Proteomics of Biological Systems (eBook, ePUB)
Protein Phosphorylation Using Mass Spectrometry Techniques
Proteomics of Biological Systems (eBook, ePUB)
Protein Phosphorylation Using Mass Spectrometry Techniques
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Phosphorylation is the addition of a phosphate (PO4) group to a protein or other organic molecule. Phosphorylation activates or deactivates many protein enzymes, causing or preventing the mechanisms of diseases such as cancer and diabetes. This book shows how to use mass spectrometry to determine whether or not a protein has been correctly modified by the addition of a phosphate group. It also provides a combination of detailed, step-by-step methodology for phosphoproteomic sample preparation, mass spectral instrumental analysis, and data interpretation approaches. Furthermore, it includes the…mehr
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- Produktdetails
- Verlag: John Wiley & Sons
- Seitenzahl: 376
- Erscheinungstermin: 23. September 2011
- Englisch
- ISBN-13: 9781118137031
- Artikelnr.: 37345366
- Verlag: John Wiley & Sons
- Seitenzahl: 376
- Erscheinungstermin: 23. September 2011
- Englisch
- ISBN-13: 9781118137031
- Artikelnr.: 37345366
Modification (PTM) of Proteins 1 1.1 Over 200 Forms of PTM of Proteins 1
1.2 Three Main Types of PTM Studied by MS 2 1.3 Overview of
Nano-Electrospray/Nanofl ow LC-MS 2 1.3.1 Defi nition and Description of MS
2 1.3.2 Basic Design of Mass Analyzer Instrumentation 3 1.3.3 ESI 7 1.3.4
Nano-ESI 11 1.4 Overview of Nucleic Acids 15 1.5 Proteins and Proteomics 20
1.5.1 Introduction to Proteomics 20 1.5.2 Protein Structure and Chemistry
22 1.5.3 Bottom-Up Proteomics: MS of Peptides 27 1.5.4 Top-Down Proteomics:
MS of Intact Proteins 42 1.5.5 Systems Biology and Bioinformatics 48 1.5.6
Biomarkers in Cancer 52 Reference 56 2 Glycosylation of Proteins 59 2.1
Production of a Glycoprotein 59 2.2 Biological Processes of Protein
Glycosylation 59 2.3 N-Linked and O-Linked Glycosylation 60 2.4
Carbohydrates 60 2.4.1 Ionization of Oligosaccharides 64 2.4.2 Carbohydrate
Fragmentation 65 2.4.3 Complex Oligosaccharide Structural Elucidation 70
2.5 Three Objectives in Studying Glycoproteins 72 2.6 Glycosylation Study
Approaches 72 2.6.1 MS of Glycopeptides 73 2.6.2 Mass Pattern Recognition
75 2.6.3 Charge State Determination 76 2.6.4 Diagnostic Fragment Ions 76
2.6.5 High-Resolution/High-Mass Accuracy Measurement and Identification 76
2.6.6 Digested Bovine Fetuin 78 Reference 79 3 Sulfation of Proteins as
Posttranslational Modification 81 3.1 Glycosaminoglycan Sulfation 81 3.2
Cellular Processes Involved in Sulfation 81 3.3 Brief Example of
Phosphorylation 82 3.4 Sulfotransferase Class of Enzymes 82 3.5
Fragmentation Nomenclature for Carbohydrates 82 3.6 Sulfated Mucin
Oligosaccharides 83 3.7 Tyrosine Sulfation 84 3.8 Tyrosylprotein
Sulfotransferases TPST1 and TPST2 87 3.9 O-Sulfated Human Proteins 89 3.10
Sulfated Peptide Product Ion Spectra 89 3.11 Use of Higher Energy
Collisions 93 3.12 Electron Capture Dissociation (ECD) 94 3.13 Sulfation
versus Phosphorylation 95 Reference 97 4 Eukaryote PTM as Phosphorylation:
Normal State Studies 99 4.1 Mass Spectral Measurement with Examples of HeLa
