Neural Stem Cell Assays (eBook, ePUB)
Redaktion: Kaur, Navjot; Vemuri, Mohan C.
Neural Stem Cell Assays (eBook, ePUB)
Redaktion: Kaur, Navjot; Vemuri, Mohan C.
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Neural stem cells offer a valuable model system for delineating the cellular and developmental processes in normal and diseased states of the central nervous system. In particular, neural stem cells have huge potential in regenerative medicine, owing to their expansion capability in culture and the ability to differentiate into multiple sub-neural lineages. Neural Stem Cell Assays provides a detailed and comprehensive review of the basic methods for neural stem cell cultures. Including an overview of progress in the field over the past decade, Neural Stem Cell Assays is a one-stop reference…mehr
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- Produktdetails
- Verlag: John Wiley & Sons
- Seitenzahl: 288
- Erscheinungstermin: 24. November 2014
- Englisch
- ISBN-13: 9781118308264
- Artikelnr.: 41868945
- Verlag: John Wiley & Sons
- Seitenzahl: 288
- Erscheinungstermin: 24. November 2014
- Englisch
- ISBN-13: 9781118308264
- Artikelnr.: 41868945
Cells 1 Mohan C. Vemuri 1.1 Introduction 1 1.2 NSC Derivation from Rosette
Formation of Embryoid Bodies 1 1.3 Rosette Free NSC in a Monolayer Culture
2 1.4 Pathways Involved in Neural Tube and Neural Crest Lineages 2 1.5
Differentiation and Gene Expression in Human Brain Development 3 1.6
Conclusions 6 References 6 2 The History and Design of Assays for the
Identification and Characterization of Neural Stem Cells 9 Sharon A. Louis
and Brent A. Reynolds 2.1 Introduction 9 2.2 Materials 12 2.3 Methods 14
2.4 Notes 21 Acknowledgments 23 References 23 3 Culture of Human Neural
Stem Cells 25 Soojung Shin, Yiping Yan and Mohan C Vemuri 3.1 Introduction
25 3.2 Materials 25 3.3 Methods 30 3.4 Notes 33 3.5 Expected Results 33
References 34 4 GMP4-Compatible Production and Expansion of Human Neural
Stem Cells 35 David J. Brick, Alexander E. Stover, Hubert E. Nethercott,
Maria G. Banuelos and Philip H. Schwartz 4.1 Introduction 35 4.2 Materials
36 4.3 Methods 40 4.4 Notes 50 Acknowledgments 52 References 52 5 Primary
Rat Neural Cultures 55 Navjot Kaur 5.1 Introduction 55 5.2 Materials 55 5.3
Methods 57 5.4 Notes 59 References 59 6 Cryopreservation of Human Neural
Stem and Progenitor Cells 61 Profico Daniela Celeste, Sgaravizzi Giada,
Projetti Pensi Massimo, Muzi Gianmarco, Ricciolini Claudia, Gelati Maurizio
and Vescovi Angelo Luigi 6.1 Introduction 61 6.2 Materials 62 6.3 Methods
62 6.4 Notes 65 References 65 7 Cryopreservation and Recovery of Primary
Rat Neural Cells 67 Rhonda Newman and Navjot Kaur 7.1 Introduction 67 7.2
Materials 67 7.3 Methods 68 7.4 Notes 70 References 70 8 Efficient
Induction and Scale up Generation of Neural Stem Cells from Human
Pluripotent Stem Cells 71 Yiping Yan, Soojung Shin and Mohan C. Vemuri 8.1
Introduction 71 8.2 Materials 71 8.3 Methods 73 8.4 Notes 78 References 79
9 Directed Differentiation of Human NSC/NPC into Dopaminergic Neurons 81
Andrzej Swistowski and Xianmin Zeng 9.1 Introduction 81 9.2 Materials 82
9.3 Methods 83 9.4 Notes 88 References 88 10 In vitro Differentiation of
Pluripotent Stem Cells towards either Forebrain GABAergic or Midbrain
Dopaminergic Neurons 91 Brigham J. Hartley, Bradley Watmuff, Cameron P.J.
