The aim of Molecular Cardiology: Methods and Protocols is to document state-of-the-art molecular and genetic techniques in the area of cardiology. These modern approaches enable researchers to readily study heart diseases at the molecular level and will promote the development of new therapeutic str- egies. Methods for genetic dissection, signal transduction, and microarray analysis are excellent tools for the study of the molecular mechanisms of cardiovascular diseases. Protocols for transgenesis take advantage of recent advances in many areas of molecular and cell biology. Transgenic models…mehr
The aim of Molecular Cardiology: Methods and Protocols is to document state-of-the-art molecular and genetic techniques in the area of cardiology. These modern approaches enable researchers to readily study heart diseases at the molecular level and will promote the development of new therapeutic str- egies. Methods for genetic dissection, signal transduction, and microarray analysis are excellent tools for the study of the molecular mechanisms of cardiovascular diseases. Protocols for transgenesis take advantage of recent advances in many areas of molecular and cell biology. Transgenic models of heart diseases (cardiac hypertrophy, cardiac dysfunction, and so on. ) are powerful tools for the study of heart disease pathogenesis. Methods for gene transfer to heart tissue using viral and nonviral vectors form the basis of gene therapy for heart diseases. Heart-specific promoters containing a hypox- inducible cardioprotective gene switch are key for protection of the heart from ischemia. Gene and stem cell therapies open novel and exciting avenues for the prevention and treatment of heart diseases. Molecular Cardiology: Methods and Protocols consists of 26 chapters de- ing with various aspects of molecular cardiology, including gene transfer and gene therapy for cardiovascular disease, stem cell therapy for cardiovascular disease, gene analysis in the injured and hypertrophied heart, and transgenesis in cardiovascular research. This book provides step-by-step methods for the successful completion of experimental procedures, and would be useful for both experienced and new investigators in the field of molecular cardiology.
Molecular Cardiology is the first major collection of techniques for gene therapy and stem cell therapy for heart diseases. The methods include readily reproducible techniques for genetic dissection, signal transduction, and microarray analysis. Many of the protocols employ not only molecular and cell biology techniques, but also in vivo approaches to myocardial repair using different types of stem cells, and include methods for expansion, purification, and genetic modification of stem cells.
Inhaltsangabe
to Methods in Molecular Cardiology.- Gene Transfer and Gene Therapy for Cardiovascular Disease.- Gene Therapy Approaches to Cardiovascular Disease.- Gene Therapy for Myocardial Ischemia Using the Hypoxia-Inducible Double Plasmid System.- Cardiac Gene Delivery Using DNA-Adenoviral Constructs.- Manipulation of Neuronal Nitric Oxide Synthase Within the Paraventricular Nucleus Using Adenovirus and Antisense Technology.- TAT-Mediated Protein Transduction.- Expression of Human eNOS in Cardiac and Endothelial Cells.- Strategies of Conditional Gene Expression in Myocardium.- Gene Silencing Using Adenoviral RNAi Vector in Vascular Smooth Muscule Cells and Cardiomyocytes.- Stem Cell Therapy for Cardiovascular Disease.- Cardiac Commitment of Embryonic Stem Cells for Myocardial Repair.- Autologous Mesenchymal Stem Cells for Post-Ischemic Myocardial Repair.- Cellular Therapy With Autologous Skeletal Myoblasts for Ischemic Heart Disease and Heart Failure.- Protocols for Myocardial Infarction Repair Using Fetal Cardiac Myocytes.- Methods for Examining Stem Cells in Post-Ischemic and Transplanted Hearts.- Endothelial Progenitor Cell Culture and Gene Transfer.- Gene Analysis in the Injured and Hypertrophied Heart.- Delineation of Sequences Essential for Specific Promoter Activation During Pressure Overloaded Hypertrophy or Factor-Induced Hypertrophy.- Evaluation of the Cardiac Isoform of ?2-Macroglobulin as a Factor Inducing Cardiac Hypertrophy.- Detection of Apoptosis in Cardiovascular Diseases.- Detection of Cardiac Signaling in the Injured and Hypertrophied Heart.- Affymetrix Oligonucleotide Analysis of Gene Expression in the Injured Heart.- Identification and Validation of Loci and Candidate Genes Linked to Cardiac Hypertrophy.- Induction and Analysis of Cardiac Hypertrophy inTransgenic Animal Models.- Methods for the Detection of Altered ?-Adrenergic Receptor Signaling Pathways in Hypertrophied Hearts.- Transgenes in Cardiovascular Research.- Transgenic Mouse Models for Cardiac Dysfunction by a Specific Gene Manipulation.- Cardiomyocyte Defects in Diabetic Models and Protection With Cardiac-Targeted Transgenes.- Cardiac-Specific Overexpression of Angiotensin II Type 1 Receptor in Transgenic Rats.
to Methods in Molecular Cardiology.- Gene Transfer and Gene Therapy for Cardiovascular Disease.- Gene Therapy Approaches to Cardiovascular Disease.- Gene Therapy for Myocardial Ischemia Using the Hypoxia-Inducible Double Plasmid System.- Cardiac Gene Delivery Using DNA-Adenoviral Constructs.- Manipulation of Neuronal Nitric Oxide Synthase Within the Paraventricular Nucleus Using Adenovirus and Antisense Technology.- TAT-Mediated Protein Transduction.- Expression of Human eNOS in Cardiac and Endothelial Cells.- Strategies of Conditional Gene Expression in Myocardium.- Gene Silencing Using Adenoviral RNAi Vector in Vascular Smooth Muscule Cells and Cardiomyocytes.- Stem Cell Therapy for Cardiovascular Disease.- Cardiac Commitment of Embryonic Stem Cells for Myocardial Repair.- Autologous Mesenchymal Stem Cells for Post-Ischemic Myocardial Repair.- Cellular Therapy With Autologous Skeletal Myoblasts for Ischemic Heart Disease and Heart Failure.- Protocols for Myocardial Infarction Repair Using Fetal Cardiac Myocytes.- Methods for Examining Stem Cells in Post-Ischemic and Transplanted Hearts.- Endothelial Progenitor Cell Culture and Gene Transfer.- Gene Analysis in the Injured and Hypertrophied Heart.- Delineation of Sequences Essential for Specific Promoter Activation During Pressure Overloaded Hypertrophy or Factor-Induced Hypertrophy.- Evaluation of the Cardiac Isoform of ?2-Macroglobulin as a Factor Inducing Cardiac Hypertrophy.- Detection of Apoptosis in Cardiovascular Diseases.- Detection of Cardiac Signaling in the Injured and Hypertrophied Heart.- Affymetrix Oligonucleotide Analysis of Gene Expression in the Injured Heart.- Identification and Validation of Loci and Candidate Genes Linked to Cardiac Hypertrophy.- Induction and Analysis of Cardiac Hypertrophy inTransgenic Animal Models.- Methods for the Detection of Altered ?-Adrenergic Receptor Signaling Pathways in Hypertrophied Hearts.- Transgenes in Cardiovascular Research.- Transgenic Mouse Models for Cardiac Dysfunction by a Specific Gene Manipulation.- Cardiomyocyte Defects in Diabetic Models and Protection With Cardiac-Targeted Transgenes.- Cardiac-Specific Overexpression of Angiotensin II Type 1 Receptor in Transgenic Rats.
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