Cell Phosphoproteome 99 4.1.1 Introduction 99 4.1.2 Protein Phosphatase and
Kinase 99 4.1.3 Hydroxy-Amino Acid Phosphorylation 100 4.1.4 Traditional
Phosphoproteomic Approaches 102 4.1.5 Current Approaches 103 4.1.6 The
Ideal Approach 107 4.1.7 One-Dimensional (1-D) Sodium Dodecyl Sulfate (SDS)
PAGE 108 4.1.8 Tandem MS Approach 108 4.1.9 Alternative Methods: Infrared
Multiphoton Dissociation (IRMPD) and Electron Capture Dissociation (ECD)
115 4.1.10 Electron Transfer Dissociation (ETD) 115 4.2 The HeLa Cell
Phosphoproteome 118 4.2.1 Introduction 118 4.2.2 Background of Study 118
4.2.3 What is Covered 119 4.2.4 Optimized Methods to Use for
Phosphoproteomic Studies 119 4.2.5 Description of Instrumental Analyses 123
4.2.6 Current Approaches for Peptide Identification and False Discovery
Rate (FDR) Determination 125 4.2.7 Results of the Protein Extraction and
Preparation 126 4.2.8 HeLa Cell Phosphoproteome Methodology Comparison 128
4.2.9 Overall Conclusion 134 4.3 Nonphosphoproteome HeLa Cell Analysis 135
4.3.1 IMAC Flow Through Peptide Analysis 135 4.3.2 IMAC NaCl Wash Peptide
Analysis 136 4.3.3 IMAC Flow Through versus NaCl Wash Comparison 138 4.3.4
Gene Ontology Comparison 138 4.3.5 IMAC Bed Nonspecifi c Binding Study 140
4.4 Reviewing Spectra Using the SpectrumLook Software Package 143 Reference
144 5 Eukaryote PTM as Phosphorylation: Perturbed State Studies 147 5.1
Study of the Phosphoproteome of HeLa Cells under Perturbed Conditions by
Nano-High-Performance Liquid Chromatography HPLC Electrospray Ionization
(ESI) Linear Ion Trap (LTQ)-FT/Mass Spectrometry (MS) 147 5.1.1
Introduction 147 5.1.2 Ataxia Telangiectasia Mutated (ATM) and ATM and
Rad3-Related (ATR) 149 5.1.3 Background of Study 149 5.1.4 Review of
Optimized Approach to Study 151 5.1.5 Phosphoproteome Gene Ontology (GO)
Comparison 160 5.1.6 Potential Regulated Target Proteins of PP5 162 5.1.7
GO Differential Comparison 167 5.1.8 Conclusion 175 5.1.9 Reviewing Spectra
Using the SpectrumLook Software Package 175 Reference 176 6 Prokaryotic
Phosphorylation of Serine, Threonine, and Tyrosine 181 6.1 Introduction 181
6.1.1 Serine (Ser)/Threonine (Thr)/Tyrosine (Tyr) Phosphorylation 181 6.1.2
Histidine (His) Phosphorylation 181 6.1.3 Caulobacter crescentus 181 6.1.4
Ser/Thr/Tyr Phosphorylation of C. crescentus 183 6.1.5 Ser/Thr/Tyr
Phosphorylation of Bacillus subtilis and Escherichia coli 184 6.1.6 C.
crescentus as Cell Cycle Model 185 6.1.7 Bacteria Starvation Response 187
6.1.8 First Coverage of C. crescentus Phosphoproteome 188 6.2 Optimized
Methodology for Phospho Ser/Thr/Tyr Studies 188 6.2.1 Bacterial Strain and
Growth Conditions 188 6.2.2 C. crescentus Cell Protein Extraction:
Phosphoproteomics 189 6.2.3 Solid-Phase Extraction (SPE) Desalting 190
6.2.4 In Vitro Methylation of Peptides 190 6.2.5 Phosphopeptide Enrichment
by IMAC 191 6.2.6 Normal Proteomics 192 6.2.7 pY Enrichment by IP 192 6.2.8
RP/Nano-High-Performance Liquid Chromatography (HPLC) Separation 192 6.2.9
LC-Linear Ion Trap (LTQ)-Orbitrap MS/MS 193 6.2.10 LTQ-Fourier Transform
(FT)/MS/MS 193 6.2.11 Peptide Identification and False Discovery Rate (FDR)
Determination 193 6.2.12 Peptide Quantitative Comparison 194 6.3 Identifi
cation of the Components of the Ser/Thr/Tyr Phosphoproteome in C.