Hunt, John M. Haynes and Colin W. Pouton 10.1 Introduction 91 10.2
Materials 91 10.3 Methods 94 10.4 Notes 97 10.5 Typical Results 97
References 98 11 Generation of Astrocytes from Human Pluripotent Stem Cells
using a Defined System 101 Atossa Shaltouki and Xianmin Zeng 11.1
Introduction 101 11.2 Materials and Methods 101 11.3 Notes 108 References
108 12 Directed Differentiation of Human PSC into Oligodendrocytes 111 Anu
Hyysalo and Susanna Narkilahti 12.1 Introduction 111 12.2 Materials 111
12.3 Methods 113 12.4 Notes 116 Acknowledgments 117 References 117 13
Directed Differentiation towards Human Neural Retinal Cells 119 Deepak A.
Lamba 13.1 Introduction 119 13.2 Materials 120 13.3 Methods 121 References
126 14 Directed Differentiation of Photoreceptors and Retinal Pigment
Epithelium from Adult Mouse Retinal Stem Cells 129 Brian G. Ballios,
Valeria Marigo and Derek van der Kooy 14.1 Introduction 129 14.2 Materials
130 14.3 Methods 131 14.4 Notes 134 Acknowledgments 134 References 134 15
Induction of Schwann Cells from Rat Bone Marrow Mesenchymal Stem Cells 137
Shohei Wakao, Masaaki Kitada and Mari Dezawa 15.1 Introduction 137 15.2
Materials 138 15.3 Method 141 15.4 Notes 149 References 151 16 Neural Cell
Viability Assays 155 Bonnie J. Hanson and Michael K. Hancock 16.1
Introduction 155 16.2 Materials 157 16.3 Methods 158 16.4 Notes 165
References 165 17 Genetic Modification of Pluripotent Stem Cell Derived
Human Neural Progenitor Cells 167 Jamie M. Chilton and Steven L. Stice 17.1
Introduction 167 17.2 Materials 168 17.3 Methods 170 17.4 Notes 176
Acknowledgments 178 References 178 18 Electrophysiology of Neurons 179
Christine M. Emnett and Steven Mennerick 18.1 Introduction 179 18.2
Materials 180 18.3 Methods 181 18.4 Notes 185 References 187 19 Induction
and Transplantation of Neural Stem Cells from Human Pluripotent Stem Cells
in Experimental Models (Rat) of Parkinson's disease 189 Makoto Motono,
Asuka Morizane and Jun Takahashi 19.1 Introduction 189 19.2 Materials 189
19.3 Methods 191 19.4 Notes 194 Acknowledgments 194 References 194 20
Transplantation of Human Neural Stem Cells and Progenitors in Animal Models
of Disease 197 Joseph F. Bonner and Itzhak Fischer 20.1 Introduction 197
20.2 General Principles of Effective Transplantation 198 20.3 Different
Methods of Transplantation 204 20.4 Materials and Method 205 20.5
Conclusions 211 20.6 Notes 212 Acknowledgments 212 References 212 21
Morphometric Assays for Neural Differentiation and Synaptic Development of
Human iPSC-Derived NSC 215 Barbara Calabrese, Leif Dehmelt and Shelley
Halpain 21.1 Introduction 215 21.2 Materials 216 21.3 Method 218 21.4 Notes
222 Acknowledgments 223 References 223 22 BacMam Transduction of Neural
Stem Cells 225 Rene Quintanilla and Uma Lakshmipathy 22.1 Introduction 225
22.2 Materials 225 22.3 Methods 227 22.4 Notes 228 Acknowledgments 229
References 229 23 The Proximity Ligation Assay: A High Throughput Technique
for Protein Analysis in Neuroscience 231 Michela Zaltieri, PierFranco
Spano, Cristina Missale and Arianna Bellucci 23.1 Introduction 231 23.2
Materials 232 23.3 Methods 232 23.4 Notes 238 23.5 Troubleshooting 238
Acknowledgments 240 References 240 24 Quantification of Micro-RNA
Expression in Human Neural Stem Cells using TaqMan Assays 241 Xavier Nissan
