Produktbild: PCR Cloning Protocols
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PCR Cloning Protocols

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Beschreibung

Produktdetails

Einband

Gebundene Ausgabe

Erscheinungsdatum

01.04.2002

Abbildungen

1, black & white illustrations

Herausgeber

Bing-Yuan Chen + weitere

Verlag

Humana Press Inc.

Seitenzahl

439

Maße (L/B/H)

23,5/15,5/2,3 cm

Gewicht

1 g

Auflage

2. Auflage

Sprache

Englisch

ISBN

978-0-89603-973-5

Beschreibung

Zitat

"This hardcover book is packed full of detailed protocols and methodological articles on various aspects of PCR. The fact that the book was taken out of my hands by a colleague, to read up on touch-down PCR, as soon as I received it for review, attest to the book's usefulness. I will have to chain this book to my bookshelf, to make sure it does not disappear quickly-or I may consider buying a couple for the laboratory." -Plant Science Review of the first edition. This volume provides protocols useful to both the inexperienced and the experienced investigator. Most importantly it highlights the emerging role of PCR as a versatile tool for the molecular biologist eliminating many laborious and expensive techniques associated with traditional gene isolation and analysis.-Biochemical Education

Produktdetails

Einband

Gebundene Ausgabe

Erscheinungsdatum

01.04.2002

Abbildungen

1, black & white illustrations

Herausgeber

Verlag

Humana Press Inc.

Seitenzahl

439

Maße (L/B/H)

23,5/15,5/2,3 cm

Gewicht

1 g

Auflage

2. Auflage

Sprache

Englisch

ISBN

978-0-89603-973-5

Herstelleradresse

Springer Heidelberg
Tiergartenstr. 17
69121 Heidelberg
DE
[email protected]

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  • Produktbild: PCR Cloning Protocols
  • Part I. Performing and Optimizing PCR

    Polymerase Chain Reaction: Basic Principles and Routine Practice

    Lori A. Kolmodin and David E. Birch

    Computer Programs for PCR Primer Design and Analysis

    Bing-Yuan Chen, Harry W. Janes, and Steve Chen

    Single-Step PCR Optimization Using Touchdown and Stepdown PCR Programming

    Kenneth H. Roux

    XL PCR Amplification of Long Targets from Genomic DNA

    Lori A. Kolmodin

    Coupled One-Step Reverse Transcription and Polymerase Chain Reaction Procedure for Cloning Large cDNA Fragments

    Jyrki T. Aatsinki

    Long Distance Reverse-Transcription PCR

    Volker Thiel, Jens Herold, and Stuart G. Siddell

    Increasing PCR Sensitivity for Amplification from Paraffin-Embedded Tissues

    Abebe Akalu and Juergen K. V. Reichardt

    GC-Rich Template Amplification by Inverse PCR: DNA Polymerase and Solvent Effects

    Alain Moreau, Da Shen Wang, Steve Forget, Colette Duez, and Jean Dusart

    PCR Procedure for the Isolation of Trinucleotide Repeats

    Teruaki Tozaki

    Methylation-Specific PCR

    Haruhiko Ohashi

    Direct Cloning of Full-Length Cell Differentially Expressed Genes by Multiple Rounds of Subtractive Hybridization Based on Long-Distance PCR and Magnetic Beads

    Xin Huang, Zhenglong Yuan, and Xuetao Cao

    Part II. Cloning PCR Products

    Cloning PCR Products: An Overview

    Baotai Guo and Yuping Bi

    Using T4 DNA Polymerase to Generate Clonable PCR Products

    Kai Wang

    Enzyme-Free Cloning of PCR Products and Fusion Protein Expression

    Brett A. Neilan and Daniel Tillett

    Directional Restriction Site-Free Insertion of PCR Products into Vectors

    Guo Jun Chen

    Autosticky PCR: Directional Cloning of PCR Products with Preformed 5' Overhangs

    József Gál and Miklós Kálmán

    A Rapid and Simple Procedure for Direct Cloning of PCR Products into Baculoviruses

