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FOXO Transcription Factors Methods and Protocols

Aus der Reihe Methods in Molecular Biology
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Beschreibung

Produktdetails

Einband

Taschenbuch

Erscheinungsdatum

10.12.2019

Herausgeber

Wolfgang Link

Verlag

Springer Us

Seitenzahl

270

Maße (L/B/H)

25,4/17,8/1,6 cm

Gewicht

552 g

Auflage

Softcover reprint of the original 1st ed. 2019

Sprache

Englisch

ISBN

978-1-4939-9409-0

Beschreibung

Produktdetails

Einband

Taschenbuch

Erscheinungsdatum

10.12.2019

Herausgeber

Wolfgang Link

Verlag

Springer Us

Seitenzahl

270

Maße (L/B/H)

25,4/17,8/1,6 cm

Gewicht

552 g

Auflage

Softcover reprint of the original 1st ed. 2019

Sprache

Englisch

ISBN

978-1-4939-9409-0

Herstelleradresse

Libri GmbH
Europaallee 1
36244 Bad Hersfeld
DE

Email: GPSR Kontakt

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  • Produktbild: FOXO Transcription Factors

  • 1. Introduction to FOXO Biology

    Wolfgang Link

     

    Part I: Biochemical and Molecular Methods

    2. Characterization of microRNAs Regulating FOXO Expression

    Doug Hanniford and Eva Hernando

     

    3. Identification of Transcription Factor Binding Sites in the Mouse FoxO1 Promoter 

    Christopher P. Cardozo

     

    4. Investigating Mechanisms that control Ubiquitin-mediated DAF-16/FOXO Protein Turnover

    Thomas Heimbucher and Coleen T. Murphy

     

    5. Phosphorylation of FOXO Proteins as a Key Mechanism to Regulate their Activity

    Alba Orea-Soufi, David Dávila, María Salazar-Roa, María de Mar Lorente, and Guillermo Velasco

     

    6. Methodological Approach for the Evaluation of FOXO as a Positive Regulator of Antioxidant Genes

    María Monsalve, Ignacio Prieto, Andreza Fabro de Bem, and Yolanda Olmos

     

    7. Characterization of FOXO Acetylation

    Shang Yao, Zimam Mahmud, Nikoleta Sachini, Sathid Aimjongjun, Paula Saavedra-García, and Eric W-F Lam

     

    8. Transcriptional Activity of FoxO Transcription Factors Measured by Luciferase Assays

    Antonia Ávila-Flores, Javier Arranz-Nicolás, and Isabel Mérida

     

    9. Monitoring the Transcriptional Activity of FOXO Transcription Factors by Analyzing their Target Genes

    Vanessa Henriques, Susana Machado, Wolfgang Link, and Bibiana I. Ferreira

     

    10. Using ChIP-based Approaches to Characterize FOXO Recruitment to its Target Promoters

    Neeraj Kumar and Arnab Mukhopadhyay

     

    11. RNAi Mediated Silencing of FoxO Factors

    Priyankar Sanphui, Akash Saha, and Subhas Chandra Biswas

     

    Part II: Imaging Approaches

    12. Immunofluorescence Analysis by Confocal Microscopy for Detecting Endogenous FOXO

    Francisco Castillo, Thomas A. Mackenzie,  and Bastien Cautain

     

    13. High Throughput Image-based Screening to Identify Chemical Compounds Capable of Activating FOXO

    Marta Barradas, Wolfgang Link, Diego Megías, and Pablo J. Fernández-Marcos

     

    14. Image-based Identification of Chemical Compounds Capable of   Trapping FOXO in the Cell Nucleus

    Susana Machado, Catarina Raposo, Bibiana I. Ferreira, and Wolfgang Link

     

    15. Quantifying Tissue-specific Overexpression of FOXO in Drosophila via mRNA Fluorescence in situ Hybridization using Branched DNA Probe Technology

    Anna C Blice-Baum, Georg Vogler, Meera C Viswanathan, Bosco Trinh, Worawan B Limpitikul, and Anthony Cammarato

     

    Part III: Omics and Bioinformatic Approaches

    16. Genome-wide Analysis for Identifying FOXO Protein Binding Sites

    Dong-Ju Shin, Pujan Joshi, Dong-Guk Shin, and Li Wang

     

    17. Mathematical Modeling of Nuclear Trafficking of  FOXO Transcription Factors

    Bradford E. Peercy and Martin F. Schneider

     

    18. Following Transcriptome to Uncover FOXO Biological Functions

    Raymond Liang, Vijay Menon, and Saghi Ghaffari

     

    Part IV: Animal Models

    19. Hydra as Model to Determine the Role of FoxO in Longevity

    Thomas C. G. Bosch

     

    20. Genetic Ablation of FOXO in Mice to Investigate its Physiological Role

    Hwanhee Oh and Jihye Paik

     

    Part V: Human Gentic and Clinical Studies

    21. Analysis of FOXO3 Gene Polymorphisms Associated with Human Longevity

    Timothy A Donlon, Philip MC Davy, and Bradley J Willcox

     

    22. Analysis of FOXO3a Gene Polymorphism Associated with Asthma

    Tejas Shah and Kalpana Joshi