Basic Cell Culture Protocols

1. Detection of Mycoplasma Contaminations.- 2. Eradication of Mycoplasma Contaminations.- 3. STR DNA Typing of Human Cell Lines: Detection of Intra- and Interspecies Cross-Contamination.- 4. Classical and Molecular Cytogenetic Analysis.- 5. Fluorescent In Situ Hybridization (FISH) of DNA Probes in the Interphase and Metaphase Stages of the Cell Cycle.- 6. The Development of T Lymphocytes in Fetal Thymus Organ Culture.- 7. Generation, Isolation, and Engraftment of In Vitro-Derived Human T Cell Progenitors.- 8. In Vitro Generation of Human T Regulatory Cells: Generation, Culture, and Analysis of FOXP3-Transduced T Cells.- 9. Simultaneous Cloning and Selection of Hybridomas and Transfected Cell Lines in Semisolid Media.- 10. Isolation and Characterization of Mouse Side Population Cells.- 11. Stem Cell Identification by DyeCycle Violet Side Population Analysis.- 12. Isolation and Characterization of Cancer Stem Cells In Vitro.- 13. Ex Vivo Differentiation of Cord Blood Stem Cells into Megakaryocytes and Platelets.- 14. Generation and Characterization of Murine Alternatively Activated Macrophages.- 15. Human Long Term Culture Initiating Cell (LTC-IC) Assay.- 16. Long Term Culture Initiating Cell (LTC-IC) Assay for Mouse Cells.- 17. Colony Forming Cell Assays for Human Hematopoietic Progenitor Cells.- 18. Studying Leukocyte Recruitment Under Flow Conditions.- 19. Generation and Establishment of Murine Adherent Cell Lines.- 20. Isolation, Enumeration, and Expansion of Human Mesenchymal Stem Cells in Culture.- 21. Isolation and Culture of Mesenchymal Stem Cells from Mouse Compact Bone .- 22. Generation of a Pool of Human Platelet Lysate (pHPL) and Efficient Use in Cell Culture.- 23. In Vitro Methods to Culture Primary Human Breast Epithelial Cells.- 24. Human Prostate Epithelial Cell Cultures.- 25. Enzymatic Dissociation, Flow Cytometric Analysis, and Culture of Normal Mouse Mammary Tissue.- 26. Isolation and Characterization of Human Hair Follicle Epithelial Cells.- 27. Co-Cultivation of Human Oral Keratinocytes and Human Osteoblast-Like Cells.- 28. Isolation and Culture of Skeletal Muscle Myofibers as a Means to Analyze Satellite Cells.- 29. Hepatic Differentiation of Embryonic Stem Cells by Murine Fetal Liver Mesenchymal Cells.- 30. Methods to Culture, Differentiate, and Characterize Neural Stem Cells from the Adult and Embryonic Mouse Central Nervous System.- 31. Feeder-Independent Culture Systems for Human Pluripotent Stem Cells.- 32. Formation of Embryoid Bodies from Human Pluripotent Stem Cells Using AggreWell™ Plates.- 33. Techniques in Embryoid Body Formation from Human Pluripotent Stem Cells.