Charles L. Wilkins, Jackson O. Lay

Identification of Microorganisms by Mass Spectrometry (eBook, PDF)

• Format: PDF

Produktbeschreibung

A multidisciplinary approach to understanding the fundamentals of mass spectrometry for bacterial analysis From chemotaxonomy to characterization of targeted proteins, Identification of Microorganisms by Mass Spectrometry provides an overview of both well-established and cutting-edge mass spectrometry techniques for identifying microorganisms. A vital tool for microbiologists, health professionals, and analytical chemists, the text is designed to help scientists select the most effective techniques for use in biomedical, biochemical, pharmaceutical, and bioterror defense applications. Since microbiological applications of mass spectrometry require a basic understanding of both microbiology and analytical chemistry, the editors have incorporated material from both disciplines so that readers from either field will come to understand the necessary principles of the other. Featuring contributions from some of the most recognized experts in both fields, this volume provides specific examples of fundamental methods as well as approaches developed in the last decade, including: * Metastable atom bombardment pyrolysis mass spectrometry * Matrix-assisted laser desorption/ionization mass spectrometry (MALDI) * MALDI time-of-flight mass spectrometry (MALDI-TOF MS) of intact bacteria * High-resolution Fourier transform mass spectrometry (FTMS) * Electrospray ionization (ESI) mass spectrometry Identification of Microorganisms by Mass Spectrometry represents the most comprehensive and up-to-date work on the topic currently available. It is liberally illustrated with figures and tables and covers every aspect of spectrometric identification of microorganisms, including experimental procedures, various means of sample preparation, data analysis, and interpretation of complex mass spectral data.

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Produktdetails

• Verlag: John Wiley & Sons
• Seitenzahl: 352
• Erscheinungstermin: 13. Dezember 2005
• Englisch
• ISBN-13: 9780471748632
• Artikelnr.: 37363672

Autorenporträt

CHARLES L. WILKINS, PhD, is a Distinguished Professor in the Department of Chemistry and Biochemistry at the University of Arkansas. He is the author or coauthor of over 250 scientific publications and has received numerous awards for his research. JACKSON O. LAY, Jr., PhD, is the Director of the Arkansas Statewide Mass Spectrometry Facility at the University of Arkansas, and the author or coauthor of more than 100 publications in the field. In addition to his work with bacteria, he has developed mass spectrometry methods for cancer research and toxicology.

Inhaltsangabe

Preface. Contributors. 1. Cultural, Serological and Genetic Methods for
Identification of Bacteria (John B. Sutherland and Fatemeh Rafii). 2. Mass
Spectrometry: Identification and Biodetection, Lessons Learned and Future
Developments (Alvin Fox). 3. An Introduction to Maldi TOF MS Analysis of
Whole Bacteria (Rohana Liyanage and Jackson O. Lay, Jr.). 4. The
Development of the Block II Chemical Biological Mass Spectrometer (Wayne H.
Griest and Stephen A. Lammert. 5. Method Reproducibility and Spectral
Library Assembly for Rapid Bacterial Characterization by Metastable Atom
Bombardment Pyrolysis Mass Spectrometry (Jon G. Wilkes, Gary Miertschin,
Todd Eschler, Les Hosey, Fatemeh Rafii, Larry Rushing, Dan A. Buzatu, and
Michel J. Bertrand). 6. MALDI TOF Mass Spectrometry of Intact Bacteria
(Jackson O. Lay, Jr., and Rohana Liyanage). 7. Development of Spectral
Pattern Matching Approaches to Matrix-Assisted Laser Desorption/Ionization
Time-of-Flight Mass Spectrometry for Bacterial Identification (Kristin H.
Jarman and Karen L. Wahl). 8. Studies of Malaria by Mass Spectrometry
(Plamen A. Demirev). 9. Bacterial Strain Differentiation by Mass
Spectrometry (Randy J. Arnold, Jonathan A. Karty and James P. Reilly). 10.
Bacterial Protein Biomarker Discovery: A Focused Approach to Developing
Molecular Based Identification Systems (Tracie L. Williams, Steven R.
Monday, and Steven M. Musser). 11. High Throughput Microbial
Characterisations Using Electrospray Ionisation Mass Spectrometry and Its
Role in Functional Genomics (Seetharaman Vaidyanathan and Royston
Goodacre). 12. Bioinformatics for Flexibility, Reliability and Mixture
Analysis of Intact Microorganisms (Catherine Fenselau and Patrick Pribil).
13. MALDI-FTMS of Whole Cell Bacteria (Jeffrey J. Jones, Michael J. Stump,
and Charles L. Wilkins). 14. A Review of Antibody Capture and Bacteriophage
Amplification in Connection with the Direct Analysis of Whole Cell Bacteria
by MALDI-TOF-MS (Kent J. Voorhees and Jon C. Rees). 15. Discrimination and
Identification of Microorganisms by Ppyrolysis Mass Spectrometry: From
Burning Ambitions to Cooling Embers - A Historical Perspective (Éadaoin
Timmins and Royston Goodacre). Index.