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Produktbild: Genetic Theory and Analysis
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Genetic Theory and Analysis Finding Meaning in a Genome

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69,99 € UVP 95,90 €

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Beschreibung

Produktdetails

Einband

Taschenbuch

Erscheinungsdatum

29.08.2023

Verlag

John Wiley & Sons Inc

Seitenzahl

304

Maße (L/B/H)

25,3/17,6/1,7 cm

Gewicht

644 g

Auflage

2nd edition

Sprache

Englisch

ISBN

978-1-118-08692-6

Beschreibung

Produktdetails

Einband

Taschenbuch

Erscheinungsdatum

29.08.2023

Verlag

John Wiley & Sons Inc

Seitenzahl

304

Maße (L/B/H)

25,3/17,6/1,7 cm

Gewicht

644 g

Auflage

2nd edition

Sprache

Englisch

ISBN

978-1-118-08692-6

Herstelleradresse

Libri GmbH
Europaallee 1
36244 Bad Hersfeld
DE

Email: GPSR Kontakt

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  • Produktbild: Genetic Theory and Analysis
  • Preface xi
     
    Introduction xiii
     
    1 Mutation 1
     
    1.1 Types of Mutations 1
     
    Muller's Classification of Mutants 2
     
    Nullomorphs 2
     
    Hypomorphs 4
     
    Hypermorphs 5
     
    Antimorphs 6
     
    Neomorphs 8
     
    Modern Mutant Terminology 10
     
    Loss-of-Function Mutants 10
     
    Dominant Mutants 10
     
    Gain-of-Function Mutants 11
     
    Separation-of-Function Mutants 11
     
    DNA-Level Terminology 11
     
    Base-Pair-Substitution Mutants 11
     
    Base-Pair Insertions or Deletions 12
     
    Chromosomal Aberrations 12
     
    1.2 Dominance and Recessivity 13
     
    The Cellular Meaning of Dominance 13
     
    The Cellular Meaning of Recessivity 15
     
    Difficulties in Applying the Terms Dominant and Recessive to Sex-Linked Mutants 16
     
    The Genetic Utility of Dominant and Recessive Mutants 17
     
    1.3 Summary 17
     
    References 17
     
    2 Mutant Hunts 20
     
    2.1 Why Look for New Mutants? 20
     
    Reason 1: To Identify Genes Required for a Specific Biological Process 21
     
    Reason 2: To Isolate more Mutations in a Specific Gene of Interest 31
     
    Reason 3: To Obtain Mutants for a Structure-Function Analysis 32
     
    Reason 4: To Isolate Mutations in a Gene So Far Identified only by Computational Approaches 32
     
    2.2 Mutagenesis and Mutational Mechanisms 32
     
    Method 1: Ionizing Radiation 33
     
    Method 2: Chemical Mutagens 33
     
    Alkylating Agents 34
     
    Crosslinking Agents 35
     
    Method 3: Transposons 35
     
    Identifying Where Your Transposon Landed 37
     
    Why not Always Screen With TEs? 40
     
    Method 4: Targeted Gene Disruption 40
     
    RNA Interference 40
     
    CRISPR/Cas9 41
     
    TALENs 42
     
    So Which Mutagen Should You Use? 43
     
    2.3 What Phenotype Should You Screen (or Select) for? 44
     
    2.4 Actually Getting Started 45
     
    Your Starting Material 45
     
    Pilot Screen 45
     
    What to Keep? 45
     
    How many Mutants is Enough? 46
     
    Estimating the Number of Genes not Represented by Mutants in Your New Collection 46
     
    2.5 Summary 48
     
    References 48
     
    3 Complementation 51
     
    3.1 The Essence of the Complementation Test 51
     
    3.2 Rules for Using the Complementation Test 55
     
    The Complementation Test Can be Done Only When Both Mutants are Fully Recessive 55
     
    The Complementation Test Does Not Require that the Two Mutants Have Exactly the Same Phenotype 56
     
    The Phenotype of a Compound Heterozygote Can be More Extreme than that of Either Homozygote 56
     
    3.3 How the Complementation Test Might Lie to You 57
     
    Two Mutations in the Same Gene Complement Each Other 57
     
    A Mutation in One Gene Silences Expression of a Nearby Gene 57
     
    Mutations in Regulatory Elements 59
     
    3.4 Second-Site Noncomplementation (Nonallelic Noncomplementation) 59
     
    Type 1 SSNC (PoisonousInteractions): The Interaction is Allele Specific at Both Loci 60
     
    An Example of Type 1 SSNC Involving the Alpha- and Beta-Tubulin Genes in Yeast 60
     
    An Example of Type 1 SSNC Involving the Actin Genes in Yeast 62
     
    Type 2 SSNC (Sequestration): The Interaction is Allele Specific at One Locus 66
     
    An Example of Type 2 SSNC Involving the Tubulin Genes in Drosophila 66
     
    An Example of Type 2 SSNC in Drosophila that Does Not Involve the Tubulin Genes 69
     
    An Example of Type 2 SSNC in the Nematode Caenorhabditis elegans 71
     
    Type 3 SSNC (Combined Haploinsufficiency): The Interaction is Allele-Independent at Both Loci 72
     
    An Example of Ty