Produktbild: Molecular Methods for Evolutionary Genetics
Band 772

Molecular Methods for Evolutionary Genetics

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Beschreibung

Produktdetails

Einband

Gebundene Ausgabe

Erscheinungsdatum

29.09.2011

Abbildungen

XVI, 503 p. 81 illus., 4 illus. in color.

Herausgeber

Virginie Orgogozo + weitere

Verlag

Humana Press

Seitenzahl

503

Maße (L/B/H)

26/18,3/3,3 cm

Gewicht

1165 g

Auflage

2011

Sprache

Englisch

ISBN

978-1-61779-227-4

Beschreibung

Produktdetails

Einband

Gebundene Ausgabe

Erscheinungsdatum

29.09.2011

Abbildungen

XVI, 503 p. 81 illus., 4 illus. in color.

Herausgeber

Verlag

Humana Press

Seitenzahl

503

Maße (L/B/H)

26/18,3/3,3 cm

Gewicht

1165 g

Auflage

2011

Sprache

Englisch

ISBN

978-1-61779-227-4

Herstelleradresse

Springer-Verlag GmbH
Tiergartenstr. 17
69121 Heidelberg
DE

Email: ProductSafety@springernature.com

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  • Produktbild: Molecular Methods for Evolutionary Genetics

  • Genome Size Determination using Flow Cytometry of Propidium Iodide-stained Nuclei.- Chromosome Analysis in Invertebrates and Vertebrates.- Genomic Libraries. I. Construction and Screening of Fosmid Genomic Libraries.- Genomic Libraries. II. Subcloning, Sequencing, and Assembling Large-insert Genomic DNA Clones.- Reduced Representation Methods for Sub-genomic Enrichment and Next-generation Sequencing.- Accessing the Transcriptome: How to Normalize mRNA Pools.- Transcriptome Sequencing Goals, Assembly, and Assessment.- Rapid Retrieval of DNA Target Sequences by Primer Extension Capture.- SNP Discovery and Genotyping for Evolutionary Genetics using RAD Sequencing.- DNA Microarray-based Mutation Discovery and Genotyping.- Genotyping with Sequenom.- Isolating Microsatellite Loci: Looking Back, Looking Ahead.- Design of Custom Oligonucleotide Microarrays for Single Species or Interspecies Hybrids using Array Oligo Selector.- Identification of Homologous Gene Sequences by PCR with Degenerate Primers.- Characterizing cDNA Ends by Circular RACE.- Identification of DNA Sequences that Flank a Known Region by Inverse PCR.- Quantification of Transcript Levels with Quantitative RT-PCR.- Using Pyrosequencing to Measure Allele-specific mRNA Abundance and Infer the Effects of Cis- and Trans-regulatory Differences.- Whole Mount In situ Hybridization of Sectioned Tissues of Species Hybrids to Detect Cis-Regulatory Changes in Gene Expression Pattern.- Identifying Fluorescently Labeled Single Molecules in Image Stacks using Machine Learning.- Experimental Approaches to Evaluate the Contributions of Candidate Cis-regulatory Mutations to Phenotypic Evolution.- Experimental Approaches to Evaluate the Contributions of Candidate Protein-coding Mutations to Phenotypic Evolution.- Making Reporter Gene Constructs to Analyze Cis-regulatory Elements.- PCR-directed In vivo Plasmid Construction using Homologous Recombination in Baker’s Yeast.- Production of Fosmid Genomic Libraries Optimized for Liquid Culture Recombineering and Cross-species Transgensis.- Recombination-mediated Genetic Engineering of Large Genomic DNA Transgenes.- Overlap Extension PCR: An Efficient Method for Transgene Construction.- Gene Knockdown Analysis by Double-stranded RNA Injection