crescentus Grown in the Presence and Absence of Glucose 194 6.3.1 Total
Phosphoprotein Identifications 194 6.3.2 MSA Spectra 196 6.3.3
Phosphorylation Sites Identifi ed 196 6.3.4 Ser/Thr/Tyr Phosphoproteome of
C. crescentus 205 6.3.5 Phosphorylated His and Aspartate 213 6.3.6 Cell
Cycle His Kinase CckA 215 6.3.7 Phosphoglutamate 216 6.3.8 Enriched Tyr
Phosphoproteome of C. crescentus 216 6.3.9 Carbon Environment-Shared
Phosphoproteome 217 6.3.10 Carbon-Rich versus Carbon-Starved Class/Category
225 6.3.11 Carbon-Rich versus Carbon-Starved Unique Phosphorylated Proteins
227 6.3.12 Confi rmation of Decreased Energy Pathways 232 6.3.13
Phosphopeptide Quantitative Differential Comparison 233 6.3.14 Carbon-Rich
versus Carbon-Starved Normal Proteome Time Course Study 235 6.3.15
Conclusions 243 6.3.16 Supplementary Material 243 Reference 244 7
Prokaryotic Phosphorylation of Histidine 249 7.1 Phosphohistidine as
Posttranslational Modification (PTM) 249 7.2 Bacterial Kinases and the
Two-Component System 250 7.3 Measurement of Phosphorylated His (pH) 251
7.3.1 Stabilities of Phosphorylated Amino Acids 251 7.3.2 Immobilized Metal
Affinity Chromatography (IMAC) and Mass Spectrometry (MS) 252 7.4 In Vitro
and In Vivo Study of pH-Containing Peptides by Nano-ESI Tandem MS 255 7.4.1
Introduction 255 7.4.2 Background of Study 257 7.4.3 Optimized Methodology
for Phosphohistidine Studies 259 7.4.4 C18 RP LC Behavior 268 7.4.5
Phosphohistidine Loses HPO3 and H3PO4 270 7.4.6 Q-TOF/MS/MS Product Ion
Spectra 277 7.4.7 Behavior of Monophosphohistidine and Diphosphohistidine
Peptide 281 7.4.8 Behavior of Phosphotyrosine and Phosphohistidine Peptide
285 7.4.9 Behavior of Phosphotyrosine-, Phosphothreonine-, and
Phosphohistidine-Containing Peptide 287 7.4.10 Validation of Cu(II)-Based
IMAC Phosphohistidine Enrichment 291 7.4.11 In Vivo Measurement of
Phosphohistidine 293 7.4.12 Gene Ontology of Phosphorylated Proteins 296
7.4.13 Predicted Regulatory Protein Motif Study 307 7.4.14 Validation of
Phosphohistidine-Containing Proteins 308 7.4.15 The pDpH Motif 310 7.4.16
Conclusions 311 7.5 Supplementary Material 311 7.5.1 Reviewing Spectra
Using the SpectrumLook Software Package 311 Reference 313 Appendix I Atomic
Weights and Isotopic Compositions 317 Appendix II Periodic Table of the
Elements 325 Appendix III Fundamental Physical Constants 327 Glossary 329
Index 345
Modification (PTM) of Proteins 1 1.1 Over 200 Forms of PTM of Proteins 1
1.2 Three Main Types of PTM Studied by MS 2 1.3 Overview of
Nano-Electrospray/Nanofl ow LC-MS 2 1.3.1 Defi nition and Description of MS
2 1.3.2 Basic Design of Mass Analyzer Instrumentation 3 1.3.3 ESI 7 1.3.4
Nano-ESI 11 1.4 Overview of Nucleic Acids 15 1.5 Proteins and Proteomics 20
1.5.1 Introduction to Proteomics 20 1.5.2 Protein Structure and Chemistry
22 1.5.3 Bottom-Up Proteomics: MS of Peptides 27 1.5.4 Top-Down Proteomics:
MS of Intact Proteins 42 1.5.5 Systems Biology and Bioinformatics 48 1.5.6
Biomarkers in Cancer 52 Reference 56 2 Glycosylation of Proteins 59 2.1
Production of a Glycoprotein 59 2.2 Biological Processes of Protein
Glycosylation 59 2.3 N-Linked and O-Linked Glycosylation 60 2.4
Carbohydrates 60 2.4.1 Ionization of Oligosaccharides 64 2.4.2 Carbohydrate
Fragmentation 65 2.4.3 Complex Oligosaccharide Structural Elucidation 70
2.5 Three Objectives in Studying Glycoproteins 72 2.6 Glycosylation Study
