry at Jagiellonian University, Poland. Jerzy Silberring, PHD, is the Head of the Neurobiochemistry Department in the Faculty of Chemistry as well as the former deputy head of the Regional Laboratory for Physicochemical Analyses at Jagiellonian University, Poland. Dr. Silberring is coeditor of Mass Spectrometry and Hyphenated Techniques in Neuropeptide Research and Mass Spectrometry: Instrumentation, Interpretation, and Applications (both from Wiley).

Inhaltsangabe

Foreword. 1. Introduction to Proteomics and Strategy for Proteins
Identification. 2. Sample Preparation. 3. Chromatographic Separation
Methods. 3.1. Liquid Chromatography Linked to Mass Spectrometry. 3.2.
Multidimensional Liquid Chromatography Combined with Mass Spectrometry. 4.
Electrophoretic Separation Methods. 4.1. One-Dimensional Gel
Electrophoresis. 4.2. Two-Dimensional Gel Electrophoresis. 5. Capillary
Electrophoresis. 6. Protein Blotting. 7. Introduction to Proteins and
Peptides Mass Spectrometry. 7.1. Electrospray Ionization (ESI). 7.2.
Matrix-Assisted Laser Desorption Ionization (MALDI). 7.3. Imaging Mass
Spectrometry. 7.4. Fragmentation and Sequencing in Proteomics. 7.5. Protein
Identification by Mass Spectrometry. 7.6. Chemical Derivatization for
Sequence Analysis. 8. Protein Modifications. 9. Microfluidics. 10.
Proteomics. 10.1. Quantitative Proteomics. 10.2. Structural Proteomics.
10.3. Functional Proteomics. 10.4. Clinical Proteomics. 11. Bioinformatics.
12. Appendix. 13. The Laboratory Practicals. 13.1. Polyacrylamide Gel
Electrophoresis under Denaturing Conditions. 13.2. Preparation of the
In-Gel Separated Proteins for Mass Spectrometry. 13.3. Bioinformatic Tools
for Protein Identification. 13.4. LC-MS and LC-MS/MS - Proteomics
Applications. 13.5. Enzyme Kinetics. 13.6. Internet Databases.