and Alexandra Benchoua 24.1 Introduction 241 24.2 Materials 242 24.3
Methods 244 24.4 Notes 248 References 249 25 Genetic Reporter Cell Lines:
Tools for Stem Cell Biology and Drug Discovery 251 Cameron P.J. Hunt,
Bradley Watmuff, Brigham J. Hartley, Colin W. Pouton and John M. Haynes
25.1 Pluripotent Stem Cell Reporter Lines 251 25.2 Choice of PSC Genetic
Reporter Systems 251 25.3 Reporter Systems 252 25.4 Multiple Reporter Lines
254 25.5 Use of Genetic Reporter Escs to Study Neural Differentiation 255
References 260 Index 261
Cells 1 Mohan C. Vemuri 1.1 Introduction 1 1.2 NSC Derivation from Rosette
Formation of Embryoid Bodies 1 1.3 Rosette Free NSC in a Monolayer Culture
2 1.4 Pathways Involved in Neural Tube and Neural Crest Lineages 2 1.5
Differentiation and Gene Expression in Human Brain Development 3 1.6
Conclusions 6 References 6 2 The History and Design of Assays for the
Identification and Characterization of Neural Stem Cells 9 Sharon A. Louis
and Brent A. Reynolds 2.1 Introduction 9 2.2 Materials 12 2.3 Methods 14
2.4 Notes 21 Acknowledgments 23 References 23 3 Culture of Human Neural
Stem Cells 25 Soojung Shin, Yiping Yan and Mohan C Vemuri 3.1 Introduction
25 3.2 Materials 25 3.3 Methods 30 3.4 Notes 33 3.5 Expected Results 33
References 34 4 GMP4-Compatible Production and Expansion of Human Neural
Stem Cells 35 David J. Brick, Alexander E. Stover, Hubert E. Nethercott,
Maria G. Banuelos and Philip H. Schwartz 4.1 Introduction 35 4.2 Materials
36 4.3 Methods 40 4.4 Notes 50 Acknowledgments 52 References 52 5 Primary
Rat Neural Cultures 55 Navjot Kaur 5.1 Introduction 55 5.2 Materials 55 5.3
Methods 57 5.4 Notes 59 References 59 6 Cryopreservation of Human Neural
Stem and Progenitor Cells 61 Profico Daniela Celeste, Sgaravizzi Giada,
Projetti Pensi Massimo, Muzi Gianmarco, Ricciolini Claudia, Gelati Maurizio
and Vescovi Angelo Luigi 6.1 Introduction 61 6.2 Materials 62 6.3 Methods
62 6.4 Notes 65 References 65 7 Cryopreservation and Recovery of Primary
Rat Neural Cells 67 Rhonda Newman and Navjot Kaur 7.1 Introduction 67 7.2
Materials 67 7.3 Methods 68 7.4 Notes 70 References 70 8 Efficient
Induction and Scale up Generation of Neural Stem Cells from Human
Pluripotent Stem Cells 71 Yiping Yan, Soojung Shin and Mohan C. Vemuri 8.1
Introduction 71 8.2 Materials 71 8.3 Methods 73 8.4 Notes 78 References 79
9 Directed Differentiation of Human NSC/NPC into Dopaminergic Neurons 81
Andrzej Swistowski and Xianmin Zeng 9.1 Introduction 81 9.2 Materials 82
9.3 Methods 83 9.4 Notes 88 References 88 10 In vitro Differentiation of
Pluripotent Stem Cells towards either Forebrain GABAergic or Midbrain
Dopaminergic Neurons 91 Brigham J. Hartley, Bradley Watmuff, Cameron P.J.
Hunt, John M. Haynes and Colin W. Pouton 10.1 Introduction 91 10.2
Materials 91 10.3 Methods 94 10.4 Notes 97 10.5 Typical Results 97
References 98 11 Generation of Astrocytes from Human Pluripotent Stem Cells
using a Defined System 101 Atossa Shaltouki and Xianmin Zeng 11.1
Introduction 101 11.2 Materials and Methods 101 11.3 Notes 108 References
108 12 Directed Differentiation of Human PSC into Oligodendrocytes 111 Anu
Hyysalo and Susanna Narkilahti 12.1 Introduction 111 12.2 Materials 111
12.3 Methods 113 12.4 Notes 116 Acknowledgments 117 References 117 13
Directed Differentiation towards Human Neural Retinal Cells 119 Deepak A.
