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Produktbild: Plant Gene Transfer and Expression Protocols
Band 49

Plant Gene Transfer and Expression Protocols

Aus der Reihe Methods in Molecular Biology

138,99 €

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Beschreibung

Produktdetails

Einband

Spiralbindung

Erscheinungsdatum

27.09.1995

Verlag

Humana Press

Seitenzahl

466

Maße (L/B/H)

22,6/17/2,8 cm

Gewicht

658 g

Auflage

1996 edition

Sprache

Englisch

ISBN

978-0-89603-321-4

Beschreibung

Rezension

The chapters were written by outstanding scientists and [the book] can be recommended to any laboratory working with plants, providing tips and alternatives in case of problems. -Journal of Plant Physiology

Produktdetails

Einband

Spiralbindung

Erscheinungsdatum

27.09.1995

Verlag

Humana Press

Seitenzahl

466

Maße (L/B/H)

22,6/17/2,8 cm

Gewicht

658 g

Auflage

1996 edition

Sprache

Englisch

ISBN

978-0-89603-321-4

Herstelleradresse

Libri GmbH
Europaallee 1
36244 Bad Hersfeld
DE

Email: GPSR Kontakt

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  • Produktbild: Plant Gene Transfer and Expression Protocols
  • Part I: Agrobacterium-Mediated Transformation. Tools for Expressing Foreign Genes in Plants. Introduction of Cloning Plasmids into Agrobacterium tumefaciens. Leaf Disk Transformation Using Agrobacterium tumefaciens-Expression of Heterologous Genes in Tobacco. Agrobacterium rhizogenes as a Vector for Transforming Higher Plants: Application in Lotus corniculatus Transformation. Agrobacterium-Mediated Transformation of Arabidopsis thaliana: Application in T-DNA Tagging. Agrobacterium-Mediated Transfer of Geminiviruses to Plant Tissues. Part II: Direct Gene Transfer. Stable Transformation of Barley via Direct DNA Uptake: Electroporation- and PEG-Mediated Protoplast Transformation. Gene Transfer into Plant Protoplasts by Electroporation. Transformation of Cereals by Microprojectile Bombardment of Immature Inflorescence and Scutellum Tissues. Part III: Use of Reporter Genes. The b-Glucoronidase (gus) Reporter Gene System: Gene Fusions, Spectrophotometric, Fluorometric, and Histochemical Detection. Chloramphenicol Acetyl Transferase Assay. NPTII Assays for Measuring Gene Expression and Enzyme Activity in Transgenic Plants. Part IV: Study of Gene Organization by Southern Blotting and Inverse PCR. Gene Characterization by Southern Analysis. Isolation and Characterization of Plant Genomic DNA Sequences via (Inverse) PCR Amplification. Part V: RNA Techniques for Studying Gene Expression. Isolation of Whole Cell (Total) RNA. Poly(A)+ RNA Isolation. In Vitro Translation. Northern Analysis and Nucleic Acid Probes. Nuclear 'Run-On' Transcription Assays. RNase A/T1 Protection Assay. Primer Extension Assay. Applications of RT-PCR. In Vitro Transcription of Class II Promoters in Higher Plants. Analysis of Plant Gene Expression by Reverse Transcription-PCR. In Situ Hybridization to Plant Tissue Sections. Xenopus Oocytes as a Heterologous Expression System. Heterologous Expression in Yeast. Part VI: Techniques for Studying Chloroplast Gene Expression. The Isolation of Intact Chloroplasts. In Vitro Protein Import by Isolated Chloroplasts. Targeting of Foreign Proteins to the Chloroplast. Part VII: Techniques for Studying Mitochondrial Gene Expression. Isolation of Mitochondria. Mitochondrial Nucleic Acid Purification and Analysis. In organello Protein Synthesis. Part VIII: Immunological Detection of Proteins. Separation of Plant Proteins by Electrophoresis. Western Blotting Analysis. ELISA Detection of Foreign Proteins. Immunocytochemical Localization of Proteins. Index.