Approaches 72 2.6.1 MS of Glycopeptides 73 2.6.2 Mass Pattern Recognition
75 2.6.3 Charge State Determination 76 2.6.4 Diagnostic Fragment Ions 76
2.6.5 High-Resolution/High-Mass Accuracy Measurement and Identification 76
2.6.6 Digested Bovine Fetuin 78 Reference 79 3 Sulfation of Proteins as
Posttranslational Modification 81 3.1 Glycosaminoglycan Sulfation 81 3.2
Cellular Processes Involved in Sulfation 81 3.3 Brief Example of
Phosphorylation 82 3.4 Sulfotransferase Class of Enzymes 82 3.5
Fragmentation Nomenclature for Carbohydrates 82 3.6 Sulfated Mucin
Oligosaccharides 83 3.7 Tyrosine Sulfation 84 3.8 Tyrosylprotein
Sulfotransferases TPST1 and TPST2 87 3.9 O-Sulfated Human Proteins 89 3.10
Sulfated Peptide Product Ion Spectra 89 3.11 Use of Higher Energy
Collisions 93 3.12 Electron Capture Dissociation (ECD) 94 3.13 Sulfation
versus Phosphorylation 95 Reference 97 4 Eukaryote PTM as Phosphorylation:
Normal State Studies 99 4.1 Mass Spectral Measurement with Examples of HeLa
Cell Phosphoproteome 99 4.1.1 Introduction 99 4.1.2 Protein Phosphatase and
Kinase 99 4.1.3 Hydroxy-Amino Acid Phosphorylation 100 4.1.4 Traditional
Phosphoproteomic Approaches 102 4.1.5 Current Approaches 103 4.1.6 The
Ideal Approach 107 4.1.7 One-Dimensional (1-D) Sodium Dodecyl Sulfate (SDS)
PAGE 108 4.1.8 Tandem MS Approach 108 4.1.9 Alternative Methods: Infrared
Multiphoton Dissociation (IRMPD) and Electron Capture Dissociation (ECD)
115 4.1.10 Electron Transfer Dissociation (ETD) 115 4.2 The HeLa Cell
Phosphoproteome 118 4.2.1 Introduction 118 4.2.2 Background of Study 118
4.2.3 What is Covered 119 4.2.4 Optimized Methods to Use for
Phosphoproteomic Studies 119 4.2.5 Description of Instrumental Analyses 123
4.2.6 Current Approaches for Peptide Identification and False Discovery
Rate (FDR) Determination 125 4.2.7 Results of the Protein Extraction and
Preparation 126 4.2.8 HeLa Cell Phosphoproteome Methodology Comparison 128
4.2.9 Overall Conclusion 134 4.3 Nonphosphoproteome HeLa Cell Analysis 135
4.3.1 IMAC Flow Through Peptide Analysis 135 4.3.2 IMAC NaCl Wash Peptide
Analysis 136 4.3.3 IMAC Flow Through versus NaCl Wash Comparison 138 4.3.4
Gene Ontology Comparison 138 4.3.5 IMAC Bed Nonspecifi c Binding Study 140
4.4 Reviewing Spectra Using the SpectrumLook Software Package 143 Reference
144 5 Eukaryote PTM as Phosphorylation: Perturbed State Studies 147 5.1
Study of the Phosphoproteome of HeLa Cells under Perturbed Conditions by
Nano-High-Performance Liquid Chromatography HPLC Electrospray Ionization
(ESI) Linear Ion Trap (LTQ)-FT/Mass Spectrometry (MS) 147 5.1.1
Introduction 147 5.1.2 Ataxia Telangiectasia Mutated (ATM) and ATM and
Rad3-Related (ATR) 149 5.1.3 Background of Study 149 5.1.4 Review of
Optimized Approach to Study 151 5.1.5 Phosphoproteome Gene Ontology (GO)
Comparison 160 5.1.6 Potential Regulated Target Proteins of PP5 162 5.1.7
GO Differential Comparison 167 5.1.8 Conclusion 175 5.1.9 Reviewing Spectra
Using the SpectrumLook Software Package 175 Reference 176 6 Prokaryotic
Phosphorylation of Serine, Threonine, and Tyrosine 181 6.1 Introduction 181
6.1.1 Serine (Ser)/Threonine (Thr)/Tyrosine (Tyr) Phosphorylation 181 6.1.2
Histidine (His) Phosphorylation 181 6.1.3 Caulobacter crescentus 181 6.1.4
Ser/Thr/Tyr Phosphorylation of C. crescentus 183 6.1.5 Ser/Thr/Tyr
Phosphorylation of Bacillus subtilis and Escherichia coli 184 6.1.6 C.
