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Produktbild: CANCER CELL CULTURE
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CANCER CELL CULTURE

Aus der Reihe Methods in Molecular Medicine
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Beschreibung

Produktdetails

Einband

Taschenbuch

Abbildungen

98 SW-Abb.

Herausgeber

Simon P. Langdon

Verlag

Springer

Seitenzahl

368

Maße (L/B/H)

2,1/15,2/22,9 cm

Sprache

Englisch

ISBN

978-1-61737-343-5

Beschreibung

Produktdetails

Einband

Taschenbuch

Abbildungen

98 SW-Abb.

Herausgeber

Simon P. Langdon

Verlag

Springer

Seitenzahl

368

Maße (L/B/H)

2,1/15,2/22,9 cm

Sprache

Englisch

ISBN

978-1-61737-343-5

Herstelleradresse

Springer Heidelberg
Tiergartenstr. 17
69121 Heidelberg
DE
buchhandel-buch@springer.com

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  • Produktbild: CANCER CELL CULTURE
  • Part I. Introduction to Cancer Cell Culture
    Basic Principles of Cancer Cell Culture
    Simon P. Langdon
    Essential Techniques of Cancer Cell Culture
    Kenneth G. Macleod and Simon P. Langdon
    Part II. Characterization and Authentication
    Characterization and Authentication of Cancer Cell Lines: An Overview
    Simon P. Langdon
    Authentication of Cancer Cell Lines by DNA Fingerprinting
    Wilhelm G. Dirks and Hans G. Drexler
    Cytogenetic Characterization of Tumor Cell Lines
    Roderick A. F. MacLeod and Hans G. Drexler
    Part III. Isolation and Culture of Specialized Cell Types
    Isolation and Culture of Colon Cancer Cell Lines
    Jae-Gahb Park, Ja-Lok Ku, and So-Yeon Park
    Isolation and Culture of Melanoma Cell Lines
    Adam I. Riker
    Isolation and Culture of Human Brain Tumor Cells
    German G. Gomez and Carol A. Kruse
    Isolation and Culture of Renal Cancer Cell Lines
    Jae-Gahb Park, Ja-Lok Ku, and So-Yeon Park
    Isolation and Culture of Prostate Cancer Cell Lines
    Nora M. Navone, Matilde Olive, and Patricia Troncoso
    Isolation and Culture of Ovarian Cancer Cell Lines
    Simon P. Langdon
    Isolation and Culture of Leukemia Cell Lines
    Hans G. Drexler
    Part IV. Functional Assays in Cell Culture
    Cell Sensitivity Assays: Clonogenic Assay
    Jane A. Plumb
    Cell Sensitivity Assays: The MTT Assay
    Jane A. Plumb
    PARP Cleavage as a Means of Assessing Apoptosis
    Peter Mullen
    Detection of Apoptosis by the TUNEL Assay
    John Lawry
    Apoptosis Measurement by Annexin V Staining
    Eric Miller
    Cell Adhesion Assays
    William N. Scott
    Radial Monolayer Cell Migration Assay
    Michael E. Berens and Christian Beaudry
    Invasion and Motility Assays
    William N. Scott
    Identification of Senescence in Cancer Cells
    Lesley J. Jardine
    Angiogenesis Assays
    William N. Scott
    Flow Cytometric DNA Analysis of Human Cancer Cell Lines
    Peter Mullen
    Part V. Modification of Cancer Cells
    DNA-Mediated Gene Transfer
    Amanda J. M. O'Donnell
    Development of Drug-Resistant Models
    Helen M. Coley
    Immortalization of Human Prostate Cells With the Human Papillomavirus Type 16 E6 Gene
    Norman J. Maitland, Catherine A. Macintosh, Christian Schmitz, and Shona H. Lang
    The Use of Matrigel to Facilitate the Establishment of Human Cancer Cell Lines as Xenografts
    Peter Mullen
    Part VI. Coculture Systems
    Coculture of Prostate Cancer Cells
    Shona H. Lang and Norman J. Maitland
    Coculture of Ovarian Cells Using Porous Tissue Culture Inserts
    Katherine E. Snow and Simon P. Langdon
    Part VII. Cell Line Contamination
    Cell Culture Contamination: An Overview
    Simon P. Langdon
    Detecting Mycoplasma Contamination in Cell Cultures by Polymerase Chain Reaction
    Cord C. Uphoff and Hans G. Drexler
    Elimination of Mycoplasma from Infected Cell Lines Using Antibiotics
    Cord C. Uphoff and Hans G. Drexler
    Appendix 1: Formulations of Commonly Used Cell Culture Media
    Appendix 2: Human Cancer Cell Lines Available from the ATCC and DSMZ Cell Banks (Combined List)
    Index