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Produktbild: Transgenesis and the Management of Vector-Borne Disease
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Transgenesis and the Management of Vector-Borne Disease

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Produktdetails

Format

PDF

Kopierschutz

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Erscheinungsdatum

21.08.2008

Herausgeber

Serap Aksoy

Verlag

Springer New York

Seitenzahl

171 (Printausgabe)

Dateigröße

16386 KB

Sprache

Englisch

EAN

9780387782256

Beschreibung

Produktdetails

Format

PDF

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Erscheinungsdatum

21.08.2008

Herausgeber

Serap Aksoy

Verlag

Springer New York

Seitenzahl

171 (Printausgabe)

Dateigröße

16386 KB

Sprache

Englisch

EAN

9780387782256

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  • Produktbild: Transgenesis and the Management of Vector-Borne Disease
  • 1. PERSPECTIVES ON THE STATE OF INSECT TRANSGENICS ............ 1 David A. O'Brochta and Alfred M. Handler Introduction ......................................................................................................................... 1 Transposon Vectors and Insect Transformation ............................................................... 2 Transformation Markers .................................................................................................... 4 Targeting and Stabilization ................................................................................................. 5 Random Transgene Insertion ............................................................................................. 5 Transgenic Strains for Biocontrol ...................................................................................... 8 Fluorescent Protein Genetic Markers ................................................................................ 8 Conditional Regulation for Sterility and Lethality .......................................................... 9 Paratransgenesis ................................................................................................................ 10 Viruses ............................................................................................................................... 10 Perspectives on TE Spread and Genetic Manipulation for Disease Vector Control ... 11 Broadening Our Perspective ............................................................................................. 12 2. ALPHAVIRUS TRANSDUCING SYSTEMS .............................................. 19 Brian D. Foy and Ken E. Olson Introduction ....................................................................................................................... 19 Alphaviruses ....................................................................................................................... 20 ATS Construction .............................................................................................................. 22 Currently Used ATSs ......................................................................................................... 22 Alphavirus/Mosquito Interactions ................................................................................... 24 Gene Expression with ATSs .............................................................................................. 27 ATS s for Induction of RNA Interference ....................................................................... 27 Biosafety Considerations for Using ATS's ....................................................................... 29 Conclusion .......................................................................................................................... 30 3. PARATRANSGENESIS APPLIED FOR CONTROL OF TSETSE TRANSMITTED SLEEPING SICKNESS.................. 35 Serap Aksoy, Brian Weiss and Geoffrey Attardo Introduction ....................................................................................................................... 35 Symbiosis in Tsetse ............................................................................................................ 36 Symbiont-Host Interactions During Development ......................................................... 37 Tsetse's Reproductive Biology and Symbiont Transmission .......................................... 38 Biology of African Trypanosome Transmission in Tsetse .............................................. 38 Paratransgenic Gene Expression in Tsetse ...................................................................... 39 Reconstitution of Tsetse with Modified Symbiont Flora ................................................ 41 Effector Genes with Trypanocidal Activity ..................................................................... 42 Gene Driver System ........................................................................................................... 43 Applications with Parasite Resistant Tsetse .................................................................... 43 Application of Paratransgenesis for Control of Other Insect Transmitted Diseases ...................................................................................... 44 Future Directions ............................................................................................................... 45 4. BACTERIA OF THE GENUS ASAIA: A POTENTIAL PARATRANSGENIC WEAPON AGAINST MALARIA ................ 49 Guido Favia, Irene Ricci, Massimo Marzorati, Ilaria Negri, Alberto Alma, Luciano Sacchi, Claudio Bandi, and Daniele Daffonchio Introduction ....................................................................................................................... 49 Malaria and Symbiotic Control Strategies ...................................................................... 50 a-Proteobacteria of the Genus Asaia Dominate the Microflora of An. stephensi ........................................................................................................... 51 Asaia is Localized in Different Organs of An. stephensi ................................................ 53 Asaia: A Self-Spreading Carrier of Potential Antagonistic Factors in Mosquitoes ..... 55 Conclusions and Perspectives ........................................................................................... 56 5. PROPOSED USES OF TRANSPOSONS IN INSECT AND MEDICAL BIOTECHNOLOGY ........................ 60 Peter W. Atkinson Introduction ....................................................................................................................... 60 Genetic Control Strategies and Transposon Immobility ............................................... 63 Transformation Efficiency ................................................................................................ 63 Target Site Preference ....................................................................................................... 65 Post Integration Stability .................................................................................................. 65 Regulation of Transposition by piRNA ........................................................................... 66 Genetic Control Strategies and Transposon Drive ......................................................... 67 Conclusions ........................................................................................................................ 68 6. THE YIN AND YANG OF LINKAGE DISEQUILIBRIUM: MAPPING OF GENES AND NUCLEOTIDES CONFERRING INSECTICIDE RESISTANCE IN INSECT DISEASE VECTORS ..................................................... 71 William C. Black IV, Norma Gorrochetegui-Escalante, Nadine P. Randle and Martin J. Donnelly Abstract .............................................................................................................................. 71 Mapping of Genome Regions and SNPs Conferring Insecticide Resistance ............... 72 Terminology in Linkage Disequilibrium and QTN Mapping ........................................ 73 Measuring Linkage Disequilibrium ................................................................................. 73 Contents xvii Patterns of Linkage Disequilibrium in Vectors .............................................................. 74 The Yin and Yang of Linkage Disequilibrium ................................................................ 77 QTL Mapping .................................................................................................................... 78 Microarray Technology ..................................................................................................... 79 7. IMPACT OF TECHNOLOGICAL IMPROVEMENTS ON TRADITIONAL CONTROL STRATEGIES ............................. 84 Mark Q. Benedict and Alan S. Robinson Introduction ....................................................................................................................... 84 Applications ....................................................................................................................... 85 Are Transgenic Insects Compatible Partners in the IPM Mix? .................................... 90 Conclusions ........................................................................................................................ 90 8. INSECT POPULATION SUPPRESSION USING ENGINEERED INSECTS ..................................................... 93 Luke Alphey, Derric Nimmo, Sinead O'Connell and Nina Alphey Introduction ....................................................................................................................... 93 Genetic Sexing and Genetic Sterilization ........................................................................ 95 Molecular Biology of Repressible Lethal Systems ........................................................ 100 Regulatory Issues and Concluding Remarks ................................................................ 100 9. WOLBACHIA-BASED TECHNOLOGIES FOR INSECT PEST POPULATION CONTROL ................................................... 104 Kostas Bourtzis Introduction ..................................................................................................................... 104 Wolbachia Induced Cytoplasmic Incompatibility ......................................................... 105 Wolbachia-Based Applications ........................................................................................ 106 Conclusions and Future Challenges ............................................................................... 109 10. USING PREDICTIVE MODELS TO OPTIMIZE WOLBACHIA-BASED STRATEGIES FOR VECTOR-BORNE DISEASE CONTROL ............................ 114 Jason L. Rasgon Introduction ..................................................................................................................... 114 Wolbachia Endosymbionts ............................................................................................. 115 More Realistic Population Dynamics of Wolbachia Infections .................................... 118 Can Modeling Highlight a Better Way to Control Disease Using Wolbachia Infections? ................................................................................................................ 119 Using Wolbachia to Drive Nuclear Traits? .................................................................... 122 Conclusions ...................................................................................................................... 124