Lamba 13.1 Introduction 119 13.2 Materials 120 13.3 Methods 121 References
126 14 Directed Differentiation of Photoreceptors and Retinal Pigment
Epithelium from Adult Mouse Retinal Stem Cells 129 Brian G. Ballios,
Valeria Marigo and Derek van der Kooy 14.1 Introduction 129 14.2 Materials
130 14.3 Methods 131 14.4 Notes 134 Acknowledgments 134 References 134 15
Induction of Schwann Cells from Rat Bone Marrow Mesenchymal Stem Cells 137
Shohei Wakao, Masaaki Kitada and Mari Dezawa 15.1 Introduction 137 15.2
Materials 138 15.3 Method 141 15.4 Notes 149 References 151 16 Neural Cell
Viability Assays 155 Bonnie J. Hanson and Michael K. Hancock 16.1
Introduction 155 16.2 Materials 157 16.3 Methods 158 16.4 Notes 165
References 165 17 Genetic Modification of Pluripotent Stem Cell Derived
Human Neural Progenitor Cells 167 Jamie M. Chilton and Steven L. Stice 17.1
Introduction 167 17.2 Materials 168 17.3 Methods 170 17.4 Notes 176
Acknowledgments 178 References 178 18 Electrophysiology of Neurons 179
Christine M. Emnett and Steven Mennerick 18.1 Introduction 179 18.2
Materials 180 18.3 Methods 181 18.4 Notes 185 References 187 19 Induction
and Transplantation of Neural Stem Cells from Human Pluripotent Stem Cells
in Experimental Models (Rat) of Parkinson's disease 189 Makoto Motono,
Asuka Morizane and Jun Takahashi 19.1 Introduction 189 19.2 Materials 189
19.3 Methods 191 19.4 Notes 194 Acknowledgments 194 References 194 20
Transplantation of Human Neural Stem Cells and Progenitors in Animal Models
of Disease 197 Joseph F. Bonner and Itzhak Fischer 20.1 Introduction 197
20.2 General Principles of Effective Transplantation 198 20.3 Different
Methods of Transplantation 204 20.4 Materials and Method 205 20.5
Conclusions 211 20.6 Notes 212 Acknowledgments 212 References 212 21
Morphometric Assays for Neural Differentiation and Synaptic Development of
Human iPSC-Derived NSC 215 Barbara Calabrese, Leif Dehmelt and Shelley
Halpain 21.1 Introduction 215 21.2 Materials 216 21.3 Method 218 21.4 Notes
222 Acknowledgments 223 References 223 22 BacMam Transduction of Neural
Stem Cells 225 Rene Quintanilla and Uma Lakshmipathy 22.1 Introduction 225
22.2 Materials 225 22.3 Methods 227 22.4 Notes 228 Acknowledgments 229
References 229 23 The Proximity Ligation Assay: A High Throughput Technique
for Protein Analysis in Neuroscience 231 Michela Zaltieri, PierFranco
Spano, Cristina Missale and Arianna Bellucci 23.1 Introduction 231 23.2
Materials 232 23.3 Methods 232 23.4 Notes 238 23.5 Troubleshooting 238
Acknowledgments 240 References 240 24 Quantification of Micro-RNA
Expression in Human Neural Stem Cells using TaqMan Assays 241 Xavier Nissan
and Alexandra Benchoua 24.1 Introduction 241 24.2 Materials 242 24.3
Methods 244 24.4 Notes 248 References 249 25 Genetic Reporter Cell Lines:
Tools for Stem Cell Biology and Drug Discovery 251 Cameron P.J. Hunt,
Bradley Watmuff, Brigham J. Hartley, Colin W. Pouton and John M. Haynes
25.1 Pluripotent Stem Cell Reporter Lines 251 25.2 Choice of PSC Genetic
Reporter Systems 251 25.3 Reporter Systems 252 25.4 Multiple Reporter Lines
254 25.5 Use of Genetic Reporter Escs to Study Neural Differentiation 255
References 260 Index 261