Tim Wing Yam, Joseph Arditti

Micropropagation of Orchids (eBook, PDF)

• Format: PDF

Produktbeschreibung

Divided into three volumes, Micropropagation of Orchids Third Edition retains the exhaustive list of micropropagation protocols for many genera and updates each section to include new and/or revised information about: * Culture media and vessels * Techniques and procedures for both orchids which were previously cultured and for those which were not * Plant hormones and growth regulators * Media components * Methods for tissue decontamination * Historical information * Procedures for the cultivation for plantlets which have been removed from flasks * Sources of light and illumination methods Written by two globally acknowledged experts in the field, the third edition of this definitive text on the micropropagation of orchids is a detailed and comprehensive collection of procedures and methods for multiplying orchids, including organ, tissue, and cell culture techniques in vitro and is intended for researchers in plant science and propagation, professional and amateur orchid growers, and plant breeding professionals. Much of the general information about techniques and procedures can be applied to plants other than orchids.

Produktdetails

• Verlag: John Wiley & Sons
• Seitenzahl: 2368
• Erscheinungstermin: 5. Juli 2017
• Englisch
• ISBN-13: 9781119187066
• Artikelnr.: 52580497

Autorenporträt

Tim Wing Yam, Singapore Botanic Gardens, National Parks Board, Singapore Joseph Arditti, Professor Emeritus, University of California, Irvine, USA

Inhaltsangabe

Preface Preface to the Second Edition Preface to the First Edition Chapter 1: History Terminology Origins of Orchid Micropropagation Plant Hormones and Propagation Additives of Plant Origin Culture of Tissues and Organs Early Tissue Culture Attempts Culture of Stem Tips First Micropropagation of Orchids The Second Aseptic Culture of an Orchid Explant Plant Diseases and Meristems The Third Aseptic Culture of an Orchid Explant The Fourth Aseptic Culture of an Orchid Explant Who Pioneered Micropropagation? Root Cultures Leaf Cultures Stems Flower Buds, Flowers, Floral Segments, and Reproductive Organs Inflorescences A Patent Doubtful Claims Mutations Theft in Vitro Darkening of Culture Media Anticontaminants Cell and Protoplast Culture Flowering in Vitro, The Future Chapter 2: General Outline of Techniques and Procedures Media Components Macroelements Microelements Iron Auxins and Anti-auxin Chitosan Cytokinins Gibberellins and Antigibberellin Abscisic Acid Ethylene and Ethylene Inhibitor Amino Acids Polyol Polyamines Phloroglucinol Jasmonates Vitamins Nucleotides and Nucleic Acids Organic Acids Banana Coconut Water Hydrolysates and Autolysates Sugars Anticontaminants Charcoal Solvents Solidifiers pH Recipes in this Book Stock Solutions State of the Medium Sterilization Autoclaves Filtration Sterilization in Microwave Ovens Solvents Open Flame Liquids Surface Decontamination Gas Sterilization Preparation of the Medium Culture Vessels Culture Conditions Temperature Agitation Illumination Carbon Dioxide Placing Plant Material in Culture Seeds Tissue Explants Internal Contaminants Enzymes for Protoplast Isolation Work Area Laminar-flow Hoods Sterile Rooms Sterile Boxes Clean Laboratory Bench Washing Glassware Chapter 3: Methods for Specific Genera Acampe Aeridachnis Aerides Aeridovanda Anacamptis Angraecum Anoectochilus Ansellia Arachnis Arachnostylis Aranda Aranthera Arundina Ascocenda Ascocentrum Ascofinetia Barlia Bletilla Brassavola Brassia Brassocattleya Brassolaeliocattleya, 277 Bulbophyllum Burkillara Caladenia Calanthe Catasetum Cattleya Caularthron Cleisostoma Clowesia Coelogyne Cymbidium Cypripedium Cyrtopodium Dactylorchis Dactylorhiza Darwinara Dendrobium, 518 Disa Diuris Doriella Doritaenopsis Doritis, 693 Encyclia Epidendrum Epiphronitis Erycina Eulophia Gastrochilus Geodorum Goodyera Grammatophyllum Habenaria Hadrolaelia Hetaeria Holttumara Ipsea Kagawara Laelia Laeliocattleya Liparis Ludisia (Haemaria) Luisia Lycaste Malaxis Maxillaria Miltonia Mokara Mormodes Neofinetia Neostylis Neottia Nervilia Nigritella Oberonia Odontioda Odontoglossum Odontonia Oncidium Ophrys Orchis Ottochilus Pachystoma Paphiopedilum Papilionanda Papilionanthe, 901 Phaius Phalaenopsis Pholidota Phragmipedium Pleione Pogonia Ponerorchis Potinara Pterostylis Renades Renanetia Renantanda Renanthera Restrepia Rhynchostele Rhynchostylis Saccolabium Sarcanthus Satyrium Schomburgkia Serapias Sophrolaeliocattleya Spathoglottis Spiranthes Tainia Thelymitra Thunia Trudelia Vanda Vandofinetia Vanilla Vascostylis Vuylstekeara, 1292 Zygopetalum, 1292 Chapter 4: Summary Culture Conditions Media Components Macroelements Microelements Substitutions Carbohydrates Amino Acids Vitamins Polyols Hormones Coconut Water Bleeding Sap of Birch Trees Other Complex Organic Additives Solidifying (Gel-forming) Agents and Alternative Supporting Media Adsorbents Applications of Orchid Tissue Culture Commercialization Conservation Patents Plant Improvement and Biotechnology Cryopreservation Physiological Studies Negative Aspects of Micropropagation References Appendix 1: General Information on Supplies, Equipment, Terms, and Reagents Appendix 2: Sources of Supplies and Equipment Appendix 3: Some Sites of Interest on the World Wide Web Appendix 4: Light Appendix 5: Formulary Appendix 6: Atomic Weights, Concentrations, Exponents, Greek and Roman Letters, Ions, Measurements, Molecular Weights, Prefixes, Valences, Units, and Solutions Appendix 7: Additional Information Appendix 8: Plant Preservative Mixture Appendix 9: Media Appendix 10: ASCII Codes Glossary Index

