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Produktbild: Embryonic Stem Cell Protocols
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Embryonic Stem Cell Protocols

Aus der Reihe Methods in Molecular Biology
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Beschreibung

Produktdetails

Einband

Gebundene Ausgabe

Erscheinungsdatum

02.12.2015

Abbildungen

XV, 84 illus., 49 illus. in color. With online files/update., farbige Illustrationen, schwarz-weiss Illustrationen

Herausgeber

Kursad Turksen

Verlag

Springer Us

Seitenzahl

449

Maße (L/B/H)

26/18,3/2,9 cm

Gewicht

1105 g

Auflage

3rd ed. 2016

Sprache

Englisch

ISBN

978-1-4939-2953-5

Beschreibung

Produktdetails

Einband

Gebundene Ausgabe

Erscheinungsdatum

02.12.2015

Abbildungen

XV, 84 illus., 49 illus. in color. With online files/update., farbige Illustrationen, schwarz-weiss Illustrationen

Herausgeber

Kursad Turksen

Verlag

Springer Us

Seitenzahl

449

Maße (L/B/H)

26/18,3/2,9 cm

Gewicht

1105 g

Auflage

3rd ed. 2016

Sprache

Englisch

ISBN

978-1-4939-2953-5

Herstelleradresse

Springer-Verlag GmbH
Tiergartenstr. 17
69121 Heidelberg
DE

Email: GPSR Kontakt

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  • Produktbild: Embryonic Stem Cell Protocols

  • 1. Maximizing Clonal Embryonic Stem Cell Derivation by ERK Pathway Inhibition

                Jennifer Nichols and Thorsten Boroviak

     

    2. A Simple and Efficient Method of Slow Freezing for Human Embryonic Stem Cells and Induced Pluripotent Stem Cells

                Keitaro Imaizumi, Momoe Iha, Naoki Nishishita, Shin Kawamata, Shinichi Nishikawa, and Teruo Akuta

     

    3. Resolving Heterogeneity: Fluorescence Activated Cell Sorting of Dynamic Cell Populations from Feeder-Free Mouse Embryonic Stem Cell Culture

                Jurriaan J. Hölzenspies, Gelo V. Dela Cruz, and Joshua M. Brickman

     

    4. Acquiring Ground State Pluripotency: Switching Mouse Embryonic Stem Cells from Serum/LIF Medium to 2i/LIF Medium

                Matteo Tosolini and Alice Jouneau

     

    5. Generation of Embryonic Stem Cells in Rabbits

                Marielle Afanassieff, Pierre Osteil, and Pierre Savatier

     

    6. Gene Transfer into Pluripotent Stem Cells Via Lentiviral Transduction

                Ortwin Naujok, Ulf Diekmann, and Matthias Elsner

     

    7. Imaging Pluripotency: Time-Lapse Analysis of Mouse Embryonic Stem Cells

                Anna Pezzarossa, Ana M.V. Guedes, Domingos Henrique, and Elsa Abranches

     

    8. Expanding the Utility of FUCCI Reporters Using FACS-Based ‘Omics Analysis

                James Chappell, Ben Boward, and Stephen Dalton

     

    9. Selection of Antibodies Interfering with Cell-Surface Receptor Signalling Using Embryonic Stem Cell Differentiation

                Anna N. Melidoni, Michael R. Dyson, and John McCafferty

     

    10. Delivering Antisense Morpholino Oligonucleotides to Target Telomerase Splice Variants in Human Embryonic Stem Cells

                Lida Radan, Chris S. Hughes, Jonathan H. Teichroeb, Lynne-Marie Postovit, and Dean H. Betts

     

    11. Analysis of mRNA Translation Rate in Mouse Embryonic Stem Cells

                Anisa B. Rahim and Leah A. Vardy

     

    12. Generation and Purification of Definitive Endoderm Cells Generated from Pluripotent Stem Cells

                Ulf Diekmann and Ortwin Naujok

     

