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Produktbeschreibung
  • Produktdetails
  • Verlag: Springer Berlin
  • Erscheinungstermin: 23. Dezember 2009
  • Englisch
  • ISBN-13: 9783642049286
  • Artikelnr.: 37368383
Inhaltsangabe

General Remarks.- Detection, Profiling, and Quantification of miRNA Expression.- miRNA Microarray Methods.- Microarray and Its Variants for miRNA Profiling.- Northern Blotting Methods.- Northern Blotting and Its Variants for Detecting Expression and Analyzing Tissue Distribution of miRNAs.- In Situ Hybridization Methods.- In Situ Hybridization and Its Variants for Detecting Expression and Analyzing Cellular Distribution of miRNAs.- Real-Time RT-PCR Methods.- End-Point Stem-Loop Real-Time RT-PCR for miRNA Quantification.- miR-Q RT-PCR for miRNA Quantification.- Poly(A)-Tailed Universal Reverse Transcription.- Multiplexing RT-PCR for High-Throughput miRNA Profiling.- miRNA Amplification Profiling (mRAP).- Cloning Methods.- miRNA Serial Analysis of Gene Expression (miRAGE or SAGE).- Nanoparticle Methods.- Electrocatalytic Nanoparticle Tags Technique for High-Sensitivity miRNA Expression Analysis.- Nanoparticle-Amplified SPR Imaging for High-Sensitivity miRNA Profiling.- Conducting Polymer Nanowires Technique for High-Sensitivity miRNA Expression Analysis.- Gold Nanoparticle Probe Method for miRNA Quantification.- Other Methods.- Splinted Ligation Method.- Padlock-Probes and Rolling-Circle Amplification.- Invader Assay.- Single Molecule Method.- Enzymatic Method.- Surface-Enhanced Raman Spectroscopy Method.- RAKE Assay.- Bead-Based Flow Cytometric miRNA Expression Profiling.- Bioluminescence miRNA Detection Method.- Molecular Beacon Method.- Ribozyme Method.- Electrocatalytic Moiety Labeling Technique for High-Sensitivity miRNA Expression Analysis.- Circulating miRNA Detection Methods.- Serum and Plasma miRNA Detection.- miRNA Detection from Peripheral Blood Microvesicles.- Detection of Placental miRNAs in Maternal Plasma.- Single-cell miRNA Detection Methods.- Quantitative LNA-ELF-FISH Method for miRNA Detection in Single Mammalian Cell.- Single Cell Stem-Looped Real-Time PCR.- miRNA Function-Reporter Expression Assay.- Whole Mount In Situ Analysis.- Whole Mount In Situ Hybridization (WM-ISH) for miRNA Expression Profiling During Vertebrate Development.

General Remarks.- Detection, Profiling, and Quantification of miRNA Expression.- miRNA Microarray Methods.- Microarray and Its Variants for miRNA Profiling.- Northern Blotting Methods.- Northern Blotting and Its Variants for Detecting Expression and Analyzing Tissue Distribution of miRNAs.- In Situ Hybridization Methods.- In Situ Hybridization and Its Variants for Detecting Expression and Analyzing Cellular Distribution of miRNAs.- Real-Time RT-PCR Methods.- End-Point Stem-Loop Real-Time RT-PCR for miRNA Quantification.- miR-Q RT-PCR for miRNA Quantification.- Poly(A)-Tailed Universal Reverse Transcription.- Multiplexing RT-PCR for High-Throughput miRNA Profiling.- miRNA Amplification Profiling (mRAP).- Cloning Methods.- miRNA Serial Analysis of Gene Expression (miRAGE or SAGE).- Nanoparticle Methods.- Electrocatalytic Nanoparticle Tags Technique for High-Sensitivity miRNA Expression Analysis.- Nanoparticle-Amplified SPR Imaging for High-Sensitivity miRNA Profiling.- Conducting Polymer Nanowires Technique for High-Sensitivity miRNA Expression Analysis.- Gold Nanoparticle Probe Method for miRNA Quantification.- Other Methods.- Splinted Ligation Method.- Padlock-Probes and Rolling-Circle Amplification.- Invader Assay.- Single Molecule Method.- Enzymatic Method.- Surface-Enhanced Raman Spectroscopy Method.- RAKE Assay.- Bead-Based Flow Cytometric miRNA Expression Profiling.- Bioluminescence miRNA Detection Method.- Molecular Beacon Method.- Ribozyme Method.- Electrocatalytic Moiety Labeling Technique for High-Sensitivity miRNA Expression Analysis.- Circulating miRNA Detection Methods.- Serum and Plasma miRNA Detection.- miRNA Detection from Peripheral Blood Microvesicles.- Detection of Placental miRNAs in Maternal Plasma.- Single-cell miRNA Detection Methods.- Quantitative LNA-ELF-FISH Method for miRNA Detection in Single Mammalian Cell.- Single Cell Stem-Looped Real-Time PCR.- miRNA Function-Reporter Expression Assay.- Whole Mount In Situ Analysis.- Whole Mount In Situ Hybridization (WM-ISH) for miRNA Expression Profiling During Vertebrate Development.
Rezensionen
From the reviews:
"Designed primarily as a reference for life-science researchers, this book enables investigators to make unbiased comparisons in the search for the best technique for their unique application. This book is organized into 11 major parts ... . MicroRNA Expression Detection Methods is a concise, logical presentation of the most relevant microRNA detection methods currently in use. As a procedure reference, this work contributes significantly to the field of microRNA research and will serve as a valuable laboratory resource for life-science professionals." (David Broyles and Sapna K. Deo, Analytical and Bioanalytical Chemistry, Vol. 399, 2011)