crescentus as Cell Cycle Model 185 6.1.7 Bacteria Starvation Response 187
6.1.8 First Coverage of C. crescentus Phosphoproteome 188 6.2 Optimized
Methodology for Phospho Ser/Thr/Tyr Studies 188 6.2.1 Bacterial Strain and
Growth Conditions 188 6.2.2 C. crescentus Cell Protein Extraction:
Phosphoproteomics 189 6.2.3 Solid-Phase Extraction (SPE) Desalting 190
6.2.4 In Vitro Methylation of Peptides 190 6.2.5 Phosphopeptide Enrichment
by IMAC 191 6.2.6 Normal Proteomics 192 6.2.7 pY Enrichment by IP 192 6.2.8
RP/Nano-High-Performance Liquid Chromatography (HPLC) Separation 192 6.2.9
LC-Linear Ion Trap (LTQ)-Orbitrap MS/MS 193 6.2.10 LTQ-Fourier Transform
(FT)/MS/MS 193 6.2.11 Peptide Identification and False Discovery Rate (FDR)
Determination 193 6.2.12 Peptide Quantitative Comparison 194 6.3 Identifi
cation of the Components of the Ser/Thr/Tyr Phosphoproteome in C.
crescentus Grown in the Presence and Absence of Glucose 194 6.3.1 Total
Phosphoprotein Identifications 194 6.3.2 MSA Spectra 196 6.3.3
Phosphorylation Sites Identifi ed 196 6.3.4 Ser/Thr/Tyr Phosphoproteome of
C. crescentus 205 6.3.5 Phosphorylated His and Aspartate 213 6.3.6 Cell
Cycle His Kinase CckA 215 6.3.7 Phosphoglutamate 216 6.3.8 Enriched Tyr
Phosphoproteome of C. crescentus 216 6.3.9 Carbon Environment-Shared
Phosphoproteome 217 6.3.10 Carbon-Rich versus Carbon-Starved Class/Category
225 6.3.11 Carbon-Rich versus Carbon-Starved Unique Phosphorylated Proteins
227 6.3.12 Confi rmation of Decreased Energy Pathways 232 6.3.13
Phosphopeptide Quantitative Differential Comparison 233 6.3.14 Carbon-Rich
versus Carbon-Starved Normal Proteome Time Course Study 235 6.3.15
Conclusions 243 6.3.16 Supplementary Material 243 Reference 244 7
Prokaryotic Phosphorylation of Histidine 249 7.1 Phosphohistidine as
Posttranslational Modification (PTM) 249 7.2 Bacterial Kinases and the
Two-Component System 250 7.3 Measurement of Phosphorylated His (pH) 251
7.3.1 Stabilities of Phosphorylated Amino Acids 251 7.3.2 Immobilized Metal
Affinity Chromatography (IMAC) and Mass Spectrometry (MS) 252 7.4 In Vitro
and In Vivo Study of pH-Containing Peptides by Nano-ESI Tandem MS 255 7.4.1
Introduction 255 7.4.2 Background of Study 257 7.4.3 Optimized Methodology
for Phosphohistidine Studies 259 7.4.4 C18 RP LC Behavior 268 7.4.5
Phosphohistidine Loses HPO3 and H3PO4 270 7.4.6 Q-TOF/MS/MS Product Ion
Spectra 277 7.4.7 Behavior of Monophosphohistidine and Diphosphohistidine
Peptide 281 7.4.8 Behavior of Phosphotyrosine and Phosphohistidine Peptide
285 7.4.9 Behavior of Phosphotyrosine-, Phosphothreonine-, and
Phosphohistidine-Containing Peptide 287 7.4.10 Validation of Cu(II)-Based
IMAC Phosphohistidine Enrichment 291 7.4.11 In Vivo Measurement of
Phosphohistidine 293 7.4.12 Gene Ontology of Phosphorylated Proteins 296
7.4.13 Predicted Regulatory Protein Motif Study 307 7.4.14 Validation of
Phosphohistidine-Containing Proteins 308 7.4.15 The pDpH Motif 310 7.4.16
Conclusions 311 7.5 Supplementary Material 311 7.5.1 Reviewing Spectra
Using the SpectrumLook Software Package 311 Reference 313 Appendix I Atomic
Weights and Isotopic Compositions 317 Appendix II Periodic Table of the
Elements 325 Appendix III Fundamental Physical Constants 327 Glossary 329
Index 345