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Preface

Preface to the Second Edition

Preface to the First Edition

Chapter 1: History

Terminology

Origins of Orchid Micropropagation

Plant Hormones and Propagation Additives of Plant Origin

Culture of Tissues and Organs

Early Tissue Culture Attempts

Culture of Stem Tips

First Micropropagation of Orchids

The Second Aseptic Culture of an Orchid Explant

Plant Diseases and Meristems

The Third Aseptic Culture of an Orchid Explant

The Fourth Aseptic Culture of an Orchid Explant

Who Pioneered Micropropagation?

Root Cultures

Leaf Cultures

Stems

Flower Buds, Flowers, Floral Segments, and Reproductive Organs

Inflorescences

A Patent

Doubtful Claims

Mutations

Theft in Vitro

Darkening of Culture Media

Anticontaminants

Cell and Protoplast Culture

Flowering in Vitro,

The Future

Chapter 2: General Outline of Techniques and Procedures

Media Components

Macroelements

Microelements

Iron

Auxins and Anti-auxin

Chitosan

Cytokinins

Gibberellins and Antigibberellin

Abscisic Acid

Ethylene and Ethylene Inhibitor

Amino Acids

Polyol

Polyamines

Phloroglucinol

Jasmonates

Vitamins

Nucleotides and Nucleic Acids

Organic Acids

Banana

Coconut Water

Hydrolysates and Autolysates

Sugars

Anticontaminants

Charcoal

Solvents

Solidifiers

pH

Recipes in this Book

Stock Solutions

State of the Medium

Sterilization

Autoclaves

Filtration

Sterilization in Microwave Ovens

Solvents

Open Flame

Liquids

Surface Decontamination

Gas Sterilization

Preparation of the Medium

Culture Vessels

Culture Conditions

Temperature

Agitation

Illumination

Carbon Dioxide

Placing Plant Material in Culture

Seeds

Tissue Explants

Internal Contaminants

Enzymes for Protoplast Isolation

Work Area

Laminar-flow Hoods

Sterile Rooms

Sterile Boxes

Clean Laboratory Bench

Washing Glassware

Chapter 3: Methods for Specific Genera

Acampe

Aeridachnis

Aerides

Aeridovanda

Anacamptis

Angraecum

Anoectochilus

Ansellia

Arachnis

Arachnostylis

Aranda

Aranthera

Arundina

Ascocenda

Ascocentrum

Ascofinetia

Barlia

Bletilla

Brassavola

Brassia

Brassocattleya

Brassolaeliocattleya, 277

Bulbophyllum

Burkillara

Caladenia

Calanthe

Catasetum

Cattleya

Caularthron

Cleisostoma

Clowesia

Coelogyne

Cymbidium

Cypripedium

Cyrtopodium

Dactylorchis

Dactylorhiza

Darwinara

Dendrobium, 518

Disa

Diuris

Doriella

Doritaenopsis

Doritis, 693

Encyclia