Salamanders in Regeneration Research Methods and Protocols

Variation in Salamanders: An Essay on Genomes, Development, and Evolution.- Maintaining Eastern Newts (Notophthalmus viridescens) for Regeneration Research.- Housing and Maintenance of Ambystoma mexicanum, the Mexican Axolotl.- Husbandry of Spanish Ribbed Newts (Pleurodeles waltl.- Maintaining Plethodontid Salamanders in the Laboratory for Regeneration Studies.- Newt Lens Transdifferentiation: From Lentectomy to Immuno-FISH.- Studying Newt Brain Regeneration Following Subtype Specific Neuronal Ablation.- The Accessory Limb Model: An Alternative Experimental System of Limb Regeneration.- High-efficiency Electroporation of the Spinal Cord in Larval Axolotl.- Pseudotyped Retroviruses for Infecting Axolotl.- Thyroxine Induced Metamorphosis in the Axolotl (Ambystoma mexicanum).- Generation of Aneurogenic Larvae by Parabiosis of Salamander Embryos.- In vivo Modulation and Quantification of microRNAs During Axolotl Tail Regeneration.- Derivation and Long-Term Culture of Cells from Newt Adult Limbs and Limb Blastemas.- Culture and Transfection of Axolotl Cells.- Isolation and Culture of Neurospheres from the Adult Newt Brain.- Methods for Axolotl Blood Collection, Intravenous Injection and Efficient Leukocyte Isolation from Peripheral Blood and the Regenerating Limb.- Assessing Cardiomyocyte Proliferative Capacity in the Newt Heart and Primary Culture.-  Long Term Organ Cultures of Newt Hearts.- In vitro Preparation of Newt Inner Ear Sensory Epithelia as a Model for Repair and Regeneration .- Transgenesis in Axolotl (Ambystoma mexicanum).- Generating and Identifying Axolotls with Targeted Mutations using Cas9 RNA‐guided Nuclease.- Gene Manipulation for Regenerative Studies using the Iberian Ribbed Newt, Pleurodeles waltl.- Transcriptomics Using Axolotls.- Sal-Site: Research Resources for The Mexican Axolotl.- Data Mining in Newt-omics, the Repository for Omics Data from the Newt.