    Tamara S. Gritsun, Michael V. Mikhailov, and Ernest A. Gould

    Part III. Mutagenesis and Recombination

    PCR Approaches to DNA Mutagenesis and Recombination: An Overview

    Binzhang Shen

    In-Frame Cloning of Synthetic Genes Using PCR Inserts

    James C. Pierce

    Megaprimer PCR

    Sailen Barik

    PCR-Mediated Recombination: A General Method Applied to Construct Chimeric Infectious Molecular Clones

    Guowei Fang, Barbara Weiser, Aloise Visosky, Timothy Moran, and Harold Burger

    PCR Method for Generating Multiple Mutations at Adjacent Sites

    Jiri Adamec

    A Fast Polymerase Chain Reaction-Mediated Strategy for Introducing Repeat Expansions into CAG-Repeat Containing Genes

    Franco Laccone

    PCR Screening in Signature-Tagged Mutagenesis of Essential Genes

    Dario E. Lehoux and Roger C. Levesque

    Staggered Extension Process (StEP) In Vitro Recombination

    Anna Marie Aguinaldo and Frances Arnold

    Random Mutagenesis by Whole-Plasmid PCR Amplification

    Donghak Kim and F. Peter Guengerich

    Part IV. Cloning Unknown Neighboring DNA

    PCR-Based Strategies to Clone Unknown DNA Regions from Known Foreign Integrants: An Overview

    Eric Ka-Wai Hui, Po-Ching Wang, and Szecheng J. Lo

    Long Distance Vectorette PCR (LDV PCR)

    James A. L. Fenton, Guy Pratt, and Gareth J. Morgan

    Nonspecific, Nested Suppression PCR Method for Isolation of Unknown Flanking DNA ("Cold-Start Method")

    Michael Lardelli

    Inverse PCR: cDNA Cloning

    Sheng-He Huang

    Inverse PCR: Genomic DNA Cloning

    Ambrose Y. Jong, Anna T'ang, De-Pei Liu, and Sheng-He Huang

    Gene Cloning and Expression Profiling by Rapid Amplification of Gene Inserts with Universal Vector Primers

    Sheng-He Huang, Hua-Yang Wu, and Ambrose Y. Jong

    The Isolation of DNA Sequences Flanking Tn5 Transposon Insertions by Inverse PCR

    Vincent J. J. Martin and William W. Mohn

    Rapid Amplification of Genomic DNA Sequences Tagged by Insertional Mutagenesis

    Martina Celerin and Kristin T. Chun

    Isolation of Large Terminal Sequences of BAC Inserts Based on Double-Restriction-Enzyme Digestion Followed by Anchored PCR

    Zhong-Nan Yang and T. Erik Mirkov

    A "Step Down" PCR-Based Technique for Walking Into and the Subsequent Direct Sequence Analysis of Flanking Genomic DNA

    Ziguo Zhang and Sarah Jane Gurr

    Part V. Library Construction and Screening

    Use of PCR in Library Screening: An Overview

    Jinbao Zhu

    Cloning of Homologous Genes by Gene-Capture PCR

    Renato Mastrangeli and Silvia Donini

    Rapid and Nonradioactive Screening of Recombinant Libraries by PCR

    Michael W. King

    Rapid cDNA Cloning by PCR Screening (RC-PCR)

    Toru Takumi

    Generation and PCR Screening of Bacteriophage l Sublibraries Enriched for Rare Clones (the "Sublibrary Method")

    Michael Lardelli

    PCR-Based Screening for Bacterial Artificial Chromosome Libraries

    Yuji Yasukochi

    A 384-Well Microtiter-Plate-Based Template Preparation and Sequencing Method

    Lei He and Kai Wang

    A Microtiter-Plate-Based High Throughput PCR Product Purification Method

    Ryan Smith and Kai Wang