    13. Definitive Endoderm Differentiation of Human Embryonic Stem Cells Combined with Selective Elimination of Undifferentiated Cells by Methionine Deprivation

                Tomonori Tsuyama, Nobuaki Shiraki, and Shoen Kume

     

    14. Selective Differentiation into Hematopoietic and Cardiac Cells from Pluripotent Stem Cells Based on the Expression of Cell Surface Markers

                Atsumasa Okada, Katsuhisa Tashiro, Tomoko Yamaguchi, and Kenji Kawabata

     

    15. Methods for Derivation of Multipotent Neural Crest Cells Derived from Human Pluripotent Stem Cells

                John Avery and Stephen Dalton

     

    16. From Naive to Primed Pluripotency: In Vitro Conversion of Mouse Embryonic Stem Cells in Epiblast Stem Cells

                Matteo Tosolini and Alice Jouneau

     

    17. In Vitro Differentiation of Embryonic Stem Cells into Hematopoietic Lineage: Towards Erythroid Progenitor’s Production

                Iliana Fauzi, Nicki Panoskaltsis, and Athanasios Mantalaris

     

    18. Dopaminergic Differentiation of Human Embryonic Stem Cells on PA6-Derived Adipocytes

                M. Oktar Guloglu and Anna Larsen

     

    19. Intracellular Calcium Measurements for Functional Characterization of Neuronal Phenotypes

                Talita Glaser, Ana Regina G. Castillo, Ágatha Oliveira, and Henning Ulrich

     

    20. In Vitro Differentiation of Pluripotent Stem Cells into Functional β Islets Under 2D and 3D Culture Conditions and In Vivo Preclinical Validation of 3D Islets

                Bipasha Bose and Sudheer Shenoy P

     

    21. Generation of Corneal Keratocytes from Human Embryonic Stem Cells

                Andrew J. Hertsenberg and James L. Funderburgh

     

    22. Maintenance, Transgene Delivery, and Pluripotency Measurement of Mouse Embryonic Stem Cells

                Tetsuya S. Tanaka

     

    23. Generation of a Knockout Mouse Embryonic Stem Cell Line Using a Paired CRISPR/Cas9 Genome Engineering Tool

                Rahel Wettstein, Maxime Bodak, and Constance Ciaudo

     

    24. Derivation of Neural Precursor Cells from Human Embryonic Stem Cells for DNA Methylomic Analysis

                Ivan Roubal, Sun Joo Park, and Yong Kim

     

    25. 26S and PA28-20S Proteasome Activity in Cytosolic Extracts from Embryonic Stem Cells

                Malin Hernebring

     

    26. A Concise Protocol for siRNA-Mediated Gene Suppression in Human Embryonic Stem Cells

                Peter F. Renz and Tobias A. Beyer

     

    27. Applying Shear Stress to Pluripotent Stem Cells

                Russell P. Wolfe, Julia B. Guidry, Stephanie L. Messina, and Tabassum Ahsan

     

    28. Generating Inner Ear Organoids from Mouse Embryonic Stem Cells

                Emma Longworth-Mills, Karl R. Koehler, and Eri Hashino

     

    29. Differentiation of Adipocytes in Monolayer from Mouse Embryonic Stem Cells

                Ixchelt Cuaranta-Monroy, Zoltan Simandi, and Laszlo Nagy

     

    30. Pancreatic Differentiation from Murine Embryonic Stem Cells

                Daisuke Sakano, Nobuaki Shiraki, and Shoen Kume

     

    31. Osteogenic Differentiation from Embryonic Stem Cells

                Yanhong Yu, Carlos Pilquil, and Michal Opas

     

    32. Limbal Stromal Tissue Specific Stem Cells and Their Differentiation Potential to Corneal Epithelial Cells

                Kishore Reddy Katikireddy and Ula V. Jurkunas