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Protein Chromatography Methods and Protocols

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Beschreibung

This third edition expands on the previous editions with updated and new chapters on protein chromatography. Chapters detail protein stability and storage, avoiding proteolysis, protein quantitation methods, generation and purification of recombinant proteins, recombinant antibody production, and the...

Beschreibung

This third edition expands on the previous editions with updated and new chapters on protein chromatography. Chapters detail protein stability and storage, avoiding proteolysis, protein quantitation methods, generation and purification of recombinant proteins, recombinant antibody production, and the tagging of proteins. Written in the format of the highly successful 
Methods in Molecular Biology 
series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols.

 


Authoritative and cutting-edge, 
Protein Chromatography: Methods and Protocols, Third Edition
aims to provide commonly used methods and new approaches to help both new researchers and experts expand their knowledge. 

Portrait

.-

Produktdetails

Einband

Gebundene Ausgabe

Erscheinungsdatum

31.08.2023

Abbildungen

X, 498 p. 47 illus., 39 illus. in color.

Herausgeber Sinéad T. Loughran + weitere

Produktdetails

Einband

Gebundene Ausgabe

Erscheinungsdatum

31.08.2023

Abbildungen

X, 498 p. 47 illus., 39 illus. in color.

Herausgeber
Verlag Springer Us
Seitenzahl

498

Maße (L/B/H)

26/18,3/3,3 cm

Gewicht

1079 g

Auflage

Third Edition 2023

Sprache

Englisch

ISBN

978-1-07-163361-8

Herstelleradresse

Libri GmbH
Europaallee 1
36244 Bad Hersfeld
DE

Email: gpsr@libri.de

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  • Produktbild: Protein Chromatography
  • Produktbild: Protein Chromatography

  • A Synopsis of Proteins and Their Purification.- Mixed-mode chromatography and its role in monoclonal antibody purification.- Application of Continuous Counter-current Chromatography in Protein Purification.- Chromatographic Purification of Viral Vectors for Gene Therapy Applications.- Scale-up of Protein Purification -Downstream Processing Issues.- Approaches to avoid Proteolysis During Protein Expression and Purification.- Tagging Recombinant Proteins to Enhance Solubility and Aid Purification.- Magnetic nanoparticles for protein separation and purification.- Ion-Exchange Chromatography: Basic Principles and Application.- Hydroxyapatite Chromatography.- Poly-Histidine-Tagged Protein Purification using IMAC.- Lectin Affinity Chromatography.- Single-step non-chromatographic purification of recombinant mammalian proteins using a split intein ELP tag system.- Clinical Proteomics: Liquid Chromatography-Mass Spectrometry (LC/MS) Purification Systems.- Immunoprecipitation: Variations, Considerations, and Applications.- Protein Quantitation and Analysis of Purity.- Protein Extraction and Purification by Differential Solubilisation.- Protein Stability: Enhancement and Measurement.- Storage and Lyophilisation of Pure Proteins.- Strategies for the Purification of